FlyBase Allele Report: Dmel\Dark[KK104215]

General Information

Symbol

Dmel\Dark^KK104215

Species

D. melanogaster

Name

FlyBase ID

FBal0232822

Feature type

allele

Associated gene

Dmel\Dark

Associated Insertion(s)

Carried in Construct

P{KK104215}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK104215}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK104215

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Dark^KK104215 under the control of Scer\GAL4^ap-md544 does not induce opaque or curly wings, which would be indicative of apoptosis or growth inhibition, respectively.

Expression under the control of Scer\GAL4^C10 does not affect the overall wing size.

(Shinoda et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of visible | adult stage phenotype of Dronc^UAS.cSa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of abnormal eye color phenotype of Dronc^UAS.cSa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215/Usp8^KK100733, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Dark^KK104215/Usp8^KK100733, Scer\GAL4^GMR.PFa is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of visible | adult stage phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of abnormal eye color phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor | partially of abnormal eye color phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor | partially of abnormal size | adult stage phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215/Scer\GAL4^GMR.PFa is a suppressor of visible | adult stage phenotype of grim^GMR.PC

(Sinenko, 2017)

Dark^KK104215/Scer\GAL4^GMR.PFa is a suppressor of abnormal eye color phenotype of grim^GMR.PC

(Sinenko, 2017)

Dark^KK104215/Scer\GAL4^GMR.PFa is a suppressor of abnormal size | adult stage phenotype of grim^GMR.PC

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of visible | adult stage phenotype of Dronc^{S130A.UAS.cSa}, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of abnormal eye color phenotype of Dronc^{S130A.UAS.cSa}, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Usp15-31^KK101035/Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Usp15-31^KK101035/Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Usp15-31^KK101035/Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Dark^KK104215/Usp8^KK100733, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, rpr^UAS.C

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Dronc^UAS.cSa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Dark^KK104215/Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of grim^GMR.PC

(Sinenko, 2017)

Dark^KK104215, Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Dronc^{S130A.UAS.cSa}, Scer\GAL4^GMR.PFa

(Sinenko, 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The eye defects characteristic for adult flies expressing any of the following: Dronc^UAS.cSa, Dronc^{S130A.UAS.cSa} and Dcp-1^UAS.cKa (photoreceptor depigmentation) or Diap1^{dsRNA.UAS.cLa} (depigmentation and size reduction) under the control of Scer\GAL4^GMR.PFa or those carrying one copy of grim^GMR.PC are all strongly improved by Scer\GAL4^GMR.PFa-driven expression of Dark^KK104215.

The small rough eyes observed in flies expressing rpr^Scer\UAS.C under the Scer\GAL4^GMR.PFa driver is only partially suppressed (the size is rescued but not the rough appearance) by co-expression of Dark^KK104215, however, when Dark^KK104215 is combined with Usp15-31^KK101035, it leads to a complete suppression. Combination of Dark^KK104215 with another transgene, Usp8^KK100733 on the other hand does not strengthen the rescue.

(Sinenko, 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v100405

P{KK104215}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ark^KK104215

Dark^KK104215

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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