FB2025_01 , released February 20, 2025
Allele: Dmel\wtsKK101055
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General Information
Symbol
Dmel\wtsKK101055
Species
D. melanogaster
Name
FlyBase ID
FBal0231043
Feature type
allele
Associated gene
Dmel\wts
Associated Insertion(s)
Carried in Construct
P{KK101055}
Also Known As
UAS-wts-RNAi, UAS-Wts RNAi, UAS-wtsRNAi, wts RNAi, UAS-wts-IR, wts-IR
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK101055}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK101055
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  high grade glioma
      modeled by Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS), btl::Egfrλ.UAS
      (Vigneswaran et al., 2021)
      model of  cancer
      is ameliorated by Xbp1UAS.s.Tag:V5
      (Yang et al., 2022)
      is ameliorated by Hsc70-3UAS.ORF.Tag:HA
      (Yang et al., 2022)
      is ameliorated by Ire1UAS.Tag:V5
      (Yang et al., 2022)
      is ameliorated by Rox8EY02351
      (Guo et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal immune response | adult stage, with Scer\GAL4hs.PB

      (Lee et al., 2018)

      abnormal immune response | adult stage, with Scer\GAL4NP0001

      (Lee et al., 2018)

      abnormal immune response | adult stage, with Scer\GAL4r4

      (Liu et al., 2016)

      abnormal immune response | adult stage, with Scer\GAL4yolk

      (Liu et al., 2016)

      abnormal neuroanatomy | first instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal neuroanatomy | second instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal planar polarity | adult stage, with Scer\GAL4ey.PH

      (Brittle et al., 2012)

      abnormal size | adult stage, with Scer\GAL4GMR.PF

      (Cho et al., 2018)

      abnormal size | first instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal size | second instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal size | third instar larval stage, with Scer\GAL4en.PU

      (Zhang et al., 2015)

      abnormal size | third instar larval stage, with Scer\GAL4hh-Gal4

      (Mach et al., 2018)

      abnormal size | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      abnormal size | third instar larval stage, with Scer\GAL4ptc-559.1

      (Mach et al., 2018)

      hyperplasia, with Scer\GAL4GMR.PU

      (Liu et al., 2016)

      increased cell number | somatic clone, with Scer\GAL4NP5130

      (Ren et al., 2010)

      increased cell number | somatic clone | heat sensitive, with Scer\GAL4NP0001, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)

      increased cell number | somatic clone | heat sensitive, with Scer\GAL4NP5130, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)

      increased cell number | third instar larval stage, with Scer\GAL4repo.PU

      (Reddy and Irvine, 2011)

      increased mortality | adult stage | conditional, with Scer\GAL4hs.PB

      (Lee et al., 2018)

      increased mortality | adult stage | conditional, with Scer\GAL4NP0001

      (Lee et al., 2018)

      increased occurrence of cell division | adult stage | somatic clone, with Scer\GAL4NP5130

      (Ren et al., 2010)

      increased size | adult stage, with Scer\GAL4GMR.PU

      (Guo et al., 2020)

      increased size | adult stage, with Scer\GAL4ptc.PU

      (Guo et al., 2020)

      lethal | male limited, with Scer\GAL4Mef2.PR

      (Kaya-Çopur et al., 2021)

      lethal - all die before end of P-stage, with Scer\GAL4Bx-MS1096

      (Yu et al., 2013)

      lethal - all die before end of pupal stage, with Scer\GAL4Mef2.PR

      (Kaya-Çopur et al., 2021)

      visible, with Scer\GAL4GMR.PU

      (Liu et al., 2016, Qing et al., 2014)

      visible | adult stage, with Scer\GAL4GMR.PF

      (Cho et al., 2018)

      visible | adult stage, with Scer\GAL4GMR.PU

      (Guo et al., 2020)

      visible | adult stage, with Scer\GAL4ptc.PU

      (Guo et al., 2020)
      Phenotype Manifest In

      adult midgut | conditional, with Scer\GAL4hs.PB

      (Lee et al., 2018)

      anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4ptc-559.1

      (Mach et al., 2018)

