FB2025_01 , released February 20, 2025
Allele: Dmel\CCT4KK108551
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General Information
Symbol
Dmel\CCT4KK108551
Species
D. melanogaster
Name
FlyBase ID
FBal0235643
Feature type
allele
Associated gene
Dmel\CCT4
Associated Insertion(s)
Carried in Construct
P{KK108551}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK108551}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK108551
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  intrinsic cardiomyopathy
      CEA
      (Melkani et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing CCT4KK108551 under the control of Scer\GAL4ey.PU results in complete loss of eye disc field (with no effect on antennal field) in third instar larvae which, in turn, leads to the complete absence of head structures (but normal thorax and abdomen) in pharate adults.

      Expressing CCT4KK108551 under the control of Scer\GAL4en.PU results in reduced cell proliferation and an almost complete loss of the wing disc posterior compartment, while expression under the control of either Scer\GAL4Bx-MS1096 or Scer\GAL4nub.PU results in severely malformed wings.

      Expressing CCT4KK108551 under the control of Scer\GAL4ptc.PU leads to increased cell death in the wing pouch, a narrower anterior-posterior compartment boundary of the wing disc and greatly reduced salivary glands in third instar larvae compared to controls. In adults, the number and size of cells in the wing blade domain between L3 and L4 veins and the overall domain size are significantly reduced compared to wild-type controls.

      Expressing CCT4KK108551 under the control of Scer\GAL4GMR.PU fails to induce cell death in the posterior compartment of the eye disc in third instar larvae, and results in adults with normal eyes.

      (Kim and Choi, 2019)

      The expression of CCT4KK108551 under the control of either Scer\GAL4tin.CΔ4 or Scer\GAL4HCH.Hand leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to near or fully penetrant cardiac defects, respectively, namely morphological defects, non-beating heart segments and non-beating hearts, as compared to controls.

      The expression of CCT4KK108551 under the control of Scer\GAL4tin.CΔ4 also leads to progressive cardiac dysfunction, as shown by significant increases in heart period, arrhythmia, and diastolic and systolic intervals, and by significant decreases in fractional shortening, and diastolic and systolic diameter, as compared to controls. These hearts show a severe and progressive disorganization in the actin- and myosin-containing myofibrils, as compared to controls.

      (Melkani et al., 2017)

      The expression of CCT4KK108551 under the control of Scer\GAL4elav-C155 leads to the neuromuscular junctions of third instar larval muscles 6/7 exhibiting clustering and decreased number of terminal boutons, as compared to controls.

      (Raut et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      CCT4KK108551, Scer\GAL4ptc.PU has wing blade phenotype, enhanceable by Rheb2D1/Rheb[+]

      (Kim and Choi, 2019)
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      NOT Enhancer of
      Statement
      Reference

      CCT4KK108551, Scer\GAL4ptc.PU is a non-enhancer of wing blade phenotype of Scer\GAL4ptc.PU, btl::Egfrλ.UAS

      (Kim and Choi, 2019)
      Suppressor of
      Statement
      Reference

      CCT4KK108551, Scer\GAL4ptc.PU is a suppressor | partially of wing blade phenotype of CycEUAS.cRa, Scer\GAL4ptc.PU

      (Kim and Choi, 2019)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      CCT4KK108551 is rescued by Scer\GAL4ptc.PU/CCT4UAS.cKa

      (Kim and Choi, 2019)

      CCT4KK108551 is rescued by Scer\GAL4ey.PU/CCT4UAS.cKa

      (Kim and Choi, 2019)

      CCT4KK108551 is rescued by Scer\GAL4nub.PU/CCT4UAS.cKa

      (Kim and Choi, 2019)
      Comments

      Co-expressing CCT4UAS.cKa with CCT4KK108551 under the control of Scer\GAL4ptc.PU rescues the size reduction of the wing blade domain between L3 and L4 veins, while co-expression under the control of Scer\GAL4nub.PU rescues wing formation, and co-expression under the control of Scer\GAL4ey.PU fully rescues head formation defects induced by CCT4KK108551 expression alone.

      (Kim and Choi, 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CCT4KK108551
      CG5525KK108551
      Name Synonyms
      Secondary FlyBase IDs
        References (8)