FlyBase Allele Report: Dmel\Tsf1[KK103815]

General Information

Symbol

Dmel\Tsf1^KK103815

Species

D. melanogaster

Name

FlyBase ID

FBal0232543

Feature type

allele

Associated gene

Dmel\Tsf1

Associated Insertion(s)

Carried in Construct

P{KK103815}

Also Known As

Tsf1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK103815}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK103815

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Friedreich ataxia 1

modeled by fh^RNAi.UAS.cAa

(Soriano et al., 2016)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Xia et al., 2024, Xue et al., 2022)

modeled by Pink1^RNAi.UAS.cYa

(Xue et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

majority die during pupal stage | nutrition conditional, with Scer\GAL4^Act5C.PI

(Xiao et al., 2019)

partially lethal, with Scer\GAL4^Act5C.PI

(Weber et al., 2022)

partially lethal - majority die | nutrition conditional, with Scer\GAL4^Act5C.PI

(Xiao et al., 2019)

partially lethal - majority live, with Scer\GAL4^Act5C.PI

(Xiao et al., 2019)

partially lethal - majority live, with Scer\GAL4^Cg.PA

(Xiao et al., 2019)

partially lethal - majority live, with Scer\GAL4^da.G32

(Xiao et al., 2019)

partially lethal - majority live | nutrition conditional, with Scer\GAL4^da.G32

(Xiao et al., 2019)

short lived | nutrition conditional, with Scer\GAL4^NP3084

(Xiao et al., 2019)

some die during P-stage, with Scer\GAL4^Act5C.PI

(Weber et al., 2022)

some die during pupal stage, with Scer\GAL4^Act5C.PI

(Xiao et al., 2019)

some die during pupal stage, with Scer\GAL4^Cg.PA

(Xiao et al., 2019)

viable, with Scer\GAL4^NP3084

(Xiao et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing Tsf1^KK103815 under the control of Scer\GAL4^Act5C.PI leads to a significantly lower eclosion rate in flies regardless of iron or zinc content in their diet. Expression under the control of either Scer\GAL4^Act5C.PI or Scer\GAL4^da.G32 results in a higher frequency of pupal lethality when flies are fed an iron-rich diet compared to flies fed a regular diet and wild-type controls (regardless of diet). Mortality during development is increased in flies fed a regular diet, but unaffected under an iron-rich diet when expression is under the control of Scer\GAL4^da.G32.

Expressing Tsf1^KK103815 under the control of Scer\GAL4^NP3084 results in a severely shortened lifespan in adults fed an iron-rich diet (but not a regular or iron-depleted diet) compared to matched controls.

Expressing Tsf1^KK103815 under the control of Scer\GAL4^NP3084, Scer\GAL4^NP5156 or Scer\GAL4^elav-C155 has no impact on mortality during development under regular dietary conditions. Expression under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^GMR.PFa has no impact on eye morphology.

(Xiao et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU, Tsf1^KK103815 has visible | adult stage phenotype, enhanceable by mfrn^NIG.4963R, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Scer\GAL4^da.G32, Tsf1^KK103815 has partially lethal - majority live phenotype, enhanceable by Fer1HCH^GD4853, Scer\GAL4^da.G32

(Xiao et al., 2019)

Scer\GAL4^Cg.PA, Tsf1^KK103815 has partially lethal - majority live phenotype, enhanceable by Fer1HCH^GD4853, Scer\GAL4^Cg.PA

(Xiao et al., 2019)

NOT Enhancer of

Statement

Reference

mfrn^NIG.4963R/Tsf1^KK103815, Scer\GAL4^Mhc.PU is a non-enhancer of abnormal jumping phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Tsf1^KK103815 is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ple.PF/Tsf1^KK103815 is a suppressor | partially of decreased rate of jump response phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ple.PF/Tsf1^KK103815 is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Xia et al., 2024)

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a suppressor | partially of visible | adult stage phenotype of Pink1^B9

(Xue et al., 2022)

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a suppressor | partially of abnormal jumping phenotype of Pink1^B9

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a suppressor of visible | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a suppressor of abnormal jumping phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^NP3084 is a suppressor | partially of increased mortality during development phenotype of Fer1HCH^GD4853, Scer\GAL4^NP3084

(Xiao et al., 2019)

