FB2025_01 , released February 20, 2025
Allele: Dmel\AGO2Tag:FLAG,Tag:HA
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General Information
Symbol
Dmel\AGO2Tag:FLAG,Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0230230
Feature type
allele
Associated gene
Dmel\AGO2
Associated Insertion(s)
Carried in Construct
P{FLAG.HA.AGO2}
Also Known As
Flag-HA-AGO2, FLAG-AGO2
Key Links
Transgenic product class
genomic_DNA
(Czech et al., 2008)
wild_type
(Czech et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Czech et al., 2008)
wild_type
(Czech et al., 2008)
Mutagen
in vitro construct
(Czech et al., 2008)
Progenitor genotype
Carried in construct
P{FLAG.HA.AGO2}
Cytology
Description

Contains the AGO2 coding sequence in the context of the genomic AGO2 locus including flanking regulatory regions. A Tag:FLAG tab and a Tag:HA tag has been inserted at the N-terminal end of the coding sequence.

(Czech et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
AGO2
Encoded product / tool
AGO2
Tagged with
Tag:FLAG
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      r2d21 mutant flies homozygous for AGO2T:Zzzz\FLAG,T:Ivir\HA1 are fertile.

      (Okamura et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      AGO2Tag:FLAG,Tag:HA rescues Df(3L)BSC558/AGO2454

      (Wen et al., 2015)

      AGO2Tag:FLAG,Tag:HA rescues Df(3L)BSC558/AGO2321

      (Wen et al., 2015)
      Comments

      The severely reduced fertility and sperm deficiency of either AGO2454/Df(3L)BSC558 or AGO2321/Df(3L)BSC558 mutant males is rescued by combination with AGO2T:Zzzz\FLAG,T:Ivir\HA1.

      (Wen et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AGO2T:Zzzz\FLAG,T:Ivir\HA1
      AGO2Tag:FLAG,Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (8)