FlyBase Allele Report: Dmel\Rab5[KK102895]

General Information

Symbol

Dmel\Rab5^KK102895

Species

D. melanogaster

Name

FlyBase ID

FBal0232077

Feature type

allele

Associated gene

Dmel\Rab5

Associated Insertion(s)

Carried in Construct

P{KK102895}

Also Known As

Rab5 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK102895}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102895

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut region R3, with Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

adult midgut region R5, with Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

cyst cell of testis, with Scer\GAL4^C587

(Zohar-Fux et al., 2022)

dendritic tree | P-stage, with Scer\GAL4^ppk.PU

(Zhang et al., 2014)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Scer\GAL4^grh.D4

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Scer\GAL4^grh.D4

(Wingen et al., 2017)

epithelium, with Scer\GAL4^NP1624

(Berns et al., 2014)

female germline cyst, with Scer\GAL4^NP1624

(Berns et al., 2014)

larval dorsal multidendritic neuron ddaC | P-stage, with Scer\GAL4^ppk.PU

(Zhang et al., 2014)

lipid droplet | adult stage, with Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

lysosome | decreased number | spermatogenesis, with Scer\GAL4^C587

(Zohar-Fux et al., 2022)

nephrocyte | larval stage, with Scer\GAL4^Cubn2.PA

(Atienza-Manuel et al., 2021)

nephrocyte diaphragm | larval stage, with Scer\GAL4^Cubn2.PA

(Atienza-Manuel et al., 2021)

nucleus | larval stage, with Scer\GAL4^Cubn2.PA

(Atienza-Manuel et al., 2021)

spermatogonium | increased number, with Scer\GAL4^C587

(Zohar-Fux et al., 2022)

Detailed Description

Statement

Reference

The expression of Rab5^KK102895 under the control of Scer\GAL4^Cubn2.PA leads to larval nephrocytes showing decreased density of slit diaphragms in the cortical region and abnormal internal accumulation of slit diaphragms, compared to controls.

(Atienza-Manuel et al., 2021)

Expression of Rab5^KK102895 under the control of CtxGlia-SplitGal4 (Hsim\VP16^AD.nrv2 and Scer\GAL4^{DBD.wrapper.932i}) leads to an altered cortex glial morphology in third instar larvae when compared to controls.

(Coutinho-Budd et al., 2017)

Animals expressing Rab5^KK102895 under the control of Scer\GAL4^grh.D4 are lethal and show defects in larval dorsal trunk elongation (resulting in internalization of posterior spiracles into the body cavity).

(Wingen et al., 2017)

Expression of Rab5^KK102895 under the control of Scer\GAL4^Hml.Δ does not result in any significantly reduced survival when infected with Leishmania amastigotes, as compared to wild type.

(Okuda et al., 2016)

Scer\GAL4^tj-NP1624-mediated expression results in the transformation of the normal germline cyst epithelial monolayer into two or more cell layers.

(Berns et al., 2014)

Primary macrophages isolated from animals expressing Rab5^KK102895 under the control of Scer\GAL4^Pxn.PS have an similar number of F-actin rich protrusions to wild type.

(Jean et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rab5^KK102895, Scer\GAL4^{repo.Switch.2301} is an enhancer of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103945

P{KK102895}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rab5^KK102895

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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