FlyBase Allele Report: Dmel\upd3[KK110348]

General Information

Symbol

Dmel\upd3^KK110348

Species

D. melanogaster

Name

FlyBase ID

FBal0236805

Feature type

allele

Associated gene

Dmel\upd3

Associated Insertion(s)

Carried in Construct

P{KK110348}

Also Known As

UAS-upd3^RNAi, UAS-upd3-IR, upd3 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK110348}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK110348

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates hematologic cancer

modeled by mxc¹

(Kinoshita et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

In a tuberculosis model by abdominal injection with Mycobacterium marinum, adult flies expressing upd3^KK110348 under the control of Scer\GAL4^crq.Unk present a significant increase in survival, and a significant decrease in the bacterial burden, as compared to wild-type control flies.

(Péan et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^crq.Unk

(Péan et al., 2017)

abnormal size, with Scer\GAL4^en-e16E

(Djiane et al., 2013)

Phenotype Manifest In

wing | posterior, with Scer\GAL4^en-e16E

(Djiane et al., 2013)

Detailed Description

Statement

Reference

The expression of upd3^KK110348 under the control of Scer\GAL4^crq.Unk leads to a significant increase in survival, and a significant decrease in the bacterial burden in adults infected with Mycobacterium marinum, as compared to wild-type controls; non-infected individuals present similar survival to wild-type controls.

The expression of upd3^KK110348 under the control of Scer\GAL4^Hml.Δ has no significant effect in the internalization of bacteria by hemocytes in adults infected with Mycobacterium marinum, as compared to wild-type controls.

(Péan et al., 2017)

Expression of upd3^KK110348 RNAi under the control Scer\GAL4^Hml.Δ (but not that of either the Scer\GAL4^how-24B or the Scer\GAL4^mex1.PU drivers) significantly improves the lowered survival of adult flies on lard-supplemented diet compared to controls. upd3^KK110348 expression driven by Scer\GAL4^Hml.Δ does not alter plasmatocyte count in aged adult flies kept compared to controls.

(Woodcock et al., 2015)

Scer\GAL4^en-e16E-mediated expression results in a strong reduction in size of the posterior compartment of the adult wing.

(Djiane et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

upd3^KK110348, Scer\GAL4^NP0001 is a suppressor | partially of increased occurrence of cell division | adult stage phenotype of Sams^GD1486, Scer\GAL4^NP0001

(Obata et al., 2018)

upd3^KK110348/Scer\GAL4^NP0001 is a suppressor of increased cell number | adult stage phenotype of Tnks⁵⁰³/Tnks¹⁹

(Wang et al., 2016)

upd3^KK110348/Scer\GAL4^NP0001 is a suppressor of increased occurrence of cell division | adult stage phenotype of Tnks⁵⁰³/Tnks¹⁹

(Wang et al., 2016)

upd3^KK110348, Scer\GAL4^ptc-559.1 is a suppressor | partially of hyperplasia phenotype of N^UAS.icd, Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

upd3^KK110348, Scer\GAL4^NP0001 is a suppressor | partially of adult midgut phenotype of Sams^GD1486, Scer\GAL4^NP0001

(Obata et al., 2018)

upd3^KK110348/Scer\GAL4^NP0001 is a suppressor of adult midgut phenotype of Tnks⁵⁰³/Tnks¹⁹

(Wang et al., 2016)

upd3^KK110348/Scer\GAL4^NP0001 is a suppressor of intestinal stem cell | adult stage phenotype of Tnks⁵⁰³/Tnks¹⁹

(Wang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of proliferating (EdU+) cell in the posterior adult midgut epithelium characteristic for flies expressing Scer\GAL4^{Myo31DF-NP0001} under the control of Sam-S^GD1486 (combined with tub-Gal80[ts] for added temporal control) is partially suppressed by co-expression of upd3^KK110348.

(Obata et al., 2018)

The increase in the numbers of intestinal stem cells and mitotic cells present in Tnks¹⁹/Tnks⁵⁰³ transheterozygous mutant adult midguts is suppressed by expressing upd3^KK110348 under the control of Scer\GAL4^{Myo31DF-NP0001}.

(Wang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106869

P{KK110348}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

upd3^KK110348

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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