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General Information
Symbol
Dmel\RagA-BQ61L.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0221638
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-RagAQ61L
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: Q61L.
UASt regulatory sequences drive expression of a constitutively active form of RagA-B.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Ectopic expression of RagA-BQ61L.Scer\UAS under the control of the Scer\GAL4elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.
Expression of RagAQ61L.Scer\UAS using Scer\GAL4nub-AC-62 results in abnormally large wings in which cell number is increased.
Expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4en.PU results in an increase in the area of the posterior compartment of the wing (the domain of driver expression) compared to the control anterior compartment.
Expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4en-e16E results in a significant increase in the size of the posterior compartment of the wing, compared with that of the control anterior compartment of the wing. The average size of individual cells in the posterior compartment of the wing is significantly increased in these flies. Under starvation conditions, expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4unspecified in clones in the larval fat body results in a 3-fold increase in cell size compared with neighbouring control cells. Under normal fed conditions, expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4unspecified in clones in the larval fat body has little effect on cell size. The number of autophagosomes per cell is reduced in clones of fat body cells expressing RagAQ61L.Scer\UAS under the control of Scer\GAL4unspecified under starvation conditions compared to control cells under these conditions. Expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4Cg.PA has no significant effect on viability under normal fed conditions, but results in a significantly accelerated death rate under starvation conditions compared to control flies.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Ectopic expression of RagA-BQ61L.Scer\UAS under the control of the Scer\GAL4elav-C155 driver fully restores the decreased number of neuromuscular junction boutons characteristic for the Trpml1 mutant third instar larvae. Ectopic expression of RagA-BQ61L.Scer\UAS under the control of the Scer\GAL4elav-C155 driver in the Trpml1 mutant background significantly increases the proportion of adults that eclose from pupal cases compared to the Trpml1 mutants. Ectopic expression of RagA-BQ61L.Scer\UAS under the control of the Scer\GAL4elav-C155 driver fully restores the decreased number of neuromuscular junction boutons characteristic for the cln3ΔMB1 mutant third instar larvae.
Expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4Cg.PA suppresses the increase in Lysotracker-positive vesicle volume seen in trpml1 mutant fat body cells.
Co-expression of hppyScer\UAS.cMa cancels the increase in wing size and wing cell number resulting from expression of RagA[Q61L.Scer\UAS] using Scer\GAL4nub-AC-62.
The overgrowth of the posterior compartment of the wing which is caused by expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4en.PU is still seen in a hppySH1261 background.
The decrease in relative cell area compared to controls that is seen in Rheb26.2 clones in the fat body is not rescued by expression of RagAQ61L.Scer\UAS under the control of Scer\GAL4unspecified.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
RagA-BQ61L.Scer\UAS
RagA-BQ61L.UAS
RagAQ61L.Scer\UAS
dRagAQ61L
Name Synonyms
Secondary FlyBase IDs
    References (7)