Tsf1^KK103815, Scer\GAL4^NP3084 is a suppressor | partially of lethal | nutrition conditional phenotype of Fer1HCH^GD4853, Scer\GAL4^NP3084

(Xiao et al., 2019)

Tsf1^KK103815, Scer\GAL4^da.G32 is a suppressor | partially of lethal - all die during second instar larval stage phenotype of Fer1HCH^GD4853, Scer\GAL4^da.G32

(Xiao et al., 2019)

Scer\GAL4^da.G32/Tsf1^KK103815 is a suppressor | partially of lethal - all die during second instar larval stage phenotype of Fer1HCH^GD4853, Scer\GAL4^Cg.PA

(Xiao et al., 2019)

Scer\GAL4^NP3084/Tsf1^KK103815 is a suppressor of partially lethal - majority die | nutrition conditional phenotype of Scer\GAL4^NP3084, Zip99C^GD370

(Xiao et al., 2019)

NOT Suppressor of

Statement

Reference

mfrn^NIG.4963R/Tsf1^KK103815, Scer\GAL4^Mhc.PU is a non-suppressor of abnormal jumping phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a non-suppressor of visible | adult stage phenotype of park^Δ21/park¹

(Xue et al., 2022)

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a non-suppressor of abnormal jumping phenotype of park^Δ21/park¹

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^elav-C155 is a non-suppressor of partially lethal - majority die phenotype of Fer1HCH^GD4853, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Tsf1^KK103815, Scer\GAL4^elav-C155 is a non-suppressor of visible | adult stage phenotype of Fer1HCH^GD4853, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Tsf1^KK103815, Scer\GAL4^elav-C155 is a non-suppressor of abnormal size | adult stage phenotype of Fer1HCH^GD4853, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Tsf1^KK103815, Scer\GAL4^GMR.PFa is a non-suppressor of visible | adult stage phenotype of Fer1HCH^GD4853, Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

Tsf1^KK103815, Scer\GAL4^GMR.PFa is a non-suppressor of abnormal size | adult stage phenotype of Fer1HCH^GD4853, Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

Other

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^NP3084, Tsf1^KK103815 has partially lethal - majority die phenotype

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^da.G32, Tsf1^KK103815 has partially lethal - majority die phenotype

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^Cg.PA, Scer\GAL4^da.G32, Tsf1^KK103815 has partially lethal - majority die phenotype

(Xiao et al., 2019)

Scer\GAL4^NP3084, Tsf1^KK103815, Zip99C^GD370 has viable phenotype

(Xiao et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU, Tsf1^KK103815 has wing phenotype, enhanceable by mfrn^NIG.4963R, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU, Tsf1^KK103815 has adult thorax phenotype, enhanceable by mfrn^NIG.4963R, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Tsf1^KK103815 is a suppressor | partially of adult dopaminergic neuron | decreased number phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ple.PF/Tsf1^KK103815 is a suppressor | partially of adult brain phenotype of Pink1^B9

(Xia et al., 2024)

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a suppressor | partially of wing phenotype of Pink1^B9

(Xue et al., 2022)

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a suppressor of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a suppressor of adult thorax phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a suppressor of adult somatic muscle cell of thorax phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a suppressor of myotube | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

NOT Suppressor of

Statement

Reference

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a non-suppressor of adult thorax phenotype of park^Δ21/park¹

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a non-suppressor of indirect flight muscle cell phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^Mhc.PU is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Xue et al., 2022)

Tsf1^KK103815/Scer\GAL4^Mhc.PU is a non-suppressor of wing phenotype of park^Δ21/park¹

(Xue et al., 2022)

Tsf1^KK103815, Scer\GAL4^elav-C155 is a non-suppressor of eye phenotype of Fer1HCH^GD4853, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Tsf1^KK103815, Scer\GAL4^GMR.PFa is a non-suppressor of eye phenotype of Fer1HCH^GD4853, Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing Tsf1^KK103815 with Fer1HCH^GD4853 under the control of Scer\GAL4^NP3084 results in partial lethality (majority die) regardless of iron content in the diet.

Co-expressing Tsf1^KK103815 with Zip99C^GD370 under the control of Scer\GAL4^NP3084 results in a full rescue of lethality in flies fed an iron-depleted diet. Flies are viable also when fed a regular or iron-rich diet.

(Xiao et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106479

P{KK103815}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tsf1^KK103815

Tsf1^shRNA

(Soriano et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close