FlyBase Allele Report: Dmel\Sin3A[KK100700]

General Information

Symbol

Dmel\Sin3A^KK100700

Species

D. melanogaster

Name

FlyBase ID

FBal0230884

Feature type

allele

Associated gene

Dmel\Sin3A

Associated Insertion(s)

Carried in Construct

P{KK100700}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100700}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100700

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Das et al., 2013)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Ret^MEN2B.GMR

(Das et al., 2013)

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

exacerbates brain cancer

modeled by Ras85D^V12.cUa.UAS, scrib⁶⁷³

(Manzanero-Ortiz et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration, with Scer\GAL4^ptc-559.1

(Das et al., 2013)

visible | adult stage, with Scer\GAL4^GMR.PF

(Das et al., 2013)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Das et al., 2013)

wing disc, with Scer\GAL4^ptc-559.1

(Das et al., 2013)

Detailed Description

Statement

Reference

Expression of Sin3A^KK100700 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^ptc-559.1 results in mild but consistent cell migration from the ptc domain of the wing disc compared to controls. Knockdown using a stronger line (created by mobilising the transgene) leads to more aggressive cell invasion as many cells travel a significant distance before eventually undergoing apoptosis. Cells lose their elongate morphology and shift basally at the "tumor" boundary. Cells have reduced adherens junctions, altered polarity, and extrude basally away from the ptc boundary.

Expression of Sin3A^KK100700 under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype, often with growths emerging from the eye.

(Das et al., 2013)

Expression of Sin3A^KK100700 under the control of Scer\GAL4^Hml.Δ has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, enhanceable by Scer\GAL4^ptc-559.1/shg^UAS.cBa

(Das et al., 2013)

Scer\GAL4^GMR.PF, Sin3A^KK100700 has visible | adult stage phenotype, enhanceable by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, enhanceable by Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, enhanceable by puc^E69/puc[+]

(Das et al., 2013)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, non-enhanceable by pyd^unspecified/pyd[+]

(Das et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, suppressible by Jra^unspecified/Jra[+]

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, suppressible by Scer\GAL4^ptc-559.1/bsk^K53R.UAS

(Das et al., 2013)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, suppressible by bsk^K53R.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, suppressible by shg[+]/shg^R69

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, suppressible by Scer\GAL4^ptc-559.1/pan^ΔN.UAS

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, suppressible by bsk²/bsk[+]

(Das et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, non-suppressible by pyd^unspecified/pyd[+]

(Das et al., 2013)

Enhancer of

Statement

Reference

Sin3A^KK100700, Scer\GAL4^ptc-559.1 is an enhancer of abnormal size phenotype of Ret^MEN2B.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Sin3A^KK100700, Scer\GAL4^ptc-559.1 is an enhancer of abnormal size phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Sin3A^KK100700, Scer\GAL4^ptc-559.1 is an enhancer of abnormal cell migration phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Sin3A^KK100700, Scer\GAL4^ptc-559.1 is an enhancer of abnormal cell migration phenotype of Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Suppressor of

Statement

Reference

Sin3A^KK100700, Scer\GAL4^Hml.Δ is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Other

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700, bsk^K53R.UAS has abnormal size phenotype

(Das et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Sin3A^KK100700 has eye phenotype, enhanceable by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, enhanceable by Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, enhanceable by bsk^K53R.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, enhanceable by puc^E69/puc[+]

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, enhanceable by Scer\GAL4^ptc-559.1/shg^UAS.cBa

(Das et al., 2013)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, non-enhanceable by pyd^unspecified/pyd[+]

(Das et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, suppressible by bsk²/bsk[+]

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, suppressible by Jra^unspecified/Jra[+]

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, suppressible by Scer\GAL4^ptc-559.1/bsk^K53R.UAS

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, suppressible by shg[+]/shg^R69

(Das et al., 2013)

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, suppressible by Scer\GAL4^ptc-559.1/pan^ΔN.UAS

(Das et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, non-suppressible by pyd^unspecified/pyd[+]

(Das et al., 2013)

Enhancer of

Statement

Reference

Sin3A^KK100700, Scer\GAL4^ptc-559.1 is an enhancer of wing disc phenotype of Ret^MEN2B.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Sin3A^KK100700, Scer\GAL4^ptc-559.1 is an enhancer of wing disc phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Sin3A^KK100700, Scer\GAL4^ptc-559.1 is an enhancer of wing disc phenotype of Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Suppressor of

Statement

Reference

Sin3A^KK100700, Scer\GAL4^Hml.Δ is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Other

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700, bsk^K53R.UAS has wing disc phenotype

(Das et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Sin3A^KK100700 enhances the wing disc phenotypes seen when Ret^{MEN2B.Scer\UAS} is expressed under the control of Scer\GAL4^ptc-559.1. Large numbers of cells are shifted basally below the epithelium and migrate significant distances from the ptc domain.

Expression of th^Scer\UAS.cHa enhances the eye phenotype seen when Sin3A^KK100700 is expressed under the control of Scer\GAL4^GMR.PF; approximately a third of all adults examined show enhanced overgrowth and secondary tumors that grow stably at distant sites.

Co-expression of Csk^{dsRNA.Scer\UAS} and Sin3A^KK100700 (with Dcr-2^Scer\UAS.cDa) results in a much stronger wing disc cell migration phenotype than is seen when either transgene is expressed alone. Upon co-expression of bsk^{K53R.Scer\UAS}, massive overgrowth is seen, but the invasion phenotype is suppressed.

The cell invasion phenotype seen when Sin3A^KK100700 is strongly expressed (using a line in which the transgene has been mobilised to the third chromosome) under the control of Scer\GAL4^ptc-559.1 is suppressed in a bsk²/+ background.

Expression of bsk^{K53R.Scer\UAS} suppresses the cell invasion phenotype seen in wing discs when Sin3A^KK100700 is expressed under the control of Scer\GAL4^ptc-559.1.

The cell invasion phenotype seen when Sin3A^KK100700 is expressed under the control of Scer\GAL4^ptc-559.1 is enhanced in a puc^E69/+ background.

Expression of shg^Scer\UAS.cBa enhances the cell invasion phenotype seen when mild Sin3A^KK100700 is expressed under the control of Scer\GAL4^ptc-559.1. The migrating cells from the ptc compartment travel greater distances compared to when Sin3A^KK100700 is expressed alone.

The cell invasion phenotype seen when Sin3A^KK100700 is expressed under the control of Scer\GAL4^ptc-559.1 is unaffected in a pyd^unspecified/+ background.

Expression of pan^ΔN.Scer\UAS fully suppresses the cell invasion phenotype seen in wing discs when Sin3A^KK100700 is expressed under the control of Scer\GAL4^ptc-559.1.

Expression of Sin3A^KK100700 enhances the overproliferation and cell migration phenotypes seen when dm^Scer\UAS.cZa is expressed under the control of Scer\GAL4^ptc-559.1.

(Das et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of Sin3A^KK100700 moderately suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v105852

P{KK100700}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Sin3A^KK100700

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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