FB2025_01 , released February 20, 2025
Allele: Dmel\TimpUAS.cSa
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General Information
Symbol
Dmel\TimpUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Srivastava
FlyBase ID
FBal0221512
Feature type
allele
Associated gene
Dmel\Timp
Associated Insertion(s)
Carried in Construct
P{UAS-Timp.S}
Also Known As
UAS-TIMP
Key Links
Transgenic product class
wild_type
(Srivastava et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Srivastava et al., 2007)
Mutagen
in vitro construct
(Srivastava et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-Timp.S}
Cytology
Description

UAS regulatory sequences drive expression of Timp.

(Srivastava et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Timp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  carcinoma
      modeled by Ras85DV12.UAS, scrib1
      (Srivastava et al., 2007)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of TimpScer\UAS.cSa under the control of Scer\GAL4Lsp2.PH results in a thoracic defects, ranging from clefts in the notum at the dorsal midline to a thorax missing one or both heminota (the heminotum remains inside the animal together with the corresponding wing).

      85% of pupae expressing TimpScer\UAS.cSa under the control of Scer\GAL4Lsp2.PH show wing eversion defects 6 hours after puparium formation (eversion is complete at this time in wild-type animals); 23% have partially everted wings and 62% show a complete failure of disc eversion. 12% have leg eversion defects.

      98.1% of pupae expressing TimpScer\UAS.cSa under the control of Scer\GAL4Ubx-lac1-Gal4 show wing eversion defects 6 hours after puparium formation (eversion is complete at this time in wild-type animals); 5.1% have partially everted wings and 92.9% show a complete failure of disc eversion.

      The basement membrane of wing discs is not degraded in pupae expressing TimpScer\UAS.cSa under the control of either Scer\GAL4Lsp2.PH or Scer\GAL4Ubx-lac1-Gal4 which fail to evert their wing discs.

      (Srivastava et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos expressing TimpScer\UAS.cSa and ReckScer\UAS.cSa in the haemocytes under the control of Scer\GAL4srp.Hemo display normal haemocyte migration into the tail region of the embryo.

      (Siekhaus et al., 2010)

      The degradation of the wing disc basement membrane that is seen in animals expressing Tak1Scer\UAS.cMa under the control of Scer\GAL4ptc-559.1 is suppressed by co-expression of TimpScer\UAS.cSa.

      The invasive behaviour and degradation of the basement membrane of clones in the eye antennal disc which are expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C and are also homozygous for scrib1 is suppressed if the clones are also co-expressing both ReckScer\UAS.cSa and TimpScer\UAS.cSa simultaneously.

      (Srivastava et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TimpScer\UAS.cSa
      TimpUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Srivastava
      Secondary FlyBase IDs
        References (3)