FlyBase Allele Report: Dmel\caz[KK107486]

General Information

Symbol

Dmel\caz^KK107486

Species

D. melanogaster

Name

FlyBase ID

FBal0234963

Feature type

allele

Associated gene

Dmel\caz

Associated Insertion(s)

Carried in Construct

P{KK107486}

Also Known As

UAS-Caz-IR, UAS-Caz-IR_363-399

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK107486}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK107486

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Shimamura et al., 2014)

model of amyotrophic lateral sclerosis type 6

CEC

(Cacciottolo et al., 2019)

CEA

(Muraoka et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate spinocerebellar ataxia type 31

modeled by Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^W.UAS, Hsap\TARDBP^UAS.cIb

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS, Hsap\TARDBP^UAS.cIb

(Ishiguro et al., 2017)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

model of amyotrophic lateral sclerosis type 6

is exacerbated by Gem3^ΔN.UAS

(Cacciottolo et al., 2019)

is ameliorated by hpRNA:CR18854^RNAi.UAS.cUa

(Muraoka et al., 2018)

is ameliorated by hpRNA:CR18854^VSH33053

(Muraoka et al., 2018)

is ameliorated by lncRNA:Hsrω^RNAi.Sym.UAS

(Muraoka et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage, with Scer\GAL4^ey-OK107

(Machado Almeida et al., 2021)

abnormal locomotor behavior | adult stage, with Scer\GAL4^nSyb.PU, caz^HMS00790

(Frickenhaus et al., 2015)

abnormal locomotor behavior | adult stage | adult stage, with Scer\GAL4^elav.PLu

(Sasayama et al., 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PLu

(Sasayama et al., 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Xia et al., 2012)

increased cell death, with Scer\GAL4^GMR.PS

(Shimamura et al., 2014)

partially lethal - majority die | heat sensitive, with Scer\GAL4^Act5C.PU

(Xia et al., 2012)

partially lethal - majority die | heat sensitive, with Scer\GAL4^elav.PU

(Xia et al., 2012)

partially lethal - majority die | heat sensitive, with Scer\GAL4^Toll-6-D42

(Xia et al., 2012)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Xia et al., 2012)

visible | larval stage, with Scer\GAL4^GMR.PF

(Xia et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of caz^KK107486 under the control of Scer\GAL4^nSyb.PU does not cause developmental lethality or locomotor defects in a climbing assay. Co-expression of caz^KK107486 and caz^HMS00790 or caz^KK107486 and caz^HMS00156 under the control of Scer\GAL4^nSyb.PU does not cause developmental lethality, but co-expression of caz^KK107486 and caz^HMS00790 under the control of Scer\GAL4^nSyb.PU does result in modest climbing defects.

(Frickenhaus et al., 2015)

Knockdown of caz through the expression of caz^KK107486 in the developing eye in the region posterior to the morphogenetic furrow, under the control of Scer\GAL4^GMR.PS, induces morphologically aberrant rough eyes. SEM images show fusion of ommatidia and a lack of bristles.

caz knockdown eye discs (generated through expression of caz^KK107486 under the control of Scer\GAL4^GMR.PS) exhibit wild-type photoreceptor cells and neurons. There appears to be some increase in the occurrence of cone cell fusion. The orientation of ommatidia in these mutants can be irregular, and the size of ommatidia can vary. In addition, some ommatidia are apparently fused, suggesting that knockdown of caz disrupts differentiation of pupal ommatidial cell types, especially cone cells, and 1[o], 2[o], and 3[o] pigment cells.

(Shimamura et al., 2014)

Flies expressing caz^KK107486 under the control of Scer\GAL4^elav.PLu show no significant difference in life span compared to control flies. The flies show reduced ability in a climbing assay compared to controls.

The total length of synaptic branches and the number of boutons at the neuromuscular junction are reduced compared to wild type in larvae expressing caz^KK107486 under the control of Scer\GAL4^elav.PLu, while bouton size is normal.

(Sasayama et al., 2012)

Expression of caz^KK107486 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^Bx-MS1096 disrupts wing growth.

Ubiquitous expression of caz^KK107486 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^Act5C.PU reduces adult viability to approximately 30% at 25[o]C, but this is increased to approximately 60% when flies are raised at 19[o]C.

Expression of caz^KK107486 (co-expressed with Dcr-2^Scer\UAS.cDa) in neurons under the control of Scer\GAL4^elav.PU severely reduces adult viability to approximately 25% at 25[o]C. Viability is increased to approximately 80% when flies are raised at 19[o]C.

Expression of caz^KK107486 (co-expressed with Dcr-2^Scer\UAS.cDa) in neurons under the control of Scer\GAL4^D42 severely reduces adult viability to approximately 30% at 25[o]C. Viability is increased to approximately 75% when flies are raised at 19[o]C.

Expression of caz^KK107486 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^GMR.PF disrupts ommatidium formation in the larval eye disc.

Expression of caz^KK107486 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^VGlut-OK371 disrupts the arrangement of motor neurons in neuromeres A1-A5 of the third instar larval ventral nerve cord. Nuclear staining appears similar to wild type and no apoptosis is seen.

