Allele Dmel\Gr93a³

General Information
Symbol	Dmel\Gr93a³	Species	D. melanogaster
Name		FlyBase ID	FBal0221788
Feature type	allele	Associated gene	Dmel\Gr93a

Allele class
Mutagen	recombination, ends-out gene targeting
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-out gene targeting (Lee et al., 2009) recombination (Lee et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Ends-out homologous recombination at the Gr93a locus has resulted in a 539bp deletion that removes the N-terminal 180 residues and first 3 transmembrane segments of Gr93a. The Scer\GAL4 coding region, followed by a w[+] gene has been inserted at a site corresponding to the initiation codon in Gr93a. (Lee et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	chemosensitive behavior defective (Lee et al., 2009)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant flies show normal L-canavanine avoidance in a two-way choice test. (Lee et al., 2012) Gr93a[3] flies show a similar preference for sugars (maltose, trehalose, fructose, glucose or sucrose) as wild-type controls in a binary food-choice assay between 1% agarose plus 10mM sugar and 1% agarose only. Gr93a[3] flies avoid quinine, strychnine, berberine, denatonium, lobeline and papaverine to the same extent as wild-type in a binary food-choice assay between 1mM sucrose alone and 5mM sucrose plus aversive compound. Action potentials induced in the gustatory receptor neurons in response to these aversive compounds are similar in Gr93a[3] and wild-type animals. Gr93a[3] flies show reduced avoidance to caffeine in a binary food-choice assay. Caffeine-induced action potentials are virtually eliminated in the gustatory receptor neurons in Gr93a[3] animals. Mutant flies show reduced avoidance to theophylline in a binary food-choice assay, although avoidance responses to theobromine and paraxanthine are normal. Theophylline-induced action potentials are virtually eliminated in the gustatory receptor neurons in the mutant animals, although theobromine- or paraxanthine-induced action potentials are normal. (Lee et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	Gr93a³ is partially rescued by Scer\GAL4^Gr66a.PD/Gr93a^{Scer\UAS.T:Hsap\MYC} (Lee et al., 2009)
Comments
Stocks ( 3 )
Bloomington	27592 w¹¹¹⁸; Gr93a³ 28801 w^; P{Gr66a-GAL4.D}2; Gr93a³ 28803 w^; P{UAS-Gr93a.Myc}2; Gr93a³
Notes on Origin
Discoverer

Comments
	The Scer\GAL4 coding region inserted in Gr93a[3] is not expressed. (Lee et al., 2009)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Gr93a³ (Lee et al., 2012, Lee et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Lee et al., 2012, J. Neurosci. 32(4): 1429--1435 Gustatory receptors required for avoiding the insecticide L-canavanine. [FBrf0217317] Lee et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(11): 4495--4500 Multiple gustatory receptors required for the caffeine response in Drosophila. [FBrf0207448]

Allele Dmel\Gr93a3

Allele Dmel\Gr93a³