FlyBase Allele Report: Dmel\Zasp66[KK112973]

General Information

Symbol

Dmel\Zasp66^KK112973

Species

D. melanogaster

Name

FlyBase ID

FBal0238511

Feature type

allele

Associated gene

Dmel\Zasp66

Associated Insertion(s)

Carried in Construct

P{KK112973}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK112973}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK112973

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

majority die during pupal stage, with Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

partially lethal, with Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

Phenotype Manifest In

indirect flight muscle cell, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

M band, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

myofibril, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

Z disc, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

Z disc, with Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

Detailed Description

Statement

Reference

Expression of Zasp66^KK112973 under the control of Scer\GAL4^Mef2.PR results in 83% pupal lethality and surviving adults can fly. Mild defects in Z-disc structure are seen in the indirect flight muscles (IFMs) of adult flies.

Expression of Zasp66^KK112973 under the control of Scer\GAL4^Mef2.PR (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi) results in 98% pupal lethality and adults escapers are flightless. Mild myofibrillar abnormalities are seen in the IFMs of most adult escapers and distorted M-lines and Z-discs are occasionally seen. In more severe cases, sarcomere integrity is lost and M-lines and Z-discs are severely disrupted. At 48 hours after puparium formation the IFM myofibrils are thinner than normal and frayed and Z-discs are irregular.

Adults expressing Zasp66^KK112973 under the control of Scer\GAL4^Act88F.PB are able to fly and the indirect flight muscles show no severe defects ultrastructurally.

(Katzemich et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Mef2.PR, Zasp66^KK112973 has majority die during pupal stage phenotype, enhanceable by Zasp52[+]/Zasp52^unspecified

(Katzemich et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of H zone phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of indirect flight muscle cell phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of Z disc phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Other

Statement

Reference

Scer\GAL4^Act88F.PB, Zasp52^KK101276, Zasp66^KK112973 has myofibril phenotype

(Katzemich et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Zasp52^unspecified/+ enhances the pupal lethality caused by expression of Zasp66^KK112973 under the control of Scer\GAL4^Mef2.PR.

Adults co-expressing Zasp52^KK101276 and Zasp66^KK112973 under the control of Scer\GAL4^Act88F.PB have severe defects in the indirect flight muscles. Myofibrils are often frayed and unstable, with severely distorted Z-discs and H-zones.

(Katzemich et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v102980

P{KK112973}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Zasp66^KK112973

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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