Imprecise excision of the progenitor insertion. An approximately 350bp fragment of the original insertion remains (which is approximately 50bp upstream of Ppat-Dpck).
sterile (with Df(3L)ZN47)
Homozygous females deposit 0.36 +/- 0.04 eggs/24 hours, of which 20.1% are able to hatch.
17% of eggs from 5 day old homozygous females are elongated along the anteroposterior axis and have a collapsed phenotype.
Cytoplasmic dumping and centripetal migration of follicle cells are often severely disrupted in homozygous egg chambers. Ring canals are often plugged with nurse cell nuclei.
The level of neutral lipids is not severely affected in homozygous egg chambers, but abnormal large lipid droplets are seen.
Ectopic vein formation is seen initiating from the posterior cross vein in homozygous wings.
Homozygous larvae show slightly decreased locomotor activity (crawling) compared to controls.
Homozygous adults become paralysed after heat exposure (2 hours at 37[o]C) and recover to near normal activity 15 minutes after being placed at 22[o]C.
Homozygous females have impaired fertility.
Ppat-Dpck43/Df(3L)ZN47 animals show fertility defects.
7 day old homozygous flies show a decrease in the fraction of flies able to initiate flight compared to controls. 14 day old homozygous flies show no change in the ability of flies to initiate flight compared to the 7 day old homozygous flies, but flight performance is reduced in the aged flies compared to the young flies.
7 day old homozygous flies show a reduced ability to climb compared to control flies in a negative geotaxis assay. This phenotype is increased in severity in 14 day old homozygous flies.
Homozygous flies have a reduced median lifespan (32 days) compared to control flies (50 days).
Homozygous flies often have held-out or (less frequently) held-up wings.
The level of triglycerides is reduced approximately 25% in 14 day old homozygous adults compared to controls.
Homozygous adults show increased sensitivity to 5% H[[2]]O[[2]], 100mM dithiothreitol and 20mM paraquat compared to control adults.
Homozygous larvae show normal levels of survival on medium containing 100mM cysteine.
Homozygous third larval instar brains show a high incidence of abnormal mitotic chromosomes compared to controls.
Treatment with 20 Gy irradiation results in an increased incidence of abnormal mitotic chromosomes in homozygous third larval instar brains compared to controls.
Homozygous larval brains show an increased level of apoptosis compared to controls.
The survival rate of adults after exposure of larvae to 20Gy irradiation is reduced in homozygotes compared to wild-type flies.
Ppat-Dpck43 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Sod1+t2.4/CattOa/Trxr1+t10
Simultaneous co-expression of Sod+t2.4, CattOa and Trxr-1+t10 does not suppress the impaired ability of Ppat-Dpck43 homozygous adults to climb in a negative geotaxis assay.
