Allele Dmel\mars^{Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\mars^{Scer\UAS.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0230464
Feature type	allele	Associated gene	Dmel\mars

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Yang et al., 2005) in vitro construct - regulatory fusion (Yang et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS sequences drive expression of full length mars which is tagged at the N-terminal end with T:Zzzz\FLAG. (Yang et al., 2005)
Carried in construct	P{UAS-mars.Flag} (Yang et al., 2005, Yang and Fan, 2008)
Tagged with	T:Zzzz\FLAG (Yang et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death, with Scer\GAL4^GMR.PF (Yang et al., 2005) lethal, with Scer\GAL4^ap-md544 (Yang et al., 2005) lethal, with Scer\GAL4^dpp.blk1 (Yang et al., 2005) lethal, with Scer\GAL4^ey.PH (Yang et al., 2005) lethal, with Scer\GAL4^ptc-559.1 (Yang et al., 2005) mitotic cell cycle defective, with Scer\GAL4^GMR.PF (Yang et al., 2005) visible, with Scer\GAL4^GMR.PF (Yang et al., 2005)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Yang et al., 2005) eye disc, with Scer\GAL4^GMR.PF (Yang et al., 2005)
Detailed Description
	Statement Reference Flies expressing mars[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] have small, rough eyes. This phenotype is partially suppressed by coexpression of mars[Scer\UAS.WIZ]. The number of mitotic cells at the second mitotic wave in eye discs expressing mars[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] is increased compared to controls. The distribution of BrdU labeling is similar in the mutant and control eye discs. 68.8 +/- 9.5% of mitotic figures at the second mitotic wave are abnormal in eye discs expressing mars[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF]. Circular or fragmented chromosomes, misalignment of chromosomes, aberrant anaphase with lagging chromosomes and incorrect separation of centriole pairs is seen. The fraction of mitotic cells in anaphase or telophase is reduced to 2.7 +/- 1.8% in eye discs expressing mars[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] (compared to 21.0 +/- 11.0% of mitotic cells being in anaphase or telophase in wild-type discs). Eye discs expressing mars[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] show a significant increase in the number of apoptotic cells posterior to the morphogenetic furrow compared to wild-type eye discs. (Yang et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, mars^{Scer\UAS.T:Zzzz\FLAG} has increased cell death phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference The increased cell death posterior to the morphogenetic furrow that is seen in eye discs expressing mars[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] is suppressed by co-expression of BacA\p35[Scer\UAS.cHa]. (Yang et al., 2005)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mars^{Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Yang and Fan, 2008, Exp. Cell Res. 314(17): 3209--3220 Drosophila mars is required for organizing kinetochore microtubules during mitosis. [FBrf0207109] Yang et al., 2005, Exp. Cell Res. 307(1): 183--193 Using Drosophila eye as a model system to characterize the function of mars gene in cell-cycle regulation. [FBrf0187553]

Allele Dmel\marsScer\UAS.T:Zzzz\FLAG

Allele Dmel\mars^{Scer\UAS.T:Zzzz\FLAG}