      autophagosome | adult stage | conditional, with Scer\GAL4hs.PB

      (Lee et al., 2018)

      eye, with Scer\GAL4GMR.PF

      (Cho et al., 2018)

      eye, with Scer\GAL4GMR.PU

      (Guo et al., 2020, Liu et al., 2016, Qing et al., 2014)

      eye disc | third instar larval stage, with Scer\GAL4repo.PU

      (Reddy and Irvine, 2011)

      first basal wing cell, with Scer\GAL4ptc.PU

      (Guo et al., 2020)

      intestinal stem cell | somatic clone, with Scer\GAL4NP0001, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)

      intestinal stem cell | somatic clone, with Scer\GAL4NP5130

      (Ren et al., 2010)

      intestinal stem cell | somatic clone, with Scer\GAL4NP5130, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)

      larval brain | third instar larval stage, with Scer\GAL4repo.PU

      (Reddy and Irvine, 2011)

      larval central nervous system | first instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      larval central nervous system | second instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      larval central nervous system | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      larval glial cell | third instar larval stage, with Scer\GAL4repo.PU

      (Reddy and Irvine, 2011)

      lipid droplet | adult stage | conditional, with Scer\GAL4hs.PB

      (Lee et al., 2018)

      nucleus | pupal stage, with Scer\GAL4Mef2.PR

      (Kaya-Çopur et al., 2021)

      ommatidium | adult stage, with Scer\GAL4ey.PH

      (Brittle et al., 2012)

      type I neuroblast | first instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      type I neuroblast | second instar larval stage, with Scer\GAL4insc-Mz1407

      (Poon et al., 2016)

      wing disc posterior compartment | third instar larval stage, with Scer\GAL4en.PU

      (Zhang et al., 2015)

      wing disc posterior compartment | third instar larval stage, with Scer\GAL4hh-Gal4

      (Mach et al., 2018)
      Detailed Description
      Statement
      Reference

      The expression of wtsKK101055 under the control of Scer\GAL4Mef2.PR leads to indirect flight muscle atrophy during pupal development after 32h APF.

      (Kaya-Çopur et al., 2021)

      Expressing wtsKK101055 under the control of Scer\GAL4ptc.PU results in a reduced area between L3 and L4 in the wing.

      Expression under the control of Scer\GAL4GMR.PU leads to an enlarged eye.

      (Guo et al., 2020)

      Expression of wtsKK101055 under the control of Scer\GAL4GMR.PF leads to an increase in eye size when compared to controls.

      (Cho et al., 2018)

      The expression of wtsKK101055, driven by Scer\GAL4NP0001 or driven by Scer\GAL4hs.PB and induced by 29[o]C, but not when driven by Scer\GAL4ppl.PU, induces a significantly higher mortality to Ecc15 enteric infection, as compared to controls; Scer\GAL4hs.PB-driven expression induced by shift to 29[o]C for 2 days before the experiment abolishes the Ecc15 infection-induced lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut observed in controls.

      (Lee et al., 2018)

      Expressing wtsKK101055 under the control of Scer\GAL4ptc-559.1 stimulates tissue overgrowth in the wing disc and results in a significant expansion of the anterior-posterior compartment boundary of the disc, while expression under the control of Scer\GAL4hh-Gal4 causes an expansion of the wing disc posterior compartment in third instar larvae.

      (Mach et al., 2018)

      Expression of wtsKK101055 driven in the fat body of male flies by Scer\GAL4r4 (or driven by Scer\GAL4yolk in female fat bodies) significantly decreases survival of flies in response to Gram-positive bacteria (Staphylococcus aureus or Enterococcus faecalis) and a fungus (Aspergillus fumigatus), compared to controls; expression with Scer\GAL4r4 in males has no effect on survival in response to Gram-negative bacteria (Erwinia carotovora carotovora 15 or Escherichia coli). Bacterial load (measured as colony forming units 24hr post infection) of S. aureus (but not Erwinia carotovora carotovora 15) significantly increases in adult flies, compared to controls. Expression of wtsKK101055 driven by Scer\GAL4GMR.PU causes overgrowth of the eyes (leading to larger eyes).

