FB2025_01 , released February 20, 2025
Allele: Dmel\BHDRNAi.Sym.UAS
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General Information
Symbol
Dmel\BHDRNAi.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0221543
Feature type
allele
Associated gene
Dmel\BHD
Associated Insertion(s)
Carried in Construct
P{Sym-UAS-BHD}
Key Links
Transgenic product class
RNAi_reagent
(Singh et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Singh et al., 2006)
Mutagen
in vitro construct
(Singh et al., 2006)
Progenitor genotype
Carried in construct
P{Sym-UAS-BHD}
Cytology
Description

Sense-antisense transcription of a BHD cDNA (driven by two convergent arrays of UAS regulatory sequences) results in double stranded RNA (dsRNA) expression.

(Singh et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
BHD
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of BHDdsRNA.Sym.Scer\UAS under the control of Scer\GAL4tub.PU results in an increase in nucleolar volume in larval salivary gland cells. In addition, the nucleoli are multi-lobed (in contrast to the round nucleolus seen in wild type).

      Adults expressing BHDdsRNA.Sym.Scer\UAS under the control of Scer\GAL4tub.PU are larger in size and weigh more than wild-type controls.

      (Gaur et al., 2013)

      Males expressing BHDdsRNA.Sym.Scer\UAS under the control of Scer\GAL4nos.PG have reduced fertility. The males show a significant reduction in the number of germline stem cells (average of 3.70 per testis) compared to controls (average of 7.91 per testis). The mutant males also show loss of spermatogonia, as evidenced by holes in the location where they are normally found. In addition, a significant increase in dying cell clusters at the spermatogonial location is seen in the mutant testes.

      Males expressing P{Sym-UAS-BHD} (which carries both BHDSym.Scer\UAS and BHDa.Sym.Scer\UAS) under the control of Scer\GAL4nos.PG have reduced fertility. The males show a significant reduction in the number of germline stem cells (average of 3.70 per testis) compared to controls (average of 7.91 per testis). The mutant males also show loss of spermatogonia, as evidenced by holes in the location where they are normally found. In addition, a significant increase in dying cell clusters at the spermatogonial location is seen in the mutant testes.

      (Singh et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of BHDdsRNA.Sym.Scer\UAS enhances the semi-lethality caused by expression of Ras85DV12.Scer\UAS under the control of Scer\GAL4ey.PU at 21-22[o]C, such that few adult escapers emerge. The eye defects of adult escapers are enhanced.

      Co-expression of BHDdsRNA.Sym.Scer\UAS exacerbates the increase in the number of mitotic cells per eye imaginal disc seen in larvae expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4ey.PU.

      (Gaur et al., 2013)

      Co-expression of BHDdsRNA.Sym.Scer\UAS significantly suppresses the testis phenotype caused by expression of upd1Scer\UAS.cCa under the control of Scer\GAL4nos.PG; the number of germline stem cells is significantly reduced, and the overall shape of the testis is closer to that of wild type.

      Co-expression of BHDdsRNA.Sym.Scer\UAS significantly suppresses the male germline stem cell expansion phenotype caused by expression of dppScer\UAS.cUa under the control of Scer\GAL4nos.PG.

      Co-expression of P{Sym-UAS-BHD} (which carries both BHDSym.Scer\UAS and BHDa.Sym.Scer\UAS) significantly suppresses the testis phenotype caused by expression of osScer\UAS.cCa under the control of Scer\GAL4nos.PG; the number of germline stem cells is significantly reduced, and the overall shape of the testis is closer to that of wild type.

      Co-expression of P{Sym-UAS-BHD} (which carries both BHDSym.Scer\UAS and BHDa.Sym.Scer\UAS) significantly suppresses the male germline stem cell expansion phenotype caused by expression of dppScer\UAS.cUa under the control of Scer\GAL4nos.PG.

      (Singh et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      BHDRNAi.Sym.UAS is rescued by Scer\GAL4Tub.PU/BHDUAS.Tag:HA

      (Gaur et al., 2013)
      Comments

      Co-expression of BHDScer\UAS.T:Ivir\HA1 suppresses the increase in nucleolar volume seen in salivary gland cells of larvae expressing BHDdsRNA.Sym.Scer\UAS under the control of Scer\GAL4tub.PU.

      (Gaur et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Associated with: BHDa.Sym.Scer\UAS.

      (Singh et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      BHDRNAi.Sym.UAS
      BHDSym.Scer\UAS
      BHDdsRNA.Sym.Scer\UAS
      BHDdsRNA.Sym.UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0221544
      References (3)