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General Information
Symbol
Dmel\Atg1UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0221500
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Myc-Atg1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the Atg1 coding region, which is tagged with six copies of Tag:MYC.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PU results in the accumulation of acidic vesicles in most of the eye imaginal disc cells posterior to the morphogenetic furrow. There is also ectopic cell death in this area of the eye imaginal disc.

Expression of Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.PB using a 1 hour heat shock results in a weak induction of autophagy; a few, small autophagosomes are seen, mostly confined to the perinuclear region.

Expression of Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP in fat body cell clones has no appreciable effect on cell size.

Expression of Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF has no discernible effect on eye development.

Expression of Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Cg.PA does not affect larval growth or developmental timing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Suppressor of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU at 18[o]C results in reduced viability and fertility, as well as eye degeneration. Raising the flies at 18[o]C restores fertility, whilst maintaining the eye degeneration. Retinal cells such as photoreceptors and cone cells exhibit degeneration and there is complete disarray of the ommatidia structure and accumulation of membrane-dense vacuoles. After 4 weeks the retinal structure has completely degenerated except for the corneal lens exocuticle. Prominent cell death is seen in the differentiation ommatidia in eye disc and autophagic flux is increased.

Expression of GyfHM05106 dramatically suppresses the eye degeneration and eye disc cell death seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C. However, the photoreceptor morphology is not fully restored. The increased autophagic flux in eye discs is also suppressed.

Expression of Atg1JF02273 completely suppresses the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.

Expression of 4EHPGL01035 does not suppress the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.

Expression of picoGD7437 does not suppress the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.

Co-expression of Atg17HMS01611 almost completely suppresses the accumulation of acidic vesicles and increased cell death seen posterior to the morphogenetic furrow in the eye discs of larvae expressing Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PU.

Co-expression of Atg13Scer\UAS.cCa results in a marked increase in the number, size, and distribution of autophagosomes in animals expressing Atg1Scer\UAS.T:Hsap\MYC under the control of low levels of Scer\GAL4hs.PB (using a 1 hour heat shock).

Autophagy is no longer induced by expression of Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Cg.PA in clones of cells that are also homozygous for Atg13Δ74.

Co-expression of Atg13Scer\UAS.cCa and Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP in fat body cell clones results in cells that are markedly reduced in size compared to neighbouring control cells.

Co-expression of Atg13Scer\UAS.cCa and Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF leads to disruption of ommatidial patterning.

Expression of Atg1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.PB can partially rescue the lethality, but not the autophagy defect, of Atg13unspecified homozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Atg1Scer\UAS.T:Hsap\MYC
Atg1UAS.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (5)