|Feature type||allele||Associated gene||Dmel\Gr93a|
|Mutagen||recombination, ends-out gene targeting|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Ends-out homologous recombination at the Gr93a locus has resulted in a 539bp deletion that removes the N-terminal 180 residues and first 3 transmembrane segments of Gr93a. The Scer\GAL4 coding region, followed by a w[+] gene has been inserted at a site corresponding to the initiation codon in Gr93a.
|Phenotype Manifest In|
Mutant flies show normal L-canavanine avoidance in a two-way choice test.
Gr93a flies show a similar preference for sugars (maltose, trehalose, fructose, glucose or sucrose) as wild-type controls in a binary food-choice assay between 1% agarose plus 10mM sugar and 1% agarose only. Gr93a flies avoid quinine, strychnine, berberine, denatonium, lobeline and papaverine to the same extent as wild-type in a binary food-choice assay between 1mM sucrose alone and 5mM sucrose plus aversive compound. Action potentials induced in the gustatory receptor neurons in response to these aversive compounds are similar in Gr93a and wild-type animals. Gr93a flies show reduced avoidance to caffeine in a binary food-choice assay. Caffeine-induced action potentials are virtually eliminated in the gustatory receptor neurons in Gr93a animals. Mutant flies show reduced avoidance to theophylline in a binary food-choice assay, although avoidance responses to theobromine and paraxanthine are normal. Theophylline-induced action potentials are virtually eliminated in the gustatory receptor neurons in the mutant animals, although theobromine- or paraxanthine-induced action potentials are normal.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Partially rescued by|
|Stocks ( 3 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 2 )|