FlyBase Allele Report: Dmel\Pink1[UAS.cYa]

General Information

Symbol

Dmel\Pink1^UAS.cYa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Yang

FlyBase ID

FBal0239191

Feature type

allele

Associated gene

Dmel\Pink1

Associated Insertion(s)

Carried in Construct

P{UAS-Pink1.Y}

Also Known As

UAS-dPink1, UAS-PINK1

Key Links

Transgenic product class

wild_type

(Yang et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yang et al., 2006)

Mutagen

in vitro construct

(Yang et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Pink1.Y}

Cytology

Description

UASt regulatory sequences drive expression of Pink1 coding sequences.

(Yang et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pink1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^{P525L.UAS.RFP(Unk),Tag:HA}

(Chen et al., 2016)

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cFa

(Krzystek et al., 2021)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^{80.UAS.Tag:FLAG}

(Li et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval motor neuron | larval stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

mitochondrion & dorso-lateral dopaminergic neuron, with Scer\GAL4^ple.PF

(Yang et al., 2008)

mitochondrion | larval stage, with Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

mitochondrion | larval stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

Detailed Description

Statement

Reference

Ectopic expression of Pink1^Scer\UAS.cYa under the control of Scer\GAL4^ple.PF has no significant effect on the number of dopaminergic PPL1 neurons in the adult brain.

(Gehrke et al., 2015)

Expression of Pink1^Scer\UAS.cYa under the control of Scer\GAL4^ple.PF in larval motor neurons results in decreased axonal mitochondrial transport flux and velocity in both anterograde and retrograde directions, decreased mitochondrial length, and decreased accumulation of mitochondria in the most distal boutons of neuromuscular junctions, as compared with controls.

(Liu et al., 2012)

Mitochondrial aggregates form in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1^Scer\UAS.cYa under the control of Scer\GAL4^ple.PF. The nonaggregated mitochondria appear spherical and tubular mitochondrial structures are rarely seen.

The fraction of cultured dopaminergic neurons having thread-like tubular mitochondria is lower in cells expressing Pink1^Scer\UAS.cYa under the control of Scer\GAL4^ple.PF than in control cells.

(Yang et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Pink1^UAS.cYa, Scer\GAL4^pnt-14-94 is an enhancer of abnormal neuroanatomy phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^pnt-14-94

(Lee et al., 2013)

Suppressor of

Statement

Reference

Pink1^UAS.cYa, Scer\GAL4^Appl.G1a is a suppressor of increased cell death | larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^ple.PF, Tom20^KK107626

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a suppressor | partially of decreased cell number | adult stage phenotype of Scer\GAL4^ple.PF, Tom20^KK107626

(Gehrke et al., 2015)

NOT Suppressor of

Statement

Reference

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a non-suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^ple.PF, Tom40^JF02030

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^Mhc.PU is a non-suppressor of visible | adult stage | progressive phenotype of Scer\GAL4^Mhc.PU, pum^UAS.cYa

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^Mhc.PU is a non-suppressor of abnormal jumping phenotype of Scer\GAL4^Mhc.PU, pum^UAS.cYa

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^ple.PF, Tim8^JF01533

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a non-suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^ple.PF, Tim8^JF01533

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^ple.PF, Tom40^JF02030

(Gehrke et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Pink1^UAS.cYa, Scer\GAL4^ple.PF has mitochondrion & dorso-lateral dopaminergic neuron phenotype, non-suppressible by Drp1^{Tag:FLAG,Tag:CALI(TC),Tag:HA}

(Yang et al., 2008)

Pink1^UAS.cYa, Scer\GAL4^ple.PF has mitochondrion & dorso-lateral dopaminergic neuron phenotype, non-suppressible by opa1-like[+]/Opa1^EY09863

(Yang et al., 2008)

Pink1^UAS.cYa, Scer\GAL4^ple.PF has mitochondrion & dorso-lateral dopaminergic neuron phenotype, non-suppressible by park^UAS.Tag:HA, Scer\GAL4^ple.PF

(Yang et al., 2008)

Enhancer of

Statement

Reference

Pink1^UAS.cYa, Scer\GAL4^pnt-14-94 is an enhancer of type II neuroblast | increased number phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^pnt-14-94

(Lee et al., 2013)

Suppressor of

Statement

Reference

Pink1^UAS.cYa, Scer\GAL4^Appl.G1a is a suppressor of mitochondrion | larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Pink1^UAS.cYa, Scer\GAL4^Appl.G1a is a suppressor of larval brain | larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a suppressor | partially of dopaminergic PPL1 neuron | adult stage phenotype of Scer\GAL4^ple.PF, Tom20^KK107626

(Gehrke et al., 2015)

NOT Suppressor of

Statement

Reference

Pink1^UAS.cYa, Scