Expression of Inx7^KK112684 under the control of Scer\GAL4^ey-OK107 in adults does not significantly affect 3 hour aversive olfactory memory or the residual anaesthesia-resistant memory (ARM) after cold-shock, compared to controls; expression under the control of Scer\GAL4^VT000765 also does not affect ARM, compared to controls.

Expression of Inx7^KK112684 under the control of Scer\GAL4^17d, the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R13F02, the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02, or the split-GAL4 pair of Scer\GAL4^DBD.R14C08 and Hsap\RELA^AD.R12C11, does not affect visual short term memory following training with pain stimulus in adult flies, compared to controls.

Expression of Inx7^KK112684 under the control of Scer\GAL4^tj.PU (along with expression of UAS-Dcr2 to increase RNAi efficiency) does not cause sterility.

Expression of inx7^KK112684 in both the anterior paired lateral (APL) and dorsal paired medial (DPM) neurons in the mushroom body (using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously) results in impaired 3 hour (intermediate-term) memory after associative conditioning compared to controls, although initial learning is normal in these flies. The anesthesia-resistant memory (ARM) component of intermediate-term memory is normal in these flies.

Impairment of 3 hour memory after associative conditioning is still seen in flies in which expression of inx7^KK112684 using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously is limited to the adult stage by using the temperature sensitive Scer\GAL80^ts.αTub84B allele to restrict expression before this stage.

The gross anatomy of the APL and DPM neurons in flies expressing inx7^KK112684 using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously is not different from that in control flies.

Flies expressing inx7^KK112684 in the DPM neurons under the control of Scer\GAL4⁵⁰¹⁵ have normal 3 hour memory after associative conditioning.

Flies expressing inx7^KK112684 under the control of Scer\GAL4^GH146 have impaired 3 hour memory after associative conditioning compared to controls. If the expression is refined by adding the Scer\GAL80^Cha.PK repressor (this reduces expression in the APL neurons to undetectable levels, leaving expression in only a subset of projection neurons), the flies have normal 3 hour memory.