FlyBase Allele Report: Dmel\Akt[KK100495]

General Information

Symbol

Dmel\Akt^KK100495

Species

D. melanogaster

Name

FlyBase ID

FBal0230785

Feature type

allele

Associated gene

Dmel\Akt

Associated Insertion(s)

Carried in Construct

P{KK100495}

Also Known As

UAS-Akt-RNAi, UAS-AKT1-RNAi, UAS-Akt RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100495}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100495

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

ameliorates high grade glioma

modeled by Wdr62^UAS.Tag:HA

(Shohayeb et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | third instar larval stage, with Scer\GAL4^hh.PU

(Romero-Pozuelo et al., 2017)

chemical resistant, with Scer\GAL4^elav-C155

(Eddison et al., 2011)

decreased cell number | third instar larval stage, with Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

decreased cell size | third instar larval stage, with Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

decreased size | third instar larval stage, with Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

Phenotype Manifest In

larval brain | third instar larval stage, with Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

larval glial cell | decreased number | third instar larval stage, with Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

nucleolus | third instar larval stage, with Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^hh.PU

(Romero-Pozuelo et al., 2017)

Detailed Description

Statement

Reference

Expression of Akt^KK100495 under the control of Scer\GAL4^repo.PU leads to a decrease in the brain volume, glial cell size and glial nucleolus size in third instar larvae.

(Shohayeb et al., 2020)

Expression of Akt1^KK100495 (driven by Scer\GAL4^Dll-md23) in type II neuroblast lineage MARCM clones has no significant effect on the clone size or the number of progenitor cells it contains in third instar larvae.

(Rives-Quinto et al., 2017)

Expression of Akt1^KK100495 under the control of Scer\GAL4^hh.PU results in decreased size of the posterior compartment in third instar larval wing discs.

(Romero-Pozuelo et al., 2017)

Expression of Akt1^KK100495 under the control of Scer\GAL4^phm.PO does not significantly change the number of lipid droplets in the prothoracic gland cells of third instar larvae compared to controls.

(Danielsen et al., 2016)

Expression of Akt1^KK100495 in the eye, under the control of Scer\GAL4^GMR.PF, does not result in a visible phenotype.

(Nandi et al., 2014)

Neuronal expression of Akt1^KK100495 under the control of Scer\GAL4^elav-C155 decreases sensitivity to ethanol sedation.

(Eddison et al., 2011)

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Akt^KK100495, Scer\GAL4^repo.PU has decreased size | third instar larval stage phenotype, suppressible | partially by Wdr62^UAS.Tag:HA, Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

Akt^KK100495, Scer\GAL4^repo.PU has decreased cell number | third instar larval stage phenotype, suppressible | partially by Wdr62^UAS.Tag:HA, Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

Akt^KK100495, Scer\GAL4^repo.PU has decreased cell size | third instar larval stage phenotype, suppressible | partially by Wdr62^UAS.Tag:HA, Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

Suppressor of

Statement

Reference

Akt^KK100495, Scer\GAL4^sns.PU is a suppressor of increased cell size | larval stage phenotype of Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU

(Gass et al., 2022)

Scer\GAL4^Dll-md23/Akt^KK100495 is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Scer\GAL4^Dll-md23/Akt^KK100495 is a suppressor | somatic clone of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Scer\GAL4^Dll-md23/Akt^KK100495 is a suppressor | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Scer\GAL4^Dll-md23/Akt^KK100495 is a suppressor | somatic clone of increased cell number | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Akt^KK100495, Scer\GAL4^Hml.Δ is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Akt^KK100495, Scer\GAL4^repo.PU has nucleolus | third instar larval stage phenotype, suppressible | partially by Wdr62^UAS.Tag:HA, Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

Akt^KK100495, Scer\GAL4^repo.PU has larval brain | third instar larval stage phenotype, suppressible | partially by Wdr62^UAS.Tag:HA, Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

Akt^KK100495, Scer\GAL4^repo.PU has larval glial cell | third instar larval stage | decreased number phenotype, suppressible | partially by Wdr62^UAS.Tag:HA, Scer\GAL4^repo.PU

(Shohayeb et al., 2020)

Suppressor of

Statement

Reference

Akt^KK100495, Scer\GAL4^sns.PU is a suppressor | partially of slit diaphragm | larval stage | decreased number phenotype of Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU

(Gass et al., 2022)

Akt^KK100495, Scer\GAL4^sns.PU is a suppressor | partially of embryonic/larval garland cell | larval stage | decreased number phenotype of Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU

(Gass et al., 2022)

Scer\GAL4^Dll-md23/Akt^KK100495 is a suppressor | somatic clone of larval brain | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Akt^KK100495, Scer\GAL4^Ugt36A1.PK is a suppressor of lamellocyte | larval stage phenotype of ND-75^GD6309, Scer\GAL4^Ugt36A1.PK

(Sinenko et al., 2011)

Akt^KK100495, Scer\GAL4^Hml.Δ is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrowth and increased number of progenitor cells characteristic for cno^R2;scrib¹ type II neuroblast lineage MARCM clones is significantly suppressed by Scer\GAL4^Dll-md23-driven expression of Akt1^KK100495 in the mutant clones.

(Rives-Quinto et al., 2017)

The Acn^Scer\UAS.cNa-induced rough eye phenotype is suppressed by co-expression of Akt1^KK100495 (when both are expressed under the control of Scer\GAL4^GMR.PF).

(Nandi et al., 2014)

Expression of Akt1^KK100495 suppresses the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Dot.PK.

(Sinenko et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of Akt1^KK100495 strongly suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103703

P{KK100495}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Akt1^KK100495

Akt^KK100495

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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