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General Information
Symbol
Dmel\Ets21CKK103211
Species
D. melanogaster
Name
FlyBase ID
FBal0232230
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ets21cRNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Ets21CKK103211 under the control of Scer\GAL4esg.PU, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, prevents the increased cell division seen in controls in response to oxidative stress (paraquat) and significantly reduces survival of females, but not males, under these conditions; expression under normal conditions significantly reduces intestinal stem cell division and reduces epithelial turnover, compared to controls, with no loss of precursor cells, and suppresses aging-induced effects in the guts of older flies such as precursor cell accumulation and dysplasia, compared to controls.

Expression of Ets21CKK103211 under the control of Scer\GAL4NP0001, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, prevents the elimination of enterocytes seen in controls in response to oxidative stress (paraquat) and significantly reduces survival of females, but not males, under these conditions; expression under normal conditions prevents aging-associated loss of enterocytes and gut remodelling in older flies, compared to controls; in 10 day old flies, the posterior midgut is significantly reduced in diameter and there are fewer apoptotic enterocytes, compared to controls.

Expression of Ets21CKK103211 RNAi under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of RNAi expression) results in a suppression of regeneration-associated proliferation of intestinal stem cells in the adult midgut upon Pseudomonas entomophila enteric infection.

Expression of Ets21CKK103211 under the control of Scer\GAL4Act.PU in clones in the eye-antennal disc does not result in any significant defect in differences in number or morphology of clones, eye-antennal disc morphology, photoreceptor differentiation, or adult eye development, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Third instar larval eye-antennal imaginal disc clones homozygous for scrib1 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU do not induce significant autophagosomes (assessed by a Atg8a fluorescence reporter) in neighboring disc cells if the clones also co-express both Ets21CKK103211 and kaydsRNA.Scer\UAS.cUa under the control of Scer\GAL4Act.PU.

Larvae with eye-antennal disc clones co-expressing Ets21CKK103211 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU exhibit developmental delay, a noticeable enlargement of clonal tissue that eventually outcompetes the non-clonal tissue, defective photoreceptor differentiation, and clonal tissue exhibits invasive behavior and infiltrates the brain lobes and the ventral nerve cord, as compared to controls.

Clonal co-expression of bskDN.Scer\UAS.cUa suppresses the invasiveness of clonal cells, but does not suppress tumor formation or developmental delay seen with eye-antennal disc clones co-expressing Ets21CKK103211 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU.

Clonal co-expression of Ets21CKK103211 partially suppresses the lethality and developmental delay seen in larvae with scrib1 mutant eye-antennal disc clones expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU, but fails to suppress the invasive infiltration of the ventral nerve cord exhibited by cells of these clones.

Larvae with scrib1, kay3 mutant eye-antennal disc clones expressing Ras85DV12.Scer\UAS and Ets21CKK103211 under the control of Scer\GAL4Act.PU exhibit suppression of larval lethality as compared to either larvae with scrib1, kay3 mutant eye-antennal disc clones expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU, or those with scrib1 mutant eye-antennal disc clones expressing Ras85DV12.Scer\UAS and Ets21CKK103211 under the control of Scer\GAL4Act.PU; but these larvae still exhibit pupal lethality.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Ets21CKK103211
ets21c<up>LONG RNAi</up>
Name Synonyms
Secondary FlyBase IDs
    References (6)