FlyBase Allele Report: Dmel\Atg13[Δ81]

General Information

Symbol

Dmel\Atg13^Δ81

Species

D. melanogaster

Name

FlyBase ID

FBal0221508

Feature type

allele

Associated gene

Dmel\Atg13

Associated Insertion(s)

Carried in Construct

Also Known As

atg13⁸¹

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chang and Neufeld, 2009)

Mutagen

P-element activity

(Chang and Neufeld, 2009)

Progenitor genotype

P{GSV6}Atg13^GS11822

(Chang and Neufeld, 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes 593bp including the translation start site and the first 104 codons of Atg13.

(Chang and Neufeld, 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by scrib⁶⁷³, Ras85D^V12.UAS

(Katheder et al., 2017)

modeled by Ras85D^V12.cUa.UAS, scrib¹

(Khezri et al., 2021)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^695.UAS

(Zhuang et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Atg13^Δ81 homozygous clones in mosaic third instar larval eye-antennal imaginal discs occupy a significantly smaller proportion of the disc area and result in decreased eye sizes in adults, as compared to control clones.

(Katheder et al., 2017)

Third larval instar eye-antennal discs have normal morphology.

(Morelli et al., 2014)

The kinetics of the clearance of the paternal mitochondrial derivatives is normal in embryos derived from females carrying Atg13^Δ81 germline clones.

(Politi et al., 2014)

Atg13^Δ81 mutants display basal autophagy defects in larval fat body cells.

(Pircs et al., 2012)

Heat-shock induced Atg13^Δ81 follicle cell clones generate eggs where approximately 42% exhibit dorsal appendage defects and 34% hatch.

Atg13^Δ74/Atg13^Δ81 mutant mosaic germline cells are defective in autophagy (as monitored by the lack of lysotracker staining). However, the chimeras are fully fertile with normal egg-laying behavior and hatching rates. There are no defects in egg chamber development or egg morphology. These germline mosaics do not show disruption of nurse cell apoptosis.

(Barth et al., 2011)

Females carrying homozygous germline clones have a significant increase in the number of stage 14 egg chambers that have persisting TUNEL-negative nurse cell nuclei compared to wild-type egg chambers at the same stage, in which nurse cell nuclei are rarely detected (and those that are detected are all TUNEL positive). The persisting nurse cell nuclei show condensed nuclear staining.

(Nezis et al., 2010)

Homozygous clones in the larval fat body fail to induce autophagy in response to treatment with rapamycin.

(Chang and Neufeld, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by Atg14^EY14568/Atg14^Δ5.2

(Katheder et al., 2017)

Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by Atg8a^d4/Atg8a^d4

(Katheder et al., 2017)

Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has abnormal size | somatic clone | third instar larval stage phenotype, suppressible by Atg14^EY14568/Atg14^Δ5.2

(Katheder et al., 2017)

Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has abnormal size | somatic clone | third instar larval stage phenotype, suppressible by Atg8a^d4/Atg8a^d4

(Katheder et al., 2017)

Suppressor of

Statement

Reference

(Katheder et al., 2017)

Atg13^{+tCH322-168O18}, Atg13^Δ81/Atg13^Δ81 is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81, Atg13^Δ81, Atg14^Δ5.2, Atg14^Δ5.2 is a suppressor | partially | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^ey.3.5, Atg13^Δ81/Atg13^Δ81 is a suppressor | partially of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

(Katheder et al., 2017)

Atg13^{+tCH322-168O18}, Atg13^Δ81/Atg13^Δ81 is a suppressor of abnormal size | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81, Atg13^Δ81, Atg14^Δ5.2, Atg14^Δ5.2 is a suppressor | partially | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^ey.3.5, Atg13^Δ81/Atg13^Δ81 is a suppressor | partially of abnormal size | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a suppressor | cell non-autonomous of increased cell number | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

NOT Suppressor of

Statement

Reference

Atg13^Δ81/Atg13^Δ81 is a non-suppressor | cell non-autonomous of abnormal size | somatic clone | third instar larval stage phenotype of Scer\GAL4^Act.PU, yki^{S168A.UAS.EGFP,Tag:HA}

(Katheder et al., 2017)

(Katheder et al., 2017)

Atg13^{+tCH322-168O18}, Atg13^Δ81/Atg13^Δ81 is a non-suppressor of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

(Katheder et al., 2017)

Atg13^{+tCH322-168O18}, Atg13^Δ81/Atg13^Δ81 is a non-suppressor of abnormal size | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a non-suppressor | cell autonomous | cell non-autonomous of increased cell death | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a non-suppressor | cell non-autonomous of neoplasia | somatic clone | third instar larval stage phenotype of Scer\GAL4^Act.PU, yki^{S168A.UAS.EGFP,Tag:HA}

(Katheder et al., 2017)

Other

Statement

Reference

Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has abnormal size | somatic clone | third instar larval stage phenotype

(Katheder et al., 2017)

Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has decreased cell number | somatic clone | third instar larval stage phenotype

(Katheder et al., 2017)

Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | third instar larval stage phenotype

(Katheder et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

(Katheder et al., 2017)

