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General Information
Symbol
Dmel\Hus1-like37
Species
D. melanogaster
Name
FlyBase ID
FBal0221553
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hus137
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

3297bp deletion resulting from the imprecise excision of P{GT1}BG00590.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mobilisation of P{GT1}BG00590, which is inserted 2kb downstream of Hus1-like, results in a 3297 base deletion, which removes the entire Hus1-like ORF but does not delete any other transcript.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous Hus1-like37 flies show normal locomotor activity rhythms.

Mutant larvae show cell cycle arrest after a 500Rad irradiation exposure. They also show a normal reduction in BrdU incorporation after a 4000Rad irradiation exposure.

Hus1-like37 flies are viable although female sterile.

Hus1-like37 mutant flies are sensitive to hydroxyurea and methyl methanesulfonate (MMS) but not to X-rays (2500 rad). Exposure to 10 or 20 mM hydroxyurea affects the survival of Hus1-like37 mutants, whereas treatment with 30 mM hydroxyurea eliminates almost all Hus1-like37 and Hus1-like37/Df(3R)110 mutant larvae. Relatively low doses of MMS (0.025%) causes almost 100% death of Hus1-like37 and Hus1-like37/Df(3R)110 mutant larvae. Most of the Hus1-like37mutants die as larvae. When Hus1-like37 homozygous first and early second instar larvae are separated from their heterozygous siblings before MMS treatment, only 19% survive to pupal stage, whereas 75% of their heterozygous siblings form pupae. For both genotypes around20% die as pharate adults.

Approximately 15.% of Hus1-like37 mutant larvae treated with 0.025% methyl methanesulfonate exhibit aneuploid nuclei, an approximately four-fold increase as compared with wild-type.

Treatment of Hus1-like37 mutant flies with 4000 rads of irradiation does not result in a decresase of homozygous flies relative to untreated controls. Similar to wild-type, Hus1-like37 mutant discs contain very few mitotic cells after irradiation. Four hours after irradiation, Hus1-like37 mutant discs exhibit wild-type levels of apoptosis, indicating that Hus1-like is not required for post-irradiation induction of apoptosis.

Approximately 92% of eggs laid by Hus1-like37 mutant mothers exhibit nuclear defects, where the nucleus is found in a variety of conformations including the smooth spherical wild-type shape, oblong shape, or in several separate pieces along the nuclear periphery. Similar defects are generated from Hus1-like37/Df(3R)110 mothers.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference

Hus1-like37/Hus1-like[+] is a suppressor | partially of dorsal appendage phenotype of spn-BBU

Hus1-like37/Hus1-like[+] is a suppressor | partially of egg phenotype of spn-BBU

Hus1-like37 is a suppressor of nucleus phenotype of okrAA

Hus1-like37 is a suppressor of egg phenotype of okrAA

Hus1-like37 is a suppressor of oocyte phenotype of okrAA

Hus1-like37 is a suppressor of dorsal appendage phenotype of okrAA

Hus1-like37/Hus1-like[+] is a suppressor | partially of karyosome phenotype of spn-BBU

Hus1-like37 is a suppressor of dorsal appendage phenotype of spn-BBU

Hus1-like37 is a suppressor of egg phenotype of spn-BBU

Hus1-like37 is a suppressor of karyosome phenotype of spn-BBU

Hus1-like37/Hus1-like[+] is a suppressor | partially of nucleus phenotype of okrAA

Hus1-like37/Hus1-like[+] is a suppressor | partially of egg phenotype of okrAA

Hus1-like37/Hus1-like[+] is a suppressor | partially of oocyte phenotype of okrAA

Hus1-like37/Hus1-like[+] is a suppressor | partially of dorsal appendage phenotype of okrAA

NOT Suppressor of
Statement
Reference

Hus1-like37/Hus1-like[+] is a non-suppressor of karyosome phenotype of okrAA

Hus1-like37 is a non-suppressor of karyosome phenotype of okrAA

Additional Comments
Genetic Interactions
Statement
Reference

A heterozygous Hus1-like37 background partially suppresses the abnormal egg phenotype for eggs laid by spn-BBU homozygous mutant mothers, with only 45% of eggs exhibiting partially or completely fused appendages or lacking appendages altogether, compared to 51% in okrAA mutants.

A homozygous Hus1-like37 background completely suppresses the abnormal egg phenotype for eggs laid by spn-BBU homozygous mutant mothers.

A heterozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with spn-BBU homozygous mutants.

A homozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with spn-BBU homozygous mutants.

A heterozygous Hus1-like37 background partially suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers, with only 35% of eggs exhibiting partially or completely fused appendages or lacking appendages altogether, compared to 51% in okrAA mutants.

A homozygous Hus1-like37 background completely suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers.

A heterozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

A homozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Hus1-likefl.Scer\UAS under the control of Scer\GAL4Act5C rescues the karyosome defects associated with Hus1-like37 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)