FlyBase Allele Report: Dmel\Atx2[KK100423]

General Information

Symbol

Dmel\Atx2^KK100423

Species

D. melanogaster

Name

FlyBase ID

FBal0230738

Feature type

allele

Associated gene

Dmel\Atx2

Associated Insertion(s)

Carried in Construct

P{KK100423}

Also Known As

Atx2 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100423}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100423

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Xu et al., 2019)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^50.UAS.EGFP

(Lee et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior, with Scer\GAL4^P2.4.Pdf

(Zhang et al., 2013)

abnormal circadian behavior, with Scer\GAL4^tim.PE

(Zhang et al., 2013)

abnormal circadian rhythm | adult stage, with Scer\GAL4^tim.UAS

(Rounds et al., 2022)

abnormal locomotor rhythm, with Scer\GAL4^Mz520

(Lim and Allada, 2013)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Lim and Allada, 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Circadian entrained flies expressing Atx2^KK100423 under the control of Scer\GAL4^tim.UAS show decreased rhythmicity upon shift to constant darkness conditions.

(Rounds et al., 2022)

Expression of Atx2^KK100423 under the control of Scer\GAL4^cry.PE does not significantly affect the rhythmicity of adult circadian locomotor activity under free-running (~constant darkness) conditions.

(Lee et al., 2017)

Expression of Atx2^KK100423 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^Mz520 results in a long-period locomotor rhythm in constant dark conditions. Loss of rhythmicity is also seen when Atx2^KK100423 and Dcr-2^Scer\UAS.cDa expressed under the control of Scer\GAL4^P2.4.Pdf.

(Lim and Allada, 2013)

When Atx2^KK100423 is expressed in all circadian neurons under the control of Scer\GAL4^tim.PE (either with or without Dcr-2^Scer\UAS.cDa) the period of circadian locomotor darkness is lengthened to ~27 hours. A similar increase is seen when Atx2^KK100423 is expressed under the control of Scer\GAL4^P2.4.Pdf. No phenotype is observed when Atx2^KK100423 is expressed under the control of Scer\GAL4^tim.PE in combination with Scer\GAL80^Pdf.PS to block transcription in Pdf cells.

(Zhang et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Atx2^KK100423 has abnormal circadian behavior phenotype, non-enhanceable by tyf^Δ11

(Zhang et al., 2013)

Suppressed by

Statement

Reference

Atx2^KK100423, Scer\GAL4^tim.UAS has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Nab2^KK101974, Scer\GAL4^tim.UAS

(Rounds et al., 2022)

Atx2^KK100423, Scer\GAL4^tim.PE has abnormal circadian behavior phenotype, suppressible by per^UAS.cYa, Scer\GAL4^tim.PE

(Zhang et al., 2013)

NOT suppressed by

Statement

Reference

Atx2^KK100423 has abnormal circadian behavior phenotype, non-suppressible by tyf^Δ11

(Zhang et al., 2013)

Enhancer of

Statement

Reference

Atx2^KK100423, Scer\GAL4^cry.PE is an enhancer of abnormal locomotor rhythm | adult stage phenotype of Scer\GAL4^cry.PE, me31B^NIG.4916R

(Lee et al., 2017)

NOT Enhancer of

Statement

Reference

Atx2^KK100423, Scer\GAL4^P2.4.Pdf is a non-enhancer of abnormal circadian rhythm | adult stage phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^P2.4.Pdf

(Xu et al., 2019)

Atx2^KK100423/Scer\GAL4^tim.PE is a non-enhancer of abnormal circadian behavior phenotype of tyf^Δ11

(Zhang et al., 2013)

NOT Suppressor of

Statement

Reference

Atx2^KK100423, Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal circadian rhythm | adult stage phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^P2.4.Pdf

(Xu et al., 2019)

Atx2^KK100423/Scer\GAL4^tim.PE is a non-suppressor of abnormal circadian behavior phenotype of tyf^Δ11

(Zhang et al., 2013)

Other

Statement

Reference

Atx2^KK100423, Hsap\HTT^N548.Q0.UAS, Scer\GAL4^P2.4.Pdf has abnormal circadian rhythm | adult stage phenotype

(Xu et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Circadian entrained flies co-expressing Atx2^KK100423 and Nab2^KK101974 under the control of Scer\GAL4^tim.UAS show increased circadian period and decreased rhythmicity power upon shift to constant darkness conditions.

(Rounds et al., 2022)

The poor rhythmicity of adult circadian locomotor activity under free-running (~constant darkness) conditions observed in flies expressing me31B^NIG.4916R under the control of Scer\GAL4^cry.PE is further worsened by co-expression of Atx2^KK100423.

(Lee et al., 2017)

Knocking down Atx2 expression (using Atx2^KK100423 under the control of Scer\GAL4^tim.PE) in a tyf^Δ11 mutant background results in a similar increase in period length as is seen in either mutant alone.

Expression of per^Scer\UAS.cYa suppresses the lengthening of the circadian locomotor darkness period seen when Atx2^KK100423 is expressed under the control of Scer\GAL4^tim.PE.

(Zhang et al., 2013)

Xenogenetic Interactions

Statement

Reference

The co-expression of Hsap\HTT^Q0.UAS and Atx2^KK100423 under the control of Scer\GAL4^P2.4.Pdf leads to a moderate impairment of circadian behavior, as compared to Hsap\HTT^Q0.UAS single expression and wild-type controls.

(Xu et al., 2019)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v108843

P{KK100423}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Atx2^KK100423

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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