FB2025_01 , released February 20, 2025
Allele: Dmel\Sec61αNIG.9539R
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General Information
Symbol
Dmel\Sec61αNIG.9539R
Species
D. melanogaster
Name
FlyBase ID
FBal0230408
Feature type
allele
Associated gene
Dmel\Sec61α
Associated Insertion(s)
Carried in Construct
P{NIG.9539R}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Arsham and Neufeld, 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Arsham and Neufeld, 2009)
Mutagen
in vitro construct
(Arsham and Neufeld, 2009)
Progenitor genotype
Carried in construct
P{NIG.9539R}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat corresponding to Sec61α.

(Arsham and Neufeld, 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-9539R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Scer\GAL4Act5C.PP

      (Arsham and Neufeld, 2009)

      lysosome & larval fat body | somatic clone | cell autonomous, with Scer\GAL4Act5C.PP

      (Arsham and Neufeld, 2009)
      Detailed Description
      Statement
      Reference

      Sec61αdsRNA.Scer\UAS.NIG clones in fat bodies from well-fed early third instar larvae (generated using Scer\GAL4Act5C.PP) accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes.

      (Arsham and Neufeld, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Expression of Sec61αdsRNA.Scer\UAS.NIG produces dsRNA which results in dsRNA interference (RNAi) of the Sec61α gene. Sec61αdsRNA.Scer\UAS.NIG was generated by the National Institute of Genetics of Japan in Shizuoka.

      (Arsham and Neufeld, 2009)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Sec61αNIG.9539R
      Sec61αdsRNA.Scer\UAS.NIG
      Name Synonyms
      Secondary FlyBase IDs
        References (3)