Atg13^{+tCH322-168O18}, Atg13^Δ81/Atg13^Δ81 is a suppressor of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81, Atg13^Δ81, Atg14^Δ5.2, Atg14^Δ5.2 is a suppressor | partially | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^ey.3.5, Atg13^Δ81/Atg13^Δ81 is a suppressor | partially of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a suppressor | partially of eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a suppressor of autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a suppressor of larval somatic muscle cell | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a suppressor of embryonic/larval midgut | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a suppressor of embryonic/larval fat body | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81 is a suppressor of autophagosome | somatic clone | larval stage phenotype of Aduk^UAS.cBa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Braden and Neufeld, 2016)

Atg13^Δ81 is a suppressor of embryonic/larval fat body | somatic clone | larval stage phenotype of Aduk^UAS.cBa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Braden and Neufeld, 2016)

NOT Suppressor of

Statement

Reference

(Katheder et al., 2017)

Atg13^{+tCH322-168O18}, Atg13^Δ81/Atg13^Δ81 is a non-suppressor of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^{+tCH322-168O18}, Atg13^Δ81/Atg13^Δ81 is a non-suppressor of eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81/Atg13^Δ81 is a non-suppressor of embryonic/larval fat body | somatic clone phenotype of Scer\GAL4^Act.PU, bsk^UAS.cBa

(Chang and Neufeld, 2009)

Other

Statement

Reference

Atg13^Δ81/Atg13[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing eye-antennal imaginal disc clone tumors are significantly suppressed by Atg13^Δ81 homozygosity in clone cells (leading to reductions in the proportions of clone cells entering the cell cycle, but not undergoing mitosis or apoptosis, and leading to a reduction in the tumor size, but not invasiveness), in neighboring disc cells (leading to reductions in the tumor size and in the proportion of cell entering the cell cycle, but not undergoing mitosis or apoptosis), in the full organism except the clone cells (leading to reductions in the tumor size and invasiveness), or in the full organism (leading to reductions in the proportions of cells entering the cell cycle, undergoing mitosis, but not undergoing apoptosis, and leading to reductions in the tumor size and invasiveness); these scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing tumors are also significantly suppressed by the combination of Atg13^Δ81 homozygosity in the tumor cells and Atg14^Δ5.2 homozygosity in the neighboring disc cells. One copy of Atg13^{+tCH322-168O18} in the full organism suppresses the decrease in tumor size, but not the decrease in invasiveness, resulting from Atg13^Δ81 homozygosity either in the full organism or in the full organism apart from clone cells; one copy of Atg13^ey.3.5 in the full organism also partially suppresses the decrease in tumor size resulting from Atg13^Δ81 homozygosity. These scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing tumors grow to significantly smaller sizes when grafted into the abdomen of either Atg14^Δ5.2/Atg14^EY14568 transheterozygous adult hosts (but not if hosts also possess one copy of Atg14^{+tCH322-175F03}) or Atg8a^d4 homozygous adult hosts, as compared to control hosts.

The cell non-autonomous increase in autophagosomes resulting from the presence of clones homozygous for scrib⁶⁷³ and expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU in third instar larval eye-antennal imaginal discs is suppressed by Atg13^Δ81 homozygosity in either the full organism (leading to suppression in distant tissues - somatic muscles, midgut and fat body) or in the full organism apart from the clone cells (leading to suppression in neighboring disc tissue).

Atg13^Δ81 homozygosity in neighboring disc tissue does not significantly change the size of third instar larval eye-antennal imaginal disc tumors resulting from the clonal expression of yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} under the control of Scer\GAL4^Act.PU.

(Katheder et al., 2017)

The robust formation of Atg8a-positive (or LysoTracker positive) autophagosome vesicles the Aduk^Scer\UAS.cBa-expressing (controlled by Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) fat body clones in fed larvae is not observed when the clones are induced in Atg13^Δ81 mutant background.

(Braden and Neufeld, 2016)

The autophagy that is induced by expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^Act in clones in the larval fat body still occurs in an Atg13^Δ81 homozygous background.

(Chang and Neufeld, 2009)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} under the Scer\GAL4^shot-OK307 driver in adults heterozygous for Atg13^Δ81 has no phenotype, unless the animals are also heterozygous for htt^98E2, in which case a severe loss of thoracic somatic muscles is observed.

(Rui et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Atg13^Δ81

(Zhuang et al., 2020, Billes et al., 2018, Braden and Neufeld, 2016, Morelli et al., 2014, Pircs et al., 2012, Barth et al., 2011, Chang and Neufeld, 2009)

Atg13[d81]

(Nagy et al., 2015)

atg13(Δ81)

(Tyra et al., 2020)

atg13⁸¹

(Tyra et al., 2020, Mukherjee et al., 2016)

atg13^D81

(Jain et al., 2015)

atg13^Δ81

(Khezri et al., 2021, Katheder et al., 2017, Tusco et al., 2017, Jain et al., 2015, Rui et al., 2015, Politi et al., 2014, Nezis et al., 2010)

Δ81

(Chang and Neufeld, 2009)

Name Synonyms

Secondary FlyBase IDs

References (18)

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