FlyBase Allele Report: Dmel\Ras85D[KK108029]

General Information

Symbol

Dmel\Ras85D^KK108029

Species

D. melanogaster

Name

FlyBase ID

FBal0235321

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{KK108029}

Also Known As

UAS-RAS^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108029}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108029

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates colorectal cancer

modeled by Src42A^CA.UAS

(Cordero et al., 2014)

modeled by Src64B^UAS.cCa

(Cordero et al., 2014)

ameliorates intestinal cancer

modeled by Egfr^UAS.cBa

(Nászai et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion rhythm, with Scer\GAL4^phtm.PO

(Selcho et al., 2017)

Phenotype Manifest In

wing, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing vein, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

Detailed Description

Statement

Reference

Expression of Ras85D^KK108029 (driven by Scer\GAL4^Dll-md23) in type II neuroblast lineage MARCM clones has no significant effect on the clone size or the number of progenitor cells it contains in third instar larvae.

(Rives-Quinto et al., 2017)

Expression of Ras85D^KK108029 under the control of Scer\GAL4^phm.PO significantly reduces the circadian rhythmicity of adult emergence after transfer to constant darkness, compared to controls.

(Selcho et al., 2017)

The expression of Ras85D^KK108029 under the control of Scer\GAL4^c564 has no obvious affect on the inflammatory response to Gram-positive bacterial infection or under basal conditions, as compared to controls.

(Kumar et al., 2016)

Expression of Ras85D^KK108029 in the posterior wing compartment, under the control of Scer\GAL4^en-e16E, generates a wing vein differentiation phenotype.

(Hahn et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ras85D^KK108029/Scer\GAL4^Dll-md23 is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Ras85D^KK108029/Scer\GAL4^Dll-md23 is a suppressor | somatic clone of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Ras85D^KK108029/Scer\GAL4^Dll-md23 is a suppressor | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Ras85D^KK108029/Scer\GAL4^Dll-md23 is a suppressor | somatic clone of increased cell number | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Ras85D^KK108029, Scer\GAL4^NP5130 is a suppressor of hyperplasia phenotype of Scer\GAL4^NP5130, Src42A^CA.UAS

(Cordero et al., 2014)

Ras85D^KK108029, Scer\GAL4^NP5130 is a suppressor of hyperplasia phenotype of Scer\GAL4^NP5130, Src64B^UAS.cCa

(Cordero et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^KK108029, Scer\GAL4^en-e16E has wing phenotype, enhanceable by step^RNAi.UAS, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Ras85D^KK108029, Scer\GAL4^en-e16E has wing vein phenotype, enhanceable by step^RNAi.UAS, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Suppressed by

Statement

Reference

Ras85D^KK108029, Scer\GAL4^en-e16E has wing phenotype, suppressible by step^UAS.FL, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Ras85D^KK108029, Scer\GAL4^en-e16E has wing vein phenotype, suppressible by step^UAS.FL, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Suppressor of

Statement

Reference

Ras85D^KK108029/Scer\GAL4^Dll-md23 is a suppressor | somatic clone of larval brain | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Ras85D^KK108029, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Src64B^UAS.cCa

(Cordero et al., 2014)

Ras85D^KK108029, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Src42A^CA.UAS

(Cordero et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrowth and increased number of progenitor cells characteristic for cno^R2;scrib¹ clones is significantly suppressed by Scer\GAL4^Dll-md23-driven expression of Ras85D^KK108029 in the mutant clones.

(Rives-Quinto et al., 2017)

Knockdown of Ras85D in a step^{dsRNA.Scer\UAS} background, through expression of Ras85D^KK108029 in the posterior wing compartment (both transgenes under the control of Scer\GAL4^en-e16E) generates a more severe Ras85D phenotype than in a wild-type background. The lateral vein L4 is entirely lost, both cross veins are not differentiated and the distal end of the lateral vein L5 is missing.

Expression of step^Scer\UAS.FL suppresses the wing vein defects caused by expression of Ras85D^KK108029 in the posterior compartment of the wing, under the control of Scer\GAL4^en-e16E.

(Hahn et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106642

P{KK108029}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ras85D^KK108029

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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