Allele Dmel\His3^{SL.HDE.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\His3^{SL.HDE.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0221473
Feature type	allele	Associated gene	Dmel\His3

Allele class
Mutagen	in vitro construct
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct (Wagner et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The EGFP open reading frame (ORF) and a poly(A) site is fused downstream of a fragment consisting of the His3 promoter and the first 62 amino acids of the His3 ORF fused to the 3'UTR of His3 (which includes the stem loop and histone downstream element (HDE) sequences but not the downstream polyadenylation signals). (Wagner et al., 2007) The His3 promoter, amino acids 1-62 of the His3 open reading frame (ORF) and the His3 3' end containing a portion of the downstream intergenic region (from immediately after the stop codon through until 18bp downstream of the HDE) are fused upstream of the EGFP ORF. The His3 ORF is in frame with the EGFP ORF. A single mutation (U to A) has been introduced within the HDE in order to disrupt a stop codon. (Wagner et al., 2007)
Carried in construct	P{His3-SL-HDE-EGFP} (Wagner et al., 2007, Wagner et al., 2007)
Tagged with	T:Avic\GFP^EGFP (Wagner et al., 2007, Wagner et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	P{His3-SL-HDE-EGFP} acts as a reporter of histone pre-mRNA misprocessing; normal histone pre-mRNA processing results in mRNA lacking the Avic\GFP open reading frame, while misprocessing leads to readthrough of transcription and production of mRNA encoding Avic\GFP. (Wagner et al., 2007)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	His3^{SL.HDE.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Wagner et al., 2007, Mol. Cell 28(4): 692--699 A genome-wide RNA interference screen reveals that variant histones are necessary for replication-dependent histone pre-mRNA processing. [FBrf0201575]
Supplementary material	Wagner et al., 2007, Mol. Cell 28(4): Supplemental Data. [FBrf0208092]

Allele Dmel\His3SL.HDE.T:Avic\GFP-EGFP

Allele Dmel\His3^{SL.HDE.T:Avic\GFP-EGFP}