      (Liu et al., 2016)

      Expressing wtsKK101055 under the control of Scer\GAL4insc-Mz1407 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in premature type I neuroblast reactivation in late-first/early-second instar larval ventral nerve cords and brains, concomitant with an overall volume increase in the central nervous system. A significant volume increase of the central nervous system is also visible at third instar larval stage.

      Expressing wtsKK101055 under the control of Scer\GAL4wor.PA in combination with Scer\GAL80ase.PN (to restrict expression to type II neuroblasts) and a Dicer-2 transgene (to enhance RNAi efficiency) has no effect on the volume of type II lineages in the brain lobe.

      Type I neuroblasts in somatic clones expressing wtsKK101055 under the control of Scer\GAL4NP1316 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) maintain cell polarity typical of wild-type controls.

      (Poon et al., 2016)

      Expression of wtsKK101055 under the control of Scer\GAL4en.PU in third instar larvae leads to overgrowth of the wing disc posterior compartment, as compared to controls.

      (Zhang et al., 2015)

      Expression of wtsKK101055 under the control of Scer\GAL4GMR.PU leads to increased eye size.

      (Qing et al., 2014)

      Expression of wtsKK101055 (co-expressed with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4repo.PU substantially increases glial cell numbers in the third instar larval eye disc and brain.

      (Reddy and Irvine, 2011)

      Expression of wtsKK101055 in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 increases the precursor cell population.

      After shifting adult flies to 29[o]C for 14 days, Scer\GAL80ts.αTub84B-Scer\GAL4esg-NP5130 driven wtsKK101055 flies exhibit a dramatic increase in the number of intestine stem cells in the midgut.

      Knockdown of wts through expression of wtsKK101055 in absorptive enterocytes in the adult midgut under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B induces a dramatic increase in intestine stem cell proliferation.

      (Ren et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, wtsKK101055 has increased size | adult stage phenotype, suppressible | partially by Rox8EY02351, Scer\GAL4GMR.PU

      (Guo et al., 2020)

      Scer\GAL4GMR.PU, wtsKK101055 has visible | adult stage phenotype, suppressible | partially by Rox8EY02351, Scer\GAL4GMR.PU

      (Guo et al., 2020)

      Scer\GAL4ptc.PU, wtsKK101055 has visible | adult stage phenotype, suppressible by Rox8EY02351, Scer\GAL4ptc.PU

      (Guo et al., 2020)

      Scer\GAL4ptc.PU, wtsKK101055 has increased size | adult stage phenotype, suppressible by Rox8EY02351, Scer\GAL4ptc.PU

      (Guo et al., 2020)

      Scer\GAL4GMR.PF, wtsKK101055 has abnormal size | adult stage phenotype, suppressible by Prp4kUAS.GFP, Scer\GAL4GMR.PF

      (Cho et al., 2018)

      Scer\GAL4GMR.PF, wtsKK101055 has visible | adult stage phenotype, suppressible by Prp4kUAS.GFP, Scer\GAL4GMR.PF

      (Cho et al., 2018)

      Scer\GAL4hs.PB, wtsKK101055 has increased mortality | adult stage | conditional phenotype, suppressible by ACCKK102082, Scer\GAL4hs.PB

      (Lee et al., 2018)

      Scer\GAL4hs.PB, wtsKK101055 has abnormal immune response | adult stage phenotype, suppressible by ACCKK102082, Scer\GAL4hs.PB

      (Lee et al., 2018)

      Scer\GAL4hs.PB, wtsKK101055 has increased mortality | adult stage | conditional phenotype, suppressible by bmmUAS.cUa/Scer\GAL4hs.PB

      (Lee et al., 2018)

      Scer\GAL4hs.PB, wtsKK101055 has abnormal immune response | adult stage phenotype, suppressible by bmmUAS.cUa/Scer\GAL4hs.PB

      (Lee et al., 2018)

      Scer\GAL4hs.PB, wtsKK101055 has increased mortality | adult stage | conditional phenotype, suppressible by G6pdUAS.cLa/Scer\GAL4hs.PB

