FlyBase Allele Report: Dmel\sktl[2.3]

General Information

Symbol

Dmel\sktl^2.3

Species

D. melanogaster

Name

FlyBase ID

FBal0230436

Feature type

allele

Associated gene

Dmel\sktl

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Gervais et al., 2008)

Mutagen

ethyl methanesulfonate

(Gervais et al., 2008)

Progenitor genotype

Cytology

Description

276bp deletion in the intron.

(Gervais et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | germline clone

(Fabian et al., 2010)

female sterile (with sktl^Δ5)

(Gervais et al., 2008)

female sterile (with sktl^Δ15)

(Gervais et al., 2008)

lethal (with sktl^Δ20)

(Gervais et al., 2008)

Phenotype Manifest In

actin filament & oocyte | germ-line clone

(Gervais et al., 2008)

dorsal appendage (with sktl^Δ5), with Scer\GAL4^{VP16.mat.αTub67C}

(Claret et al., 2014)

dorsal appendage | germline clone | maternal effect

(Gervais et al., 2008)

egg (with sktl^Δ5)

(Compagnon et al., 2009)

egg | maternal effect (with sktl^Δ15)

(Gervais et al., 2008)

follicle cell (with sktl^Δ5), with Scer\GAL4^{VP16.mat.αTub67C}

(Claret et al., 2014)

oocyte nucleus | germline clone | oogenesis stage S9

(Gervais et al., 2008)

oocyte nucleus | germline clone | oogenesis stage S10

(Gervais et al., 2008)

oocyte nucleus | oogenesis stage S9 (with sktl^Δ15)

(Gervais et al., 2008)

oocyte nucleus | oogenesis stage S10 (with sktl^Δ15)

(Gervais et al., 2008)

spermatid cyst | germline clone

(Fabian et al., 2010)

yolk granule | germline clone

(Compagnon et al., 2009)

Detailed Description

Statement

Reference

The follicle epithelium is multilayered in sktl^2.3/sktl^Δ5 egg chambers in which the germline is rescued by expression of sktl^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}. Dorsal appendages of the resulting egg shells are fused.

(Claret et al., 2014)

Spermatid cysts homozygous for sktl^2.3 exhibit striking defects in polarity and mild cytokinesis defects.

(Fabian et al., 2010)

60% of eggs laid by sktl^Δ5/sktl^2.3 females are flaccid.

Eggs from sktl^2.3 germ-line clones display a reduced number of yolk granules. Only a small number of condensed yolk granules are observed in the oocyte cytoplasm.

(Compagnon et al., 2009)

Eggs deposited by mutant females are ventralised; 51% have loss of dorsal appendages, 43% have fused dorsal appendages and 6% are wild type. The posterior aeropyle is normal in these eggs.

sktl^2.3/sktl^Δ15 females show defects in the localisation of the oocyte nucleus (31% of stage 9-10 oocytes show this phenotype) and they lay eggs that are ventralised.

No defects in the localisation of the oocyte nucleus are seen in homozygous germline clones at stage 8 of oogenesis, however 80% of stage 9 and 96% of stage 10 oocytes show mislocalisation of the nucleus, with the nucleus being tightly associated with the lateral cortex.

Up to stage 8, the microtubule network in the oocyte in homozygous germline clones is organised as in controls. However, from stage 9 onwards, the anterior-to-posterior microtubule organisation appears to be lost in homozygous germline clones and the microtubule array is instead organised around the mislocalised nucleus.

The organisation of actin filaments appears to be normal during early oogenesis in homozygous female germline clones. However, from stage 8 onwards, two distinct types of defects are seen. In 36% of the oocytes, the cortical actin network is disrupted at the border between the anterior margin and the lateral cortex. In 15% of oocytes the actin microfilaments in the oocyte are loosely bound to the lateral cortex, including to the posterior pole and they delaminate into the cytoplasm. The continuity of the plasma membrane of the oocyte is unaffected.

(Gervais et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

sktl^Δ15/sktl^2.3 has oocyte nucleus | oogenesis stage S9 phenotype, enhanceable by baz⁴/baz[+]

(Gervais et al., 2008)

sktl^Δ15/sktl^2.3 has oocyte nucleus | oogenesis stage S10 phenotype, enhanceable by baz⁴/baz[+]

(Gervais et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

baz⁴/+ enhances the nucleus mislocalisation defect seen in stage 9-10 sktl^2.3/sktl^Δ15 oocytes, such that 63% have a mislocalised nucleus.

(Gervais et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sktl^2.3/sktl^Δ5 is rescued by Scer\GAL4^{VP16.nanos.UTR}/sktl^UASp.cGa

(Gervais et al., 2008)

sktl^2.3 is rescued by Scer\GAL4^{VP16.nanos.UTR}/sktl^UASp.cGa

(Gervais et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

l(2)2.3

(Gervais et al., 2008)

sktl2.3

(Claret et al., 2014)

sktl^2.3

(Jouette et al., 2019, Gupta et al., 2018, Fabian et al., 2010, Compagnon et al., 2009, Gervais et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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