Allele Dmel\TBPH^Δ23

General Information
Symbol	Dmel\TBPH^Δ23	Species	D. melanogaster
Name		FlyBase ID	FBal0221488
Feature type	allele	Associated gene	Dmel\TBPH

Map ( GBrowse )
Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Feiguin et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References deletion 2R:19,748,477..19,750,093 comment=A 1616bp deletion resulting from the excision of P{EPgy2}TBPHEY10530. The P element was completely excised. (Feiguin et al., 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EPgy2}TBPH^EY10530 (Feiguin et al., 2009)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion, resulting in a deletion of 1616bp of genomic DNA with complete elimination of the inserted element. The breakpoints of the deletion are 2R:19748477-19750093. (Feiguin et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pharate adult stage \| recessive (Feiguin et al., 2009) locomotor behavior defective, with Scer\GAL4^elav.PLu, TBPH^GD6943 (Feiguin et al., 2009) locomotor behavior defective (with Df(2R)BSC660) (Wang et al., 2011) locomotor behavior defective \| adult stage \| recessive (Feiguin et al., 2009) neuroanatomy defective \| third instar larval stage (Feiguin et al., 2009) partially lethal - majority die (with Df(2R)BSC660) (Wang et al., 2011) short lived (Feiguin et al., 2009) short lived (with Df(2R)BSC660) (Wang et al., 2011)
Phenotype Manifest In
	embryonic/larval neuromuscular junction (Feiguin et al., 2009) type I bouton \| third instar larval stage (Feiguin et al., 2009)
Detailed Description
	Statement Reference Compared with controls, only about 20% of the expected TBPH[Δ23]/Df(2R)BSC660 mutants survive to adulthood. The escapers do not show any defects in eye, wing or genital development. Compared with controls, TBPH[Δ23]/Df(2R)BSC660 mutants show a dramatic reduction of locomotor speed. Compared with wild-type, the survival of TBPH[Δ23]/Df(2R)BSC660 mutants is markedly reduced. Larval neuromuscular junction synapse morphology appears normal in TBPH[Δ23]/Df(2R)BSC660 mutants. (Wang et al., 2011) Homozygotes survive embryogenesis and more than 60% reach the pupal stage. However, only 21% of homozygotes eclose, with 32% becoming trapped in the pupal case. Homozygous adults that eclose are morphologically normal, but they have dramatic locomotive defects, in both walking and climbing assays. Homozygous adults have a reduced lifespan compared to controls. Flies expressing TBPH[GD6943] under the control of Scer\GAL4[elav.PLu] in a TBPH[Δ23]/+ background show defects in both walking and climbing assays. The number of type 1b and 1s boutons and the number of synaptic branches at the neuromuscular junction of muscles 6 and 7 is reduced in homozygous larvae compared to controls. (Feiguin et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Df(2R)BSC660/TBPH^Δ23 has locomotor behavior defective phenotype, suppressible \| partially by Scer\GAL4^elav-C155/caz^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011) Df(2R)BSC660/TBPH^Δ23 has locomotor behavior defective phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\TARDBP^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011) Df(2R)BSC660/TBPH^Δ23 has partially lethal - majority die phenotype, suppressible by Scer\GAL4^elav-C155/caz^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011) Df(2R)BSC660/TBPH^Δ23 has partially lethal - majority die phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\TARDBP^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011) Df(2R)BSC660/TBPH^Δ23 has short lived phenotype, suppressible by Scer\GAL4^elav-C155/caz^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011) Df(2R)BSC660/TBPH^Δ23 has short lived phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\TARDBP^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011) TBPH^Δ23 has locomotor behavior defective \| recessive \| adult stage phenotype, suppressible by Hsap\TARDBP^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^D42 (Feiguin et al., 2009) TBPH^Δ23 has locomotor behavior defective \| recessive \| adult stage phenotype, suppressible by Hsap\TARDBP^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^elav.PLu (Feiguin et al., 2009) TBPH^Δ23 has short lived phenotype, suppressible \| partially by Hsap\TARDBP^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^D42 (Feiguin et al., 2009)
