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General Information
Symbol
Dmel\vtd271TEV.tub.Tag:CS(TEVp),Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0230182
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
rad21TEV
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
A tubulin promoter drives expression of vtd, which is tagged 12bp upstream of the translational stop codon with 10 copies of Tag:MYC. In addition, an SpeI site has been introduced into the vtd coding region after amino acid residue 271, and 3 tandem copies of the Tag:CS(TEVp) protein cleavage tag have been introduced into this site (this sequence allows cleavage of the vtd protein by TEV\NIa). The portion of the construct containing the vtd coding region and a 3'UTR sequence is flanked by an FRT cassette.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Females in which vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC is the sole source of vtd protein (carrying vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC in a vtdunspecified background) show premature synaptonemal complex disassembly in egg chambers after cleavage of the vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC protein by expression of Zzzz\NIaS219V.Scer\UAS.P\T.T:Uuuu\nls6,T:SV5\V5 under the control of Scer\GAL4nos.PG.
Embryos expressing vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYCdisplay centromere disjoining, resulting in sister chromatids moving towards opposite poles.
Embryos in which vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC is the sole source of vtd protein (carrying vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC in a vtdex3 background) show defects in embryonic mitotic cycles after cleavage of the vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC protein by expression of Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16; cells initially undergo mitotic arrest during cycle 14, and chromosomes show unstable kinetochore attachment. After the initial mitotic arrest, chromosome decondensation starts abruptly and unattached chromatids in the central region are cut by the cleavage furrow.
Embryos in which vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC is the sole source of vtd protein (carrying vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC in a vtdex3 background) show defects in embryonic mitotic cycles after cleavage of the vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC protein by expression of Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16; no normal metaphase, anaphase or telophase figures are seen at mitosis 14. Bipolar spindles form, but during prometaphase sister chromatids disjoin prematurely and often segregate to opposite poles. Condensed chromosomes fail to align on a metaphase plate and are found scattered throughout the cells.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
The presence of a TEV protease cleavable form of vtd (by expressing vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC and Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2 in a vtdex3 mutant background) suppresses the chromosome segregation defects seen in embryos expressing aldScer\UAS.cAa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 in a mad2G6595 mutant background. This suppression does not occur in the absence of Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC
vtd271TEV.tub.Tag:CS(TEVp),Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (5)