The expression of wtsKK101055, driven by Scer\GAL4NP0001 or driven by Scer\GAL4hs.PB and induced by 29[o]C, but not when driven by Scer\GAL4ppl.PU, induces a significantly higher mortality to Ecc15 enteric infection, as compared to controls; Scer\GAL4hs.PB-driven expression induced by shift to 29[o]C for 2 days before the experiment abolishes the Ecc15 infection-induced lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut observed in controls.
Expressing wtsKK101055 under the control of Scer\GAL4ptc-559.1 stimulates tissue overgrowth in the wing disc and results in a significant expansion of the anterior-posterior compartment boundary of the disc, while expression under the control of Scer\GAL4hh-Gal4 causes an expansion of the wing disc posterior compartment in third instar larvae.
Expression of wtsKK101055 driven in the fat body of male flies by Scer\GAL4r4 (or driven by Scer\GAL4yolk in female fat bodies) significantly decreases survival of flies in response to Gram-positive bacteria (Staphylococcus aureus or Enterococcus faecalis) and a fungus (Aspergillus fumigatus), compared to controls; expression with Scer\GAL4r4 in males has no effect on survival in response to Gram-negative bacteria (Erwinia carotovora carotovora 15 or Escherichia coli). Bacterial load (measured as colony forming units 24hr post infection) of S. aureus (but not Erwinia carotovora carotovora 15) significantly increases in adult flies, compared to controls. Expression of wtsKK101055 driven by Scer\GAL4GMR.PU causes overgrowth of the eyes (leading to larger eyes).
Expressing wtsKK101055 under the control of Scer\GAL4insc-Mz1407 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in premature type I neuroblast reactivation in late-first/early-second instar larval ventral nerve cords and brains, concomitant with an overall volume increase in the central nervous system. A significant volume increase of the central nervous system is also visible at third instar larval stage.
Expressing wtsKK101055 under the control of Scer\GAL4wor.PA in combination with Scer\GAL80ase.PN (to restrict expression to type II neuroblasts) and a Dicer-2 transgene (to enhance RNAi efficiency) has no effect on the volume of type II lineages in the brain lobe.
Type I neuroblasts in somatic clones expressing wtsKK101055 under the control of Scer\GAL4NP1316 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) maintain cell polarity typical of wild-type controls.
Expression of wtsKK101055 under the control of Scer\GAL4en.PU in third instar larvae leads to overgrowth of the wing disc posterior compartment, as compared to controls.