Allele Dmel\sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0239091
Feature type	allele	Associated gene	Dmel\sn

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Zanet et al., 2009) in vitro construct - regulatory fusion (Zanet et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS and P\T regulatory sequences drive expression of a full-length sn cDNA (RH62992) with T:Avic\GFP[EGFP] fused to the N-terminus. (Zanet et al., 2009)
Carried in construct	P{UASp-sn.EGFP} (Zanet et al., 2009, Zanet et al., 2012)
Tagged with	T:Avic\GFP^EGFP (Zanet et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}/Scer\GAL4^hs.PB rescues sn³ (Zanet et al., 2009) sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}/Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues sn²⁸ (Zanet et al., 2009, Zanet et al., 2012) sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}/Scer\GAL4^sn.PZ rescues Df(1)C128 (Zanet et al., 2012) sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}/Scer\GAL4^sn.PZ rescues sn²⁸ (Zanet et al., 2012) sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}/Scer\GAL4^srp.Hemo rescues Df(1)C128 (Zanet et al., 2009)
Partially rescues	sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}/Scer\GAL4^srp.Hemo partially rescues sn^X2 (Zanet et al., 2009)
Comments	Expression of sn[Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[sn.PZ] rescues the macrophage migration defects seen in sn[28] mutant stage 15 embryos. The reduction in the number of actin rich filopodia and microspikes within the lamellae of sn[28] mutant macrophages is also rescued. Expression of sn[Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] rescues the female sterility and actin bundle formation defects seen in sn[28] mutant nurse cells. Expression of sn[Scer\UAS.P\T.T:Avic\GFP-EGFP] under the control of Scer\GAL4[sn.PZ] rescues the macrophage migration defects seen in Df(1)C128 mutant stage 15 embryos. (Zanet et al., 2012) Scer\GAL4[srp.Hemo]-driven expression of sn[Scer\UAS.P\T.T:Avic\GFP-EGFP] restores the polarity and the dynamic leading edge cortex that is normally lacking in plasmatocytes in Df(1)C128 embryos. Normal developmental migration of plasmatocytes is also restored. Scer\GAL4[srp.Hemo]-driven expression of sn[Scer\UAS.P\T.T:Avic\GFP-EGFP] provides significant rescue of the reduced plasmatocyte recruitment seen in sn[X2] embryos after laser-induced wounding. Scer\GAL4[nos.UTR.T:Hsim\VP16]-driven expression of sn[Scer\UAS.P\T.T:Avic\GFP-EGFP] fully restores the formation of actin bundles around nurse cell nuclei that is normally lacking in sn[28] mutants. Female ferility is also restored. Scer\GAL4[hs.PB]-driven expression of sn[Scer\UAS.P\T.T:Avic\GFP-EGFP] fully rescues the gnarled bristle phenotype exhibited by sn[3] mutants. (Zanet et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Zanet et al., 2012, J. Cell Biol. 197(4): 477--486 Fascin promotes filopodia formation independent of its role in actin bundling. [FBrf0218285] Zanet et al., 2009, Development 136(15): 2557--2565 Fascin is required for blood cell migration during Drosophila embryogenesis. [FBrf0208475]

Allele Dmel\snScer\UAS.P\T.T:Avic\GFP-EGFP

Allele Dmel\sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP}