Expression of Cep89^KK103260 under the control of Scer\GAL4^Act5C.PI (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) at 28[o]C results in complete lethality at a late pupal stage.

Expression of Cep89^KK103260 under the control of Scer\GAL4^Mef2.PR results in complete lethality at a late pupal stage at temperatures ranging from 20 to 28[o]C.

Expression of Cep89^KK103260 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) at 28[o]C is mostly lethal. The few adults escapers are weak and die shortly after eclosion.

Expression of Cep89^KK103260 under the control of Scer\GAL4^elav.PLu at 26.5[o]C results in partial lethality.

Muscle area at the neuromuscular junction is significantly decreased compared to wild type in larvae expressing Cep89^KK103260 under the control of Scer\GAL4^elav.PLu.

Expression of Cep89^KK103260 under the control of Scer\GAL4^Bx-MS1096 results in a dramatic reduction in wing size.

The regular array of the ommatidia is disrupted in flies expressing Cep89^KK103260 under the control of Scer\GAL4^GMR.PF (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) and the surfaces of the ommatidia are collapsed. The rhabdomeres are malformed and smaller than normal and vacuoles are seen in the cytoplasm of photoreceptors. Photoreceptor neurons show mitochondrial defects, with the overall mitochondrial structure being affected in more than 50% of mitochondria, and large empty spaces and vacuoles being seen. The 'on' and 'off' transients of the electroretinogram are strongly reduced and membrane depolarization is nearly absent.

Flies expressing Cep89^KK103260 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) habituate slower than wild type in a light-off jump reflex habituation paradigm, maintaining a higher jump response throughout the entire course of the experiment.