FlyBase Allele Report: Dmel\TBPH[KK108354]

General Information

Symbol

Dmel\TBPH^KK108354

Species

D. melanogaster

Name

FlyBase ID

FBal0235516

Feature type

allele

Associated gene

Dmel\TBPH

Associated Insertion(s)

Carried in Construct

P{KK108354}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108354}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108354

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 10

CEA

(Jantrapirom et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^50.UAS.EGFP

(Lee et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult CCAP neuron, with Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

adult cuticle, with Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2013)

eye, with Scer\GAL4^GMR.PS

(Jantrapirom et al., 2018)

peptidergic neuron, with Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

ptilinum, with Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2013)

scutellar bristle, with Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2013)

thorax | dorsal, with Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2013)

wing, with Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

wing, with Scer\GAL4^elav-C155

(Vanden Broeck et al., 2013)

wing, with Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2013)

wing, with Scer\GAL4^Toll-6-D42

(Vanden Broeck et al., 2013)

Detailed Description

Statement

Reference

Depending on the driver line insertion, the expression of TBPH^KK108354 under the control of Scer\GAL4^GMR.PS results in rough eye morphology.

(Jantrapirom et al., 2018)

Expression of TBPH^KK108354 driven by Scer\GAL4^CCAP.PP leads to a significant decrease in the number of ccap/bursicon neurons in the ventral nerve cord, compared to controls.

(Vanden Broeck et al., 2015)

Expression of TBPH^KK108354 in the nervous system under the control of Scer\GAL4^nSyb.PU results in immature-looking escapers with unexpanded wings, soft cuticle, unretracted ptilinum, a dimpled dorsal thorax and misoriented scutellar bristles. Wing phenotypes are also seen with Scer\GAL4^elav-C155 and when TBPH^KK108354 is expressed in the motor neurons under the control of Scer\GAL4^D42. A mild wing inflation phenotype is seen when TBPH^KK108354 is expressed in CCAP/bursicon neurons under the control of Scer\GAL4^CCAP.PP.

Expression of TBPH^KK108354 under the control of Scer\GAL4^CCAP.PP has no effect on the morphology or number of CCAP neurons in third instar larvae or pupae up to 60 hours after puparium formation (APF). Outgrowth of adult neurites is normal at 60 hours APF. However, at 72h APF, and in newly eclosed adults, the number of CCAP/bursicon neurons is reduced.

(Vanden Broeck et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^CCAP.PP, TBPH^KK108354 has decreased cell number phenotype, suppressible by Hsap\TARDBP^UAS.cVBa, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has decreased cell number phenotype, suppressible by Hsap\TARDBP^{D169G.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has abnormal neuroanatomy | adult stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has visible phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has abnormal neuroanatomy | adult stage phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^CCAP.PP, TBPH^KK108354 has decreased cell number phenotype, non-suppressible by Hsap\TARDBP^{A90V.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has decreased cell number phenotype, non-suppressible by Hsap\TARDBP^{A315T.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has decreased cell number phenotype, non-suppressible by Hsap\TARDBP^{G287S.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^CCAP.PP, TBPH^KK108354 has adult CCAP neuron phenotype, suppressible by Hsap\TARDBP^{D169G.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has adult CCAP neuron phenotype, suppressible by Hsap\TARDBP^UAS.cVBa, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has peptidergic neuron phenotype, suppressible by Map205^GD8458, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has peptidergic neuron phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has wing phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^CCAP.PP, TBPH^KK108354 has adult CCAP neuron phenotype, non-suppressible by Hsap\TARDBP^{A90V.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has adult CCAP neuron phenotype, non-suppressible by Hsap\TARDBP^{A315T.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Scer\GAL4^CCAP.PP, TBPH^KK108354 has adult CCAP neuron phenotype, non-suppressible by Hsap\TARDBP^{G287S.UAS.cVBa}, Scer\GAL4^CCAP.PP

(Vanden Broeck et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Diap1^Scer\UAS.cHa suppresses the reduction in CCAP/bursicon neuron number seen in newly eclosed flies expressing TBPH^KK108354 under the control of Scer\GAL4^CCAP.PP.

Expression of Map205^GD8458 suppresses the immature wing inflation phenotype seen when TBPH^KK108354 is expressed under the control of Scer\GAL4^nSyb.PU.

Expression of Map205^GD8458 suppresses the wing inflation phenotype and loss of CCAP/bursicon neurons seen when TBPH^KK108354 is expressed under the control of Scer\GAL4^CCAP.PP.

(Vanden Broeck et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\TARDBP^{D169G.Scer\UAS.cVBa} or Hsap\TARDBP^{Scer\UAS.cVBa} (but not Hsap\TARDBP^{A90V.Scer\UAS.cVBa}, Hsap\TARDBP^{A315T.Scer\UAS.cVBa} or Hsap\TARDBP^{G287S.Scer\UAS.cVBa}) suppresses the loss of bursicon neurons seen in animals with expression of TBPH^KK108354 driven by Scer\GAL4^nSyb.PU.

(Vanden Broeck et al., 2015)

Expression of BacA\p35^Scer\UAS.cHa suppresses the reduction in CCAP/bursicon neuron number seen in newly eclosed flies expressing TBPH^KK108354 under the control of Scer\GAL4^CCAP.PP. The wing inflation defects are also rescued.

(Vanden Broeck et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v104401

P{KK108354}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

TBPH^KK108354

TBPH^RNAi

(Vanden Broeck et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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