(Xia et al., 2012)

Down-regulation of caz through expression of caz^KK107486 in the developing eye under the control of Scer\GAL4^GMR.PF does not affect eye morphology. No locomotive defects are seen in flies expressing caz^KK107486 in motorneurons under the control of Scer\GAL4^VGlut-OK371.

(Chen et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, caz^KK107486 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Shimamura et al., 2014)

Enhancer of

Statement

Reference

caz^KK107486/Scer\GAL4^GMR.PS is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

caz^KK107486/Scer\GAL4^GMR.PS is an enhancer of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

NOT Suppressor of

Statement

Reference

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

caz^KK107486, Scer\GAL4^GMR.PS is a non-suppressor of abnormal size | adult stage phenotype of Hsap\TARDBP^UAS.cIb, Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

caz^KK107486, Scer\GAL4^GMR.PS is a non-suppressor of visible | adult stage phenotype of Hsap\TARDBP^UAS.cIb, Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, caz^KK107486 has eye phenotype, suppressible by rho^7M43/rho[+]

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has ommatidium phenotype, suppressible by rho^7M43/rho[+]

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has interommatidial bristle phenotype, suppressible by rho^7M43/rho[+]

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has cone cell phenotype, suppressible by rho^7M43/rho[+]

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has eye phenotype, suppressible by rho[+]/rho^AA69

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has ommatidium phenotype, suppressible by rho[+]/rho^AA69

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has interommatidial bristle phenotype, suppressible by rho[+]/rho^AA69

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has cone cell phenotype, suppressible by rho[+]/rho^AA69

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has eye phenotype, suppressible by Scer\GAL4^GMR.PS/rho^GD2243

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has ommatidium phenotype, suppressible by Scer\GAL4^GMR.PS/rho^GD2243

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has interommatidial bristle phenotype, suppressible by Scer\GAL4^GMR.PS/rho^GD2243

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has cone cell phenotype, suppressible by Scer\GAL4^GMR.PS/rho^GD2243

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has eye phenotype, suppressible by ru[+]/ru¹

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has ommatidium phenotype, suppressible by ru[+]/ru¹

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has interommatidial bristle phenotype, suppressible by ru[+]/ru¹

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has cone cell phenotype, suppressible by ru[+]/ru¹

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has eye phenotype, suppressible by mirr^SaiD3

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has ommatidium phenotype, suppressible by mirr^SaiD3

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has interommatidial bristle phenotype, suppressible by mirr^SaiD3

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has cone cell phenotype, suppressible by mirr^SaiD3

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has ommatidium phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has interommatidial bristle phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Shimamura et al., 2014)

Scer\GAL4^GMR.PS, caz^KK107486 has cone cell phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Shimamura et al., 2014)

Enhancer of

Statement

Reference

caz^KK107486/Scer\GAL4^GMR.PS is an enhancer of eye phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

caz^KK107486/Scer\GAL4^GMR.PS is an enhancer of embryonic/larval salivary gland | larval stage phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

caz^KK107486/Scer\GAL4^GMR.PS is an enhancer of eye disc phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

NOT Suppressor of

Statement

Reference

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

caz^KK107486/Scer\GAL4^GMR.PS is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

caz^KK107486, Scer\GAL4^GMR.PS is a non-suppressor of eye | adult stage phenotype of Hsap\TARDBP^UAS.cIb, Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

A rho^7M43 or rho^AA69 heterozygous background significantly suppresses the caz^KK107486-induced rough eye phenotype and rescues the fusion of cone cells in pupal retinae.

Knockdown of rho, through expression of rho^GD2243, suppresses the rough eye phenotype induced by expression of caz^KK107486.

A ru¹ background suppresses the rough eye phenotype induced by expression of caz^KK107486.

A mirr^SaiD3 background suppresses the rough eye phenotype induced by expression of caz^KK107486.

(Shimamura et al., 2014)

Xenogenetic Interactions

Statement

Reference

The co-expression of caz^KK107486 does not lead to a decrease in the severity of the eye defects resulting from the Scer\GAL4^GMR.PS-driven expression of either Zzzz\UGGAA^W.Scer\UAS or Zzzz\UGGAA^S.Scer\UAS, alone or in combination with Hsap\TARDBP^Scer\UAS.cIb (i.e. reduced size, loss of pigmentation, defective morphology and ommatidial abnormalities).

(Ishiguro et al., 2017)

Overexpression of BacA\p35^Scer\UAS.cHa rescues the fusion of retinal cone cells induced by caz^KK107486 expression.

Co-expression of BacA\p35^Scer\UAS.cHa results in partial suppression of the severe rough eye phenotype seen in flies expressing caz^KK107486 under the control of Scer\GAL4^GMR.PS. Apoptotic cells detected in caz knockdown retinae are significantly reduced in flies expressing BacA\p35^Scer\UAS.cHa.

(Shimamura et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v100291

P{KK107486}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

caz^KK107486

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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