      (Lee et al., 2018)

      Scer\GAL4hs.PB, wtsKK101055 has abnormal immune response | adult stage phenotype, suppressible by G6pdUAS.cLa/Scer\GAL4hs.PB

      (Lee et al., 2018)

      Scer\GAL4ptc-559.1, wtsKK101055 has abnormal size | third instar larval stage phenotype, suppressible by Hrb27CGD6964, Scer\GAL4ptc-559.1

      (Mach et al., 2018)

      Scer\GAL4ptc-559.1, wtsKK101055, ykiUAS.cOa.Tag:V5 has abnormal size | third instar larval stage phenotype, suppressible by Hrb27CGD6964, Scer\GAL4ptc-559.1

      (Mach et al., 2018)

      Scer\GAL4GMR.PU, wtsKK101055 has visible phenotype, suppressible by Ncoa6HMS00664, Scer\GAL4GMR.PU

      (Qing et al., 2014)

      Scer\GAL4Bx-MS1096, wtsKK101055 has lethal - all die before end of P-stage phenotype, suppressible by Hsap\LATS1UAS.cYa, Scer\GAL4Bx-MS1096

      (Yu et al., 2013)

      Scer\GAL4Bx-MS1096, wtsKK101055 has lethal - all die before end of P-stage phenotype, suppressible by Hsap\LATS1V719I.UAS, Scer\GAL4Bx-MS1096

      (Yu et al., 2013)

      Scer\GAL4Bx-MS1096, wtsKK101055 has lethal - all die before end of P-stage phenotype, suppressible by Hsap\LATS2UAS.cYa, Scer\GAL4Bx-MS1096

      (Yu et al., 2013)

      Scer\GAL4Bx-MS1096, wtsKK101055 has lethal - all die before end of P-stage phenotype, suppressible | partially by Hsap\LATS2G909R.UAS, Scer\GAL4Bx-MS1096

      (Yu et al., 2013)

      Scer\GAL4Bx-MS1096, wtsKK101055 has lethal - all die before end of P-stage phenotype, suppressible | partially by Hsap\LATS2C953.UAS, Scer\GAL4Bx-MS1096

      (Yu et al., 2013)

      Scer\GAL4NP5130, Scer\GAL80ts.αTub84B, wtsKK101055 has increased cell number | somatic clone | heat sensitive phenotype, suppressible by ykiRNAi.N.UAS/Scer\GAL4NP5130, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)

      Scer\GAL4NP0001, Scer\GAL80ts.αTub84B, wtsKK101055 has increased cell number | somatic clone | heat sensitive phenotype, suppressible by Scer\GAL4NP0001/ykiRNAi.N.UAS, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)

      Scer\GAL4NP5130, wtsKK101055 has increased cell number | somatic clone phenotype, suppressible by Scer\GAL4NP5130, sdRNAi.C.UAS/sdRNAi.N.UAS

      (Ren et al., 2010)

      Scer\GAL4NP5130, wtsKK101055 has increased occurrence of cell division | somatic clone | adult stage phenotype, suppressible by Scer\GAL4NP5130, sdRNAi.C.UAS/sdRNAi.N.UAS

      (Ren et al., 2010)

      Scer\GAL4NP5130, wtsKK101055 has increased cell number | somatic clone phenotype, suppressible by ykiRNAi.N.UAS/Scer\GAL4NP5130

      (Ren et al., 2010)

      Scer\GAL4NP5130, wtsKK101055 has increased occurrence of cell division | somatic clone | adult stage phenotype, suppressible by ykiRNAi.N.UAS/Scer\GAL4NP5130

      (Ren et al., 2010)

      Scer\GAL4NP0001, wtsKK101055 has increased occurrence of cell division | somatic clone | adult stage phenotype, suppressible by Scer\GAL4NP0001/ykiRNAi.N.UAS

      (Ren et al., 2010)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, wtsKK101055 has visible phenotype, non-suppressible by trrHMS01019/Scer\GAL4GMR.PU

