Allele Dmel\RecQ4¹⁴

General Information
Symbol	Dmel\RecQ4¹⁴	Species	D. melanogaster
Name		FlyBase ID	FBal0239075
Feature type	allele	Associated gene	Dmel\RecQ4

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	I-CreI endonuclease, ends-in gene targeting
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Xu et al., 2009)
Mutagen	ends-in gene targeting (Xu et al., 2009) I-CreI endonuclease (Xu et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References complex substitution 3L:8,168,955..8,168,956 comment=The start codon was replaced with CCTAGGGTCGACCCGCG, causing a frameshift. The endogenous copy of RecQ4 was replaced with the mutated copy by targeted recombination. (Xu et al., 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Dp(3;3)RecQ4^+.14 (Xu et al., 2009)
Nature of the lesion	Statement Reference RecQ4[14] is the final product of the targeted 'ends-in' recombination of the RecQ4 gene, in which P{FRT(RecQ4[TARG.Scer\SceI.RS].Crei\I-CreI.RS.w[+])} was used as the donor template and Dp(3;3)RecQ4[+.14] was generated as an intermediate. Flies bearing RecQ4[14] have a single copy of the RecQ4 gene in which the start codon ATG is replaced with CCTAGGGTCGACCCGCG, thus disrupting the transation start site and shifting the open reading frame. (Xu et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	chemical sensitive \| recessive (Xu et al., 2009) decreased cell number \| somatic clone (Xu et al., 2009) decreased cell size \| recessive (Xu et al., 2009) developmental rate defective \| recessive (Xu et al., 2009) DNA repair defective \| recessive (Xu et al., 2009) endomitotic cell cycle defective \| recessive (Xu et al., 2009) female sterile \| germline clone (Xu et al., 2009) lethal \| pupal stage \| recessive (Xu et al., 2009) mitotic cell cycle defective \| recessive (Xu et al., 2009) radiation sensitive \| recessive (Xu et al., 2009)
Phenotype Manifest In
	cell & dorsal mesothoracic disc (Xu et al., 2009) cell & larval brain (Xu et al., 2009) cell & larval salivary gland (Xu et al., 2009) chromosome (Xu et al., 2009) eye \| somatic clone (Xu et al., 2009) ommatidium \| somatic clone (Xu et al., 2009) oocyte \| germline clone (Xu et al., 2009)
Detailed Description
	Statement Reference Nearly all RecQ4[14] homozygous mutants survive for up to 8 days under normal culture conditions. However they exhibit developmental delays when compared with heterozygous siblings or wild type controls and eventually die at the early pupal stage. Oocytes from females bearing RecQ4[14] germ-line clones fail to develop beyond stage 6 of oogenesis. At 5 days AED, the amount of DNA normalized by cell number is much lower in RecQ4[14] salivary glands, compared to wild type. Mutant salivary glands also have abnormally small cells and nuclei. At 5 days AED, RecQ4[14] mutant brains incorporate much less BrdU than the wild type, indicating impaired DNA replication. Metaphase spreads from RecQ4[14] larval brains show a much higher frequency of aberrant patterns (failure of sister chromatid association and broken chromosomes) compared to wild type. RecQ4[14] show sensitivity to the mutagens paraquat and gamma irradiation, as judged by survival rates compared to controls. Compared with wild type, more cells from RecQ4[14] mutant wing imaginal discs accumulate at G2/M phase at the expense of G1/G0 and S phase cells. RecQ4[14] clones in the wing disc have fewer cells and are therefore smaller than their wild type twin spots. RecQ4[14], RecQ4[+t8.6] flies in which the RecQ4[+t8.6] rescuing transgene is specifically deleted in the eye by using Scer\FLP1[ey.PN] have small rough eyes with disorganized and fewer ommatidia. (Xu et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Disc\RFP^{Rr3.Scer\SceI.RS.Act5C}, RecQ4¹⁴, Scer\SCEI^Ubi-p63E.PP has lethal \| recessive phenotype (Xu et al., 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference When the in vivo DSB repair construct, P{Act-RFP.Rr3}, is cut (i.e. in flies with genotype Disc\RFP[Rr3.Scer\SceI.RS.Act5C], Scer\SCEI[Ubi-p63E.PP]), RecQ4[14] homozygotes show a significantly reduced survival ratio compared with heterozygous animals. (Xu et al., 2009)
Complementation & Rescue Data
Rescued by	RecQ4¹⁴ is rescued by RecQ4^+t8.6 (Xu et al., 2009) RecQ4¹⁴ is rescued by RecQ4^Scer\UAS.cXa/Scer\GAL4^Act.PU (Xu et al., 2009) RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{FL.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009) RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1207.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009) RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1579.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009) RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1234-1579.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009)
Partially rescued by	RecQ4¹⁴ is partially rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1234-1579.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009)
Not rescued by	RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1-807.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009) RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1-1207.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009) RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1207.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009) RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1579.Scer\UAS.T:Zzzz\FLAG} (Xu et al., 2009)
Comments	RecQ4[+t8.6] fully rescues the lethal phenotype of RecQ4[14] mutants. Expression of RecQ4[Scer\UAS.cXa] using Scer\GAL4[Act] fully rescues the lethal phenotype of RecQ4[14] mutants. Expression of RecQ4[FL.Scer\UAS.T:Zzzz\FLAG] using Scer\GAL4[Act] fully rescues the lethality, BrdU incorporation and gamma-irradiation sensitivity phenotypes of RecQ4[14] mutants. Expression of RecQ4[Δ868-1579.Scer\UAS.T:Zzzz\FLAG] using Scer\GAL4[Act] does not rescue the lethality or BrdU incorporation phenotypes of RecQ4[14] mutants, but it does fully rescue the gamma-irradiation sensitivity phenotype. Expression of RecQ4[Δ1234-1579.Scer\UAS.T:Zzzz\FLAG] using Scer\GAL4[Act] partially rescues (at approximately 10% efficiency) the lethality of RecQ4[14] mutants, and fully rescues the BrdU incorporation and gamma-irradiation sensitivity phenotypes. Expression of RecQ4[Δ1-807.Scer\UAS.T:Zzzz\FLAG] using Scer\GAL4[Act] fails to rescue the lethality, BrdU incorporation or gamma-irradiation sensitivity phenotypes of RecQ4[14] mutants. Expression of RecQ4[Δ868-1207.Scer\UAS.T:Zzzz\FLAG] using Scer\GAL4[Act] fails to rescue the lethality or BrdU incorporation phenotypes of RecQ4[14] mutants, but this does rescue the gamma-irradiation sensitivity phenotype. Expression of RecQ4[Δ1-1207.Scer\UAS.T:Zzzz\FLAG] using Scer\GAL4[Act] fails to rescue the lethality, BrdU incorporation or gamma-irradiation sensitivity phenotypes of RecQ4[14] mutants. (Xu et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	dRecQ4¹⁴ (Xu et al., 2009) RecQ4¹⁴
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Xu et al., 2009, PLoS ONE 4(7): e6107 dRecQ4 is required for DNA synthesis and essential for cell proliferation in Drosophila. [FBrf0208182]

Allele Dmel\RecQ414

Allele Dmel\RecQ4¹⁴