FlyBase Allele Report: Scer\GAL4[smid-C161]

Drives expression in sensory neurons. Also drives expression in small field neurons associated with the ellipsoid body, protocerebral bridge, and nodulus.

(FlyBase Curators, 2017)

ScerGAL4^smid-C161 drives expression in larval multidendritic neurons ldaB, ddaC and ddaE.

(Nagel et al., 2012)

Third instar expression is observed in vbd- and dbd-neurons, one lateral chordotonal organ, multidendritic neurons, and most likely to the vpda-neurons. See diagram in Fig 3C of FBrf0160715.

(Landgraf et al., 2003)

Adult expression is observed in small field neurons that connect the ellipsoid body with the nodulus and the protocerebral bridge.

(Renn et al., 1999)

Reporter gene expression was examined in early to mid larval stages. Expression is observed in sensory neurons and neuroblasts in the cephalic and thoracic neuromeres of the CNS and in imaginal discs. No expression was observed in embryos.

(Long et al., 1998)

Expression of ScerGAL4^smid-C161 is found in internal sensory neurons associates with the trochanter/femur articulation, an internal receptor associated with the tibial/tarsal joint and internal receptors associated with each of the tarsal segment. Expression of ScerGAL4^smid-C161 is also seen in neurons at the articulations of the more distal tibial and tarsal segments of the leg, which are thought to be stretch receptors. Expression of ScerGAL4^smid-C161 in the hair plates of the trochanter and the coxa is restricted to a small group of cell bodies at the base of the external sensilla that extend neurites into the sockets of the sensilla. Expression of ScerGAL4^smid-C161 is seen in approximately five sensilla of a cluster of eleven campaniform sensilla (believed to be the FeCS11) located on the ventral surface of the proximal femur. On the haltere, expression of ScerGAL4^smid-C161 is detected in two large groups of cells associated with a cluster of sensilla on the scabellum and pediculus. Furthermore, expression is detected in two clusters of scolopale cells in the scabellum. Abdominal expression of ScerGAL4^smid-C161 is seen, in each segment, in bilaterally symmetrical sensory structures: a pair of small neurons on either side of the midline that extend along the lateral muscles; a single neuron attached to one of the dorsal muscles; a midbody positioned neuron that extends processes both ventrally to the CNS and dorsally to arborise on the dorsal alar muscle. CNS expression is seen in the nerves originating from the leg and the haltere, nerves from the abdominal segments, and axons from the prothoracic stretch receptor which enter the CNS within the leg nerve.

(Smith and Shepherd, 1996)

Marker for

adult protocerebral bridge 1 glomerulus-ellipsoid body tile-nodulus 1 neuron

(Renn et al., 1999)

dendritic arborizing neuron

(Nagel et al., 2012)

ellipsoid body

(Renn et al., 1999)

small field neuron

(Renn et al., 1999)

Reflects expression of

smid

(Nagel et al., 2012, Tsubouchi et al., 2012, Shimono et al., 2009, Hwang et al., 2007, Williams et al., 2000, Williams and Shepherd, 1999, Long et al., 1998)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | third instar larval stage | chemical conditional, with Crei\ChR2^UAS.cHa.EYFP

(Hwang et al., 2007)

abnormal cell death | pupal stage, with east^UAS.EGFP

(Wasser and Chia, 2007)

abnormal neuroanatomy, with Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}

(Williams et al., 2000)

abnormal neuroanatomy, with GSd236^GSd236

(Ou et al., 2008)

abnormal neuroanatomy, with kn^UAS.cMa

(Jinushi-Nakao et al., 2007)

abnormal neuroanatomy, with Pi3K92E^UAS.cUa

(Brierley et al., 2009)

abnormal neuroanatomy | pupal stage, with mop^HMS00706

(Loncle et al., 2015)

abnormal neuroanatomy | pupal stage, with shrb^RNAi.UAS

(Loncle et al., 2015)

abnormal neuroanatomy | third instar larval stage, with Opa1^{K273A.UAS.Tag:HA}

(Tsuyama et al., 2017)

abnormal neuroanatomy | third instar larval stage, with prel^UAS.Tag:HA

(Tsuyama et al., 2017)

abnormal neuroanatomy | third instar larval stage, with ttm50^UAS.Tag:HA

(Tsuyama et al., 2017)

abnormal pain response | third instar larval stage, with Ctet\tetX^TNT-LC.UAS

(Hwang et al., 2007)

abnormal touch response | larval stage, with Ctet\tetX^TNT-LC.UAS

(Tsubouchi et al., 2012)

viable, with COX5B^GD6659

(Kemppainen et al., 2014)

viable, with COX6B^GD13061

(Kemppainen et al., 2014)

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaA | third instar larval stage, with Opa1^{K273A.UAS.Tag:HA}

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaA | third instar larval stage, with prel^UAS.Tag:HA

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaA | third instar larval stage, with ttm50^UAS.Tag:HA

(Tsuyama et al., 2017)

cell body, with Pi3K92E^UAS.cUa

(Brierley et al., 2009)

dendrite, with kn^UAS.cMa

(Jinushi-Nakao et al., 2007)

dendrite, with Pi3K92E^UAS.cUa

(Brierley et al., 2009)

dendrite | pupal stage, with mop^HMS00706

(Loncle et al., 2015)

dendrite | pupal stage, with shrb^RNAi.UAS

(Loncle et al., 2015)

dendrite | third instar larval stage, with Opa1^{K273A.UAS.Tag:HA}

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with prel^UAS.Tag:HA

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with ttm50^UAS.Tag:HA

(Tsuyama et al., 2017)

larval dorsal multidendritic neuron ddaA, with GSd236^GSd236

(Ou et al., 2008)

larval dorsal multidendritic neuron ddaC, with Pi3K92E^UAS.cUa

(Brierley et al., 2009)

larval multidendritic class III neuron, with kn^UAS.cMa

(Jinushi-Nakao et al., 2007)

larval multidendritic class III neuron | larval stage, with kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2007)

larval multidendritic class II neuron, with kn^UAS.cMa

(Jinushi-Nakao et al., 2007)

larval multidendritic class II neuron | larval stage, with kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2007)

larval multidendritic class I neuron | pupal stage, with mop^HMS00706

(Loncle et al., 2015)

larval multidendritic class I neuron | pupal stage, with shrb^RNAi.UAS

(Loncle et al., 2015)

metathoracic neuromere, with Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}

(Williams et al., 2000)

prothoracic femoral chordotonal organ & axon, with Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}

(Williams et al., 2000)

prothoracic neuromere, with Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}

(Williams et al., 2000)

prothoracic neuromere, with Ecol\lacZ^{UAS.O.Tag:MT(btau)}

(Williams et al., 2000)

prothoracic neuromere, with Hsap\MAPT^UAS.cAa

(Williams et al., 2000)

salivary gland | pupal stage, with east^UAS.EGFP

(Wasser and Chia, 2007)

Detailed Description

Statement

Reference

Inhibition of neural activity in Scer\GAL4^smid-C161-expressing neurons (in which expression is suppressed in the ventral nerve cord using Scer\GAL80^tsh-GAL80) does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

External Data

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Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions