Ectopic expression of
RagA-BQ61L.Scer\UAS under the control of the
Scer\GAL4elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.
Expression of
RagAQ61L.Scer\UAS under the control of
Scer\GAL4en.PU results in an increase in the area of the posterior compartment of the wing (the domain of driver expression) compared to the control anterior compartment.
Expression of
RagAQ61L.Scer\UAS under the control of
Scer\GAL4en-e16E results in a significant increase in the size of the posterior compartment of the wing, compared with that of the control anterior compartment of the wing. The average size of individual cells in the posterior compartment of the wing is significantly increased in these flies.
Under starvation conditions, expression of
RagAQ61L.Scer\UAS under the control of
Scer\GAL4unspecified in clones in the larval fat body results in a 3-fold increase in cell size compared with neighbouring control cells. Under normal fed conditions, expression of
RagAQ61L.Scer\UAS under the control of
Scer\GAL4unspecified in clones in the larval fat body has little effect on cell size.
The number of autophagosomes per cell is reduced in clones of fat body cells expressing
RagAQ61L.Scer\UAS under the control of
Scer\GAL4unspecified under starvation conditions compared to control cells under these conditions.
Expression of
RagAQ61L.Scer\UAS under the control of
Scer\GAL4Cg.PA has no significant effect on viability under normal fed conditions, but results in a significantly accelerated death rate under starvation conditions compared to control flies.