      (Qing et al., 2014)
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, wtsKK101055 has eye phenotype, suppressible | partially by Rox8EY02351, Scer\GAL4GMR.PU

      (Guo et al., 2020)

      Scer\GAL4ptc.PU, wtsKK101055 has first basal wing cell phenotype, suppressible by Rox8EY02351, Scer\GAL4ptc.PU

      (Guo et al., 2020)

      Scer\GAL4GMR.PF, wtsKK101055 has eye phenotype, suppressible by Prp4kUAS.GFP, Scer\GAL4GMR.PF

      (Cho et al., 2018)

      Scer\GAL4ptc-559.1, wtsKK101055 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by Hrb27CGD6964, Scer\GAL4ptc-559.1

      (Mach et al., 2018)

      Scer\GAL4ptc-559.1, wtsKK101055, ykiUAS.cOa.Tag:V5 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by Hrb27CGD6964, Scer\GAL4ptc-559.1

      (Mach et al., 2018)

      Scer\GAL4GMR.PU, wtsKK101055 has eye phenotype, suppressible by Ncoa6HMS00664, Scer\GAL4GMR.PU

      (Qing et al., 2014)

      Scer\GAL4NP5130, wtsKK101055 has intestinal stem cell | somatic clone phenotype, suppressible by Scer\GAL4NP5130, sdRNAi.C.UAS/sdRNAi.N.UAS

      (Ren et al., 2010)

      Scer\GAL4NP5130, wtsKK101055 has intestinal stem cell | somatic clone phenotype, suppressible by ykiRNAi.N.UAS/Scer\GAL4NP5130

      (Ren et al., 2010)

      Scer\GAL4NP5130, Scer\GAL80ts.αTub84B, wtsKK101055 has intestinal stem cell | somatic clone phenotype, suppressible by ykiRNAi.N.UAS/Scer\GAL4NP5130, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)

      Scer\GAL4NP0001, Scer\GAL80ts.αTub84B, wtsKK101055 has intestinal stem cell | somatic clone phenotype, suppressible by Scer\GAL4NP0001/ykiRNAi.N.UAS, Scer\GAL80ts.αTub84B

      (Ren et al., 2010)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, wtsKK101055 has eye phenotype, non-suppressible by trrHMS01019/Scer\GAL4GMR.PU

      (Qing et al., 2014)
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of pnutGD1512 and wtsKK101055 from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts] leads to the formation of tumors in the third instar larval wing disc.

      (Gerlach et al., 2018)

      Expression of Ncoa6HMS00664 suppresses the increase in eye size seen when wtsKK101055 is expressed under the control of Scer\GAL4GMR.PU.

      Expression of trrHMS01019 does not visibly suppress the increase in eye size seen when wtsKK101055 is expressed under the control of Scer\GAL4GMR.PU.

      (Qing et al., 2014)

      Co-expression of ykidsRNA.N.Scer\UAS with wtsKK101055 in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 suppresses the increase in the number of precursor cells.

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with wtsKK101055 in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 suppresses the increase in the number of precursor cells found in wtsKK101055 mutants.

      Co-expression of ykidsRNA.N.Scer\UAS in flies driving wtsKK101055 under the control of Scer\GAL80ts.αTub84B-Scer\GAL4esg-NP5130 that have been shifted to 29[o]C for 14 days suppresses the dramatic increase seen in the number of intestine stem cells found upon expression of wtsKK101055 alone.

      Co-expression of ykidsRNA.N.Scer\UAS with wtsKK101055 in absorptive enterocytes in the adult midgut under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B suppresses the proliferation increase in intestine stem cells seen upon expression of wtsKK101055 alone.

      (Ren et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsap\LATS2G909R.Scer\UAS and wtsKK101055 both under the control of Scer\GAL4Bx-MS1096 results in wings that are about 11% larger than wild-type wings.

      Co-expression of Hsap\LATS2C953.Scer\UAS and wtsKK101055 both under the control of Scer\GAL4Bx-MS1096 results in abnormal wing morphology.

      (Yu et al., 2013)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      WtsV106174
      (Cho et al., 2020)
      wtsKK101055
      (Forest et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (41)