FlyBase Allele Report: Dmel\hts[KK101849]

General Information

Symbol

Dmel\hts^KK101849

Species

D. melanogaster

Name

FlyBase ID

FBal0231517

Feature type

allele

Associated gene

Dmel\hts

Associated Insertion(s)

Carried in Construct

P{KK101849}

Also Known As

hts^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101849}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101849

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cerebral palsy

CEA

(Kruer et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^Appl.G1a

(Kruer et al., 2013)

abnormal neuroanatomy, with Scer\GAL4²²¹

(Hattori et al., 2013)

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

decreased cell number | progressive, with Scer\GAL4^Ugt36A1.PU

(Gonçalves et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing hts^KK101849 under the control of Scer\GAL4^Ugt36A1.PU results in a progressive decrease in the number of adult pericardial nephrocytes. Expression under the control of Scer\GAL4^tin.PU does not induce obvious cardiac defects (heart period, cardiac output, arrhythmia index, diastolic and systolic diameter, and fractional shortening).

Larval garland nephrocytes expressing hts^KK101849 under the control of Scer\GAL4^pros.PMG frequently show a discontinuous slit diaphragm.

(Gonçalves et al., 2018)

The expression of hts^KK101849 under the control of Scer\GAL4^c564 has no obvious affect on the inflammatory response to Gram-positive bacterial infection or under basal conditions, as compared to controls.

(Kumar et al., 2016)

The expression of hts^KK101849 under the control of Scer\GAL4²²¹, in combination with Dicer-2 for efficient RNAi, results in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons exhibiting dendrite branch misrouting, as compared to controls; expression under the control of Scer\GAL4^ppk.PG, in combination with Dicer-2 for efficient RNAi, results in third instar larval class IV abdominal dorsal multidendritic ddaC neurons exhibiting abnormal dendritic terminals, as compared to controls.

(Hattori et al., 2013)

Scer\GAL4^Appl.G1a-mediated expression of hts^GD14187 results in locomotion deficits in the fast phototaxis assay.

(Kruer et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Ugt36A1.PU, hts^KK101849 has decreased cell number | progressive phenotype, enhanceable by Gcn5^HMS00941, Scer\GAL4^Ugt36A1.PU