FB2025_05 , released December 11, 2025
Allele: Dmel\Opa1UAS.Tag:FLAG
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General Information
Symbol
Dmel\Opa1UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0230287
Feature type
allele
Associated gene
Dmel\Opa1
Associated Insertion(s)
Carried in Construct
P{UAS-opa1.Flag}
Also Known As
UAS-opa1, UAS-Opa1-FLAG
Key Links
Transgenic product class
wild_type
(Park et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Park et al., 2009)
Mutagen
in vitro construct
(Park et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-opa1.Flag}
Cytology
Description

UAS regulatory sequences drive expression of Opa1 which is tagged at the C-terminal end with Tag:FLAG.

(Park et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Opa1
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  neurodegenerative disease
      modeled by trpP365
      (Huang et al., 2016)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression via Scer\GAL4Cg.PA results in tubular and clumped mitochondria in hemocytes.

      (Banerjee and Chinthapalli, 2014)

      Expression of Opa1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PF leads to protection from rotenone toxicity, with mutant flies exhibiting no significant loss of PPL1 dopaminergic neurons in adult brains after rotenone feeding, in contrast to controls.

      Dopaminergic neurons in flies expressing Opa1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PF exhibit mitochondrial aggregation and fusion, as compared to controls.

      Flies expressing Opa1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU do not exhibit any discernible eye phenotype, as compared to controls.

      (Hwang et al., 2014)

      Adults expressing opa1-likeScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mef2.PR show defects in mitochondrial morphology in the indirect flight muscles, with larger than normal mitochondria being seen between the muscle fibers.

      (Park et al., 2010)

      Expression of opa1-likeScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mef2.PR results in bigger round-shaped mitochondria in the adult thorax than those of controls.

      Expression of opa1-likeScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PF results in rounder and bigger mitochondria than normal in the dopaminergic neurons.

      (Park et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Opa1UAS.Tag:FLAG/Scer\GAL4hs.PB is a non-suppressor of visible phenotype of Pink1B9

      (Park et al., 2009)

      Opa1UAS.Tag:FLAG/Scer\GAL4hs.PB is a non-suppressor of flightless phenotype of Pink1B9

      (Park et al., 2009)
      Other
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Opa1UAS.Tag:FLAG/Scer\GAL4hs.PB is a non-suppressor of wing phenotype of Pink1B9

      (Park et al., 2009)

      Opa1UAS.Tag:FLAG/Scer\GAL4hs.PB is a non-suppressor of adult thorax phenotype of Pink1B9

      (Park et al., 2009)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Opa1Scer\UAS.T:Zzzz\FLAG (positive regulator of mitofusion) driven by Scer\GAL4GMR.PF enhances retinal degeneration (loss of rhabdomeres from photoreceptors) in 8 day old trpP365/+ flies.

      (Huang et al., 2016)

      Co-expression of opa1-likeScer\UAS.T:Zzzz\FLAG significantly rescues the defects in mitochondrial morphology in the indirect flight muscles which is seen in adults expressing porinScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mef2.PR.

      (Park et al., 2010)

      Co-expression of parkScer\UAS.T:Hsap\MYC in adults expressing opa1-likeScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mef2.PR results in smaller than normal mitochondria in the adult thorax.

      The downturned wing, crushed thorax and flightless phenotypes of Pink1B9 mutant flies are not suppressed by expression of opa1-likeScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4hs.PB.

      (Park et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsap\UCP2Scer\UAS.cFa and Opa1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU results in a rough eye phenotype.

      (Hwang et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Opa1Scer\UAS.T:Zzzz\FLAG
      Opa1UAS.Tag:FLAG
      opa1-likeScer\UAS.T:Zzzz\FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (9)