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General Information
Symbol
Dmel\ATP6AP2KK107676
Species
D. melanogaster
Name
FlyBase ID
FBal0235087
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of VhaM8.9KK107676 driven by Scer\GAL4GMR.PF (together with UAS-dicer2 to enhance RNAi efficiency) does not lead to external eye morphology phenotypes, but flies show significant defects in phototaxis behavior compared to controls. Mutant ommatidia and photoreceptors show overall intact organization but photoreceptor-surrounding pigment cells are highly vacuolated and there is an accumulation of vesicular structures in photoreceptor cytoplasm. Electroretinograms (ERGs) from Scer\GAL4GMR.PF>VhaM8.9KK107676 eyes reveal synaptic defects, with complete absence of 'on' or 'off' transients and diminished amplitude photoreceptor re- and depolarization in response to light.

Expression of VhaM8.9KK107676 driven by Scer\GAL4elav.PLu (together with UAS-dicer2 to enhance RNAi efficiency) induces lethality at different development (primarily during larval stages) and surviving flies show reduced spontaneous movements and poor climbing ability. The area of synaptic terminals and number of active zones is significantly decreased and there is a significant increase in the number of satellite boutons at the neuromuscular junction in Scer\GAL4elav.PLu>VhaM8.9KK107676 third instar larvae, compared to controls; synapses also contain large multivesicular body-like organelles and T-bars at active zones are perturbed, and axonal transport defects are detected. Scer\GAL4elav.PLu>VhaM8.9KK107676 adult brains show impairments in autophagy.

Flies with expression of VhaM8.9KK107676 driven by Scer\GAL4247 show elevated activity in response to stimulation (such as banging or soft tapping), with repeated jumping, wing flapping and crashing. Male mutants show subtle defects in short-term (1hr) and complete absence of long-term (24hr) memory in a courtship conditioning paradigm (but show normal naive courtship levels).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The planar polarity defects in the eye which are caused by expression of fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev are partially suppressed by co-expression of VhaM8.9KK107676.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ATP6AP2KK107676
CG8444KK107676
VhaM8.9KK107676
Name Synonyms
Secondary FlyBase IDs
    References (6)