Nearly all RecQ414 homozygous mutants survive for up to 8 days under normal culture conditions. However they exhibit developmental delays when compared with heterozygous siblings or wild type controls and eventually die at the early pupal stage.
Oocytes from females bearing RecQ414 germ-line clones fail to develop beyond stage 6 of oogenesis.
At 5 days AED, the amount of DNA normalized by cell number is much lower in RecQ414 salivary glands, compared to wild type. Mutant salivary glands also have abnormally small cells and nuclei.
At 5 days AED, RecQ414 mutant brains incorporate much less BrdU than the wild type, indicating impaired DNA replication.
Metaphase spreads from RecQ414 larval brains show a much higher frequency of aberrant patterns (failure of sister chromatid association and broken chromosomes) compared to wild type.
RecQ414 show sensitivity to the mutagens paraquat and gamma irradiation, as judged by survival rates compared to controls.
Compared with wild type, more cells from RecQ414 mutant wing imaginal discs accumulate at G2/M phase at the expense of G1/G0 and S phase cells.
RecQ414 clones in the wing disc have fewer cells and are therefore smaller than their wild type twin spots.
RecQ414, RecQ4+t8.6 flies in which the RecQ4+t8.6 rescuing transgene is specifically deleted in the eye by using Scer\FLP1ey.PN have small rough eyes with disorganized and fewer ommatidia.