FlyBase Allele Report: Dmel\RagA-B[Q61L.UAS]

General Information

Symbol

Dmel\RagA-B^Q61L.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0221638

Feature type

allele

Associated gene

Dmel\RagA-B

Associated Insertion(s)

Carried in Construct

P{UAS-RagA-B.Q61L}

Also Known As

UAS-RagA^Q61L

Key Links

Transgenic product class

missense_variant

(Kim et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kim et al., 2008)

Mutagen

in vitro construct

(Kim et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-RagA-B.Q61L}

Cytology

Description

Amino acid replacement: Q61L.

(Kim et al., 2008)

UASt regulatory sequences drive expression of a constitutively active form of RagA-B.

(Kim et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

RagA-B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

abnormal stress response, with Scer\GAL4^Cg.PA

(Kim et al., 2008)

increased cell number, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

increased cell size, with Scer\GAL4^en-e16E

(Kim et al., 2008)

visible, with Scer\GAL4^en.PU

(Bryk et al., 2010)

Phenotype Manifest In

autophagosome, with Scer\GAL4^unspecified

(Kim et al., 2008)

wing, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

wing blade posterior compartment, with Scer\GAL4^en.PU

(Bryk et al., 2010)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Kim et al., 2008)

Detailed Description

Statement

Reference

Ectopic expression of RagA-B^{Q61L.Scer\UAS} under the control of the Scer\GAL4^elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.

(Wong et al., 2015)

Expression of RagA^{Q61L.Scer\UAS} using Scer\GAL4^nub-AC-62 results in abnormally large wings in which cell number is increased.

(Resnik-Docampo and de Celis, 2011)

Expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^en.PU results in an increase in the area of the posterior compartment of the wing (the domain of driver expression) compared to the control anterior compartment.

(Bryk et al., 2010)

Expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^en-e16E results in a significant increase in the size of the posterior compartment of the wing, compared with that of the control anterior compartment of the wing. The average size of individual cells in the posterior compartment of the wing is significantly increased in these flies.

Under starvation conditions, expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^unspecified in clones in the larval fat body results in a 3-fold increase in cell size compared with neighbouring control cells. Under normal fed conditions, expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^unspecified in clones in the larval fat body has little effect on cell size.

The number of autophagosomes per cell is reduced in clones of fat body cells expressing RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^unspecified under starvation conditions compared to control cells under these conditions.

Expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^Cg.PA has no significant effect on viability under normal fed conditions, but results in a significantly accelerated death rate under starvation conditions compared to control flies.

(Kim et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RagA-B^Q61L.UAS, Scer\GAL4^nub-AC-62 has abnormal size phenotype, suppressible by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

RagA-B^Q61L.UAS, Scer\GAL4^nub-AC-62 has increased cell number phenotype, suppressible by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

NOT Enhancer of

Statement

Reference

RagA-B^Q61L.UAS/Scer\GAL4^pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^nSyb.PS/RagA-B^Q61L.UAS is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Cln3^ΔMB1

(Wong et al., 2015)

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of partially lethal - majority die phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of some die during pharate adult stage phenotype of Trpml¹

(Wong et al., 2015)

NOT Suppressor of

Statement

Reference

RagA-B^Q61L.UAS/Scer\GAL4^pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Scer\GAL4^unspecified/RagA-B^Q61L.UAS is a non-suppressor of decreased cell size | somatic clone phenotype of Rheb^26.2

(Kim et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

RagA-B^Q61L.UAS, Scer\GAL4^nub-AC-62 has wing phenotype, suppressible by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of neuromuscular junction | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of NMJ bouton | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^nSyb.PS/RagA-B^Q61L.UAS is a suppressor of neuromuscular junction | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^nSyb.PS/RagA-B^Q61L.UAS is a suppressor of NMJ bouton | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Cln3^ΔMB1

(Wong et al., 2015)

Scer\GAL4^elav-C155/RagA-B^Q61L.UAS is a suppressor of NMJ bouton | third instar larval stage phenotype of Cln3^ΔMB1

(Wong et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Ectopic expression of RagA-B^{Q61L.Scer\UAS} under the control of the Scer\GAL4^elav-C155 driver fully restores the decreased number of neuromuscular junction boutons characteristic for the Trpml¹ mutant third instar larvae.

Ectopic expression of RagA-B^{Q61L.Scer\UAS} under the control of the Scer\GAL4^elav-C155 driver in the Trpml¹ mutant background significantly increases the proportion of adults that eclose from pupal cases compared to the Trpml¹ mutants.

(Wong et al., 2015)

Expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^Cg.PA suppresses the increase in Lysotracker-positive vesicle volume seen in trpml¹ mutant fat body cells.

(Wong et al., 2012)

Co-expression of hppy^Scer\UAS.cMa cancels the increase in wing size and wing cell number resulting from expression of RagA[Q61L.Scer\UAS] using Scer\GAL4^nub-AC-62.

(Resnik-Docampo and de Celis, 2011)

The overgrowth of the posterior compartment of the wing which is caused by expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^en.PU is still seen in a hppy^SH1261 background.

(Bryk et al., 2010)

The decrease in relative cell area compared to controls that is seen in Rheb^26.2 clones in the fat body is not rescued by expression of RagA^{Q61L.Scer\UAS} under the control of Scer\GAL4^unspecified.

(Kim et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

RagA-B^{Q61L.Scer\UAS}

RagA-B^Q61L.UAS

RagA^{Q61L.Scer\UAS}

dRagA^Q61L

(Kim et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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