Expression of MED27KK102849 under the control of Scer\GAL4bam.T:Hsim\VP16 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in partial meiotic arrest in spermatocytes.
Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN prolongs the lifetime and prevents shrinkage of pupal neuroblasts (NBs) without having any obvious effects on larval NBs. These longer lived NBs continue dividing for a longer time and generate significantly more neuronal progeny than wild type.
Cultured pupal neuroblasts (NBs) from flies expressing MED27KK102849 under the control of Scer\GAL4wor.PA and Scer\GAL80ase.PN do not reduce their net size (as wild type pupal NBs do) and are indistinguishable from larval NBs.
Expression via Scer\GAL4ey-OK107 prevents proper differentiation of alpha/beta neurons in mushroom bodies, resulting in a smaller volume.
Expression via Scer\GAL4insc-Mz1407 reduces the difference (increase) in oxygen consumption rates seen in wild type larval vs pupal brains. NADH levels are decreased, but overall energy/ATP levels are unchanged. Lactate levels are reduced in larvae and increased in pupae, effectively reducing the difference between the two stages, whereas lactate levels strongly decrease from larval to pupal stages in wild type.