FlyBase Allele Report: Dmel\Atg18a[KK100064]

General Information

Symbol

Dmel\Atg18a^KK100064

Species

D. melanogaster

Name

FlyBase ID

FBal0230561

Feature type

allele

Associated gene

Dmel\Atg18a

Associated Insertion(s)

Carried in Construct

P{KK100064}

Also Known As

UAS-atg18^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100064}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100064

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | male, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

short lived, with Scer\GAL4^Act88F.PU

(Xu et al., 2019)

short lived, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

short lived, with Scer\GAL4^tin.CΔ4

(Xu et al., 2019)

Phenotype Manifest In

adult fat body | nutrition conditional, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

adult heart muscle cell | day 40 of adulthood, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

adult heart muscle cell | progressive, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

autophagosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

indirect flight muscle cell | day 40 of adulthood, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

lysosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

mitochondrion | adult stage, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

Detailed Description

Statement

Reference

Flies expressing Atg18a^KK100064 under the control of Scer\GAL4^Mef2.PU display climbing defects in males (but not females), together with progressive heart defects: thicker heart walls and a distorted lumen with a rough surface in older flies, increased systolic dimension (but not diastolic dimension) throughout adult life, and significantly reduced fractional shortening at day 40 (but not in younger flies). Mitochondria in cardiomyocytes are abnormally elongated and narrowed, the mitochondrial network appears fused/enlarged and the mitochondrial volume density is significantly increased in 40-day old flies. Alongside, indirect flight muscles display fragmented mitochondria and reduced mitochondrial volume density.

(Xu et al., 2019)

Adulthood-only expression of Atg18a^KK100064 under the combined control of Scer\GAL4^{Myo31DF-NP0001} and Gal80[ts] does not significantly affect the posterior midgut cell size, as compared to controls. Adulthood-only expression under the combined control of Scer\GAL4^tub.PU and Gal80[ts] leads to the failure to induce autophagy (assessed by the lysosome and autophagosome marker, Lysotracker) in the adult fat body in response to starvation, as compared to controls.

(Wen et al., 2017)

Flies expressing Atg18a^KK100064 under the control of Scer\GAL4^GMR.PU show no defects in ommatidial structure.

(Chen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Atg18a^KK100064, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size | third instar larval stage phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1