NOT Enhancer of
Statement Reference Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^Rapgap1-OK6 is a non-enhancer of neuroanatomy defective phenotype of Scer\GAL4^Rapgap1-OK6, caz^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011)
NOT Suppressor of
Statement Reference Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^Rapgap1-OK6 is a non-suppressor of neuroanatomy defective phenotype of Scer\GAL4^Rapgap1-OK6, caz^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference TBPH^Δ23 has embryonic/larval neuromuscular junction phenotype, suppressible by Hsap\TARDBP^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^D42 (Feiguin et al., 2009) TBPH^Δ23 has type I bouton \| third instar larval stage phenotype, suppressible by Hsap\TARDBP^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^D42 (Feiguin et al., 2009)
NOT Enhancer of
Statement Reference Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^Rapgap1-OK6 is a non-enhancer of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Scer\GAL4^Rapgap1-OK6, caz^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011)
NOT Suppressor of
Statement Reference Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^Rapgap1-OK6 is a non-suppressor of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Scer\GAL4^Rapgap1-OK6, caz^{Scer\UAS.x10.T:Zzzz\FLAG} (Wang et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Neuronal overexpression of caz[Scer\UAS.x10.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] in TBPH[Δ23]/Df(2R)BSC660 mutants restores their eclosion rate and longevity to levels not significantly different from that of wild-type animals. Although overexpression of caz[Scer\UAS.x10.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] in TBPH[Δ23]/Df(2R)BSC660 mutants significantly improves their locomotion velocity, these animals are still significantly slower than controls. The level of NMJ expansion induced by the overexpression of caz[Scer\UAS.x10.T:Zzzz\FLAG] via Scer\GAL4[Rapgap1-OK6] is not affected by TBPH[Δ23]/Df(2R)BSC660. (Wang et al., 2011)
Xenogenetic Interactions
Statement Reference Neuronal expression of Hsap\TARDBP[Scer\UAS.x10.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] completely suppresses the locomotory, life span and viability defects in TBPH[Δ23]/Df(2R)BSC660 mutants. (Wang et al., 2011) Expression of Hsap\TARDBP[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[D42] partially rescues the reduced lifespan of TBPH[Δ23] flies. Expression of Hsap\TARDBP[Scer\UAS.T:Zzzz\FLAG] under the control of either Scer\GAL4[elav.PLu] or Scer\GAL4[D42] rescues the walking and climbing defects of TBPH[Δ23] flies. The reduced number of synaptic branches and of type 1s and 1b boutons which are seen at the neuromuscular junction of muscles 6 and 7 in TBPH[Δ23] larvae are rescued by expression of Hsap\TARDBP[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[D42]. (Feiguin et al., 2009)
Complementation & Rescue Data
Rescued by	Df(2R)BSC660/TBPH^Δ23 is rescued by TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011)
Partially rescued by	TBPH^Δ23 is partially rescued by TBPH^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^D42 (Feiguin et al., 2009)
Comments	Neuronal expression of TBPH[Scer\UAS.10x.T:Avic\GFP-YFP.Venus] under the control of Scer\GAL4[elav-C155] fully rescues the locomotory defects, reduced viability and longevity phenotypes of TBPH[Δ23]/Df(2R)BSC660 mutants. (Wang et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	tbph^Δ23 (Wang et al., 2011) TBPH^Δ23 (Feiguin et al., 2009) Δ23 (Hazelett et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Hazelett et al., 2012, G3 (Bethesda) 2(7): 789--802 Comparison of Parallel High-Throughput RNA Sequencing Between Knockout of TDP-43 and Its Overexpression Reveals Primarily Nonreciprocal and Nonoverlapping Gene Expression Changes in the Central Nervous System of Drosophila. [FBrf0219102] Wang et al., 2011, J. Clin. Invest. 121(10): 4118--4126 The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span. [FBrf0216240] Feiguin et al., 2009, FEBS Lett. 583(10): 1586--1592 Depletion of TDP-43 affects Drosophila motoneurons terminal synapsis and locomotive behavior. [FBrf0208082]

Allele Dmel\TBPHΔ23

Allele Dmel\TBPH^Δ23