FB2025_01 , released February 20, 2025
Allele: Dmel\TrpmlUAS.cVa
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General Information
Symbol
Dmel\TrpmlUAS.cVa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Venkatachalam
FlyBase ID
FBal0221785
Feature type
allele
Associated gene
Dmel\Trpml
Associated Insertion(s)
Carried in Construct
P{UAS-Trpml.V}
Also Known As
UAS-trpml
Key Links
Transgenic product class
cDNA
(Venkatachalam et al., 2008)
wild_type
(Venkatachalam et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Venkatachalam et al., 2008)
wild_type
(Venkatachalam et al., 2008)
Mutagen
in vitro construct
(Venkatachalam et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Trpml.V}
Cytology
Description

UASt regulatory sequences drive expression of a Trpml cDNA.

(Venkatachalam et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Trpml
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The late pupal lethality observed in animals expressing AMPKαJF01951 under the control of Scer\GAL4tub.PU cannot be rescued by co-expression of TrpmlScer\UAS.cVa.

      The pupal-stage lethality of either hemizygous AMPKα1 or AMPKα3 males cannot be rescued by expression of TrpmlScer\UAS.cVa under the control of Scer\GAL4tub.PU.

      (Onyenwoke et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      TrpmlUAS.cVa/Scer\GAL4C164 rescues Trpml1

      (Wong et al., 2015)

      TrpmlUAS.cVa/Scer\GAL4Act5C.PI rescues Trpml1

      (Venkatachalam et al., 2008)
      Partially rescues

      TrpmlUAS.cVa/Scer\GAL4repo partially rescues Trpml1

      (Venkatachalam et al., 2008)

      TrpmlUAS.cVa/Scer\GAL4Cg.PA partially rescues Trpml1

      (Venkatachalam et al., 2008)

      TrpmlUAS.cVa/Scer\GAL4c754 partially rescues Trpml1

      (Venkatachalam et al., 2008)

      TrpmlUAS.cVa/Scer\GAL4Hml.PG partially rescues Trpml1

      (Venkatachalam et al., 2008)
      Fails to rescue

      TrpmlUAS.cVa/Scer\GAL4Mef2.PR fails to rescue Trpml1

      (Wong et al., 2015)

      TrpmlUAS.cVa/Scer\GAL4e22c fails to rescue Trpml1

      (Venkatachalam et al., 2008)
      Comments

      Ectopic expression of TrpmlScer\UAS.cVa using either the Scer\GAL4elav-C155 or the Scer\GAL4C164 driver (but not the Scer\GAL4Mef2.PR driver) rescues the loss of neuromuscular junction boutons characteristic for Trpml1 mutant third instar larvae.

      (Wong et al., 2015)

      Expression of trpmlScer\UAS.cVa under the control of Scer\GAL4repo partially rescues the lethality of trpml1 animals. The climbing ability of trpml1 animals is significantly rescued, but there is still some age-dependent decline in climbing ability in the rescued animals. The defects in excitatory junctional potential amplitude

      of trpml1 larvae are also rescued. The accumulation of late apoptotic/necrotic cells that is seen in 21 day old trpml1 adult brains is rescued, but the accumulation of early apoptotic cells in these brains is not rescued.

      Expression of trpmlScer\UAS.cVa under the control of Scer\GAL4Cg.PA rescues the lethality of trpml1 animals. The climbing ability of trpml1 animals is significantly rescued, but there is still some age-dependent decline in climbing ability in the rescued animals. The accumulation of late apoptotic/necrotic cells that is seen in 21 day old trpml1 adult brains is rescued, but the accumulation of early apoptotic cells in these brains is not rescued.

      Expression of trpmlScer\UAS.cVa under the control of Scer\GAL4c754 rescues the lethality of trpml1 animals. The defects in excitatory junctional potential amplitude

      of trpml1 larvae are also rescued.

      Expression of trpmlScer\UAS.cVa under the control of Scer\GAL4Hml.PG partially rescues the lethality of trpml1 animals.

      (Venkatachalam et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      TrpmlScer\UAS.cVa
      TrpmlUAS.cVa
      trpmlScer\UAS.cVa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Venkatachalam
      Secondary FlyBase IDs
        References (6)