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General Information
Symbol
Dmel\RecQ414
Species
D. melanogaster
Name
FlyBase ID
FBal0239075
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Inserted_sequence:

CCTAGGGTCGACCCGCG

Comment:

The start codon was replaced with CCTAGGGTCGACCCGCG, causing a frameshift. The endogenous copy of RecQ4 was replaced with the mutated copy by targeted recombination.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

RecQ4 gene in which the start codon ATG is replaced with CCTAGGGTCGACCCGCG, thus disrupting the translation start site and shifting the open reading frame.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nearly all RecQ414 homozygous mutants survive for up to 8 days under normal culture conditions. However they exhibit developmental delays when compared with heterozygous siblings or wild type controls and eventually die at the early pupal stage.

Oocytes from females bearing RecQ414 germ-line clones fail to develop beyond stage 6 of oogenesis.

At 5 days AED, the amount of DNA normalized by cell number is much lower in RecQ414 salivary glands, compared to wild type. Mutant salivary glands also have abnormally small cells and nuclei.

At 5 days AED, RecQ414 mutant brains incorporate much less BrdU than the wild type, indicating impaired DNA replication.

Metaphase spreads from RecQ414 larval brains show a much higher frequency of aberrant patterns (failure of sister chromatid association and broken chromosomes) compared to wild type.

RecQ414 show sensitivity to the mutagens paraquat and gamma irradiation, as judged by survival rates compared to controls.

Compared with wild type, more cells from RecQ414 mutant wing imaginal discs accumulate at G2/M phase at the expense of G1/G0 and S phase cells.

RecQ414 clones in the wing disc have fewer cells and are therefore smaller than their wild type twin spots.

RecQ414, RecQ4+t8.6 flies in which the RecQ4+t8.6 rescuing transgene is specifically deleted in the eye by using Scer\FLP1ey.PN have small rough eyes with disorganized and fewer ommatidia.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

When the in vivo DSB repair construct, P{Act-RFP.Rr3}, is cut (i.e. in flies with genotype Disc\RFPRr3.Scer\SceI.RS.Act5C, Scer\SCEIUbi-p63E.PP), RecQ414 homozygotes show a significantly reduced survival ratio compared with heterozygous animals.

Complementation and Rescue Data
Partially rescued by
Comments

RecQ4+t8.6 fully rescues the lethal phenotype of RecQ414 mutants.

Expression of RecQ4Scer\UAS.cXa using Scer\GAL4Act fully rescues the lethal phenotype of RecQ414 mutants.

Expression of RecQ4FL.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4Act fully rescues the lethality, BrdU incorporation and gamma-irradiation sensitivity phenotypes of RecQ414 mutants.

Expression of RecQ4Δ868-1579.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4Act does not rescue the lethality or BrdU incorporation phenotypes of RecQ414 mutants, but it does fully rescue the gamma-irradiation sensitivity phenotype.

Expression of RecQ4Δ1234-1579.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4Act partially rescues (at approximately 10% efficiency) the lethality of RecQ414 mutants, and fully rescues the BrdU incorporation and gamma-irradiation sensitivity phenotypes.

Expression of RecQ4Δ1-807.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4Act fails to rescue the lethality, BrdU incorporation or gamma-irradiation sensitivity phenotypes of RecQ414 mutants.

Expression of RecQ4Δ868-1207.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4Act fails to rescue the lethality or BrdU incorporation phenotypes of RecQ414 mutants, but this does rescue the gamma-irradiation sensitivity phenotype.

Expression of RecQ4Δ1-1207.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4Act fails to rescue the lethality, BrdU incorporation or gamma-irradiation sensitivity phenotypes of RecQ414 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RecQ414
dRecQ414
Name Synonyms
Secondary FlyBase IDs
    References (1)