Allele Dmel\Manf^Δ96

General Information
Symbol	Dmel\Manf^Δ96	Species	D. melanogaster
Name		FlyBase ID	FBal0221493
Feature type	allele	Associated gene	Dmel\Manf

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{SUPor-P}CG14879^KG03250 (Palgi et al., 2009)
Nature of the lesion	Statement Reference Imprecise excision of the P{SUPor-P}CG14879[KG03250] insertion, resulting in a deletion that removes 278bp of the Manf open reading frame, including the first 2 exons and part of the third exon. (Palgi et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death \| embryonic stage 17 \| maternal effect \| recessive (Palgi et al., 2009) lethal \| embryonic stage \| maternal effect \| recessive (Palgi et al., 2009) lethal \| larval stage \| recessive (Palgi et al., 2009) locomotor behavior defective \| larval stage \| recessive (Palgi et al., 2009)
Phenotype Manifest In
	embryonic/first instar larval cuticle \| maternal effect (Palgi et al., 2009) embryonic/larval cell body glial cell \| embryonic stage 17 \| maternal effect (Palgi et al., 2009) neurite & dopaminergic neuron \| larval stage (Palgi et al., 2009) neuropil \| embryonic stage 17 \| maternal effect (Palgi et al., 2009) ventral nerve cord \| embryonic stage 17 \| maternal effect (Palgi et al., 2009)
Detailed Description
	Statement Reference Homozygotes die as later first instar larvae or immediately after the delayed first moult with defects in behaviour; the larvae atypically wander away from the food, then move more slowly and finally freeze, immobilised but still responding to touch. Embryonic neural development occurs normally in homozygotes. The volume of DA neurites is significantly diminished in homozygous larvae, whereas the neuronal somae persist. The neuritic degeneration occurs mostly in the abdominal area of the ventral nerve cord. There is no significant change in the neurite volume of serotonergic neurons or in the volume of the motorneuron subpopulation in these larvae. Manf[Δ96] embryos lacking both maternal and zygotic Manf[+] function develop normally until late stage 16, but show severe defects thereafter; the ventral nerve cord (VNC) becomes overcondensed compared to wild type. At early stage 16, the neuropile of the VNC still resembles that of wild type, but by late stage 17, degradation of axonal membranes is seen, starting at the border of the glia and neuropile. In addition, cell body glial cells appear highly electron dense and contain remnants of cell debris. In the VNC, dying cells with poor and bleached cytoplasm, swollen nuclei and with dilated endoplasmic reticulum and other organelles are seen, which is atypical for conventional apoptosis. The cuticle of these embryos is defective; all cuticular layers are disorganised. (Palgi et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Manf^Δ96 has lethal \| recessive \| larval stage phenotype, suppressible by Hsap\MANF^Scer\UAS.cPa/Scer\GAL4^da.G32 (Palgi et al., 2009) Manf^Δ96 has lethal \| recessive \| larval stage phenotype, suppressible by Hsap\MANF^Scer\UAS.cPa/Scer\GAL4^tub.PU (Palgi et al., 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\MANF[Scer\UAS.cPa] under the control of one of Scer\GAL4[da.G32] or Scer\GAL4[tub] significantly rescues the larval lethality of Manf[Δ96] animals (assayed by survival into the pupal stage). (Palgi et al., 2009)
Complementation & Rescue Data
Rescued by	Manf^Δ96 is rescued by Manf^Scer\UAS.cPa/Scer\GAL4^69B (Palgi et al., 2009) Manf^Δ96 is rescued by Scer\GAL4^da.G32/Manf^Scer\UAS.cPa (Palgi et al., 2009) Manf^Δ96 is rescued by Scer\GAL4^tub.PU/Manf^Scer\UAS.cPa (Palgi et al., 2009)
Not rescued by	Manf^Δ96 is not rescued by Manf^Scer\UAS.cPa/Scer\GAL4^c135 (Palgi et al., 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	DmMANF^Δ96 (Palgi et al., 2009) Manf^Δ96
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Palgi et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(7): 2429--2434 Evidence that DmMANF is an invertebrate neurotrophic factor supporting dopaminergic neurons. [FBrf0206690]

Allele Dmel\ManfΔ96

Allele Dmel\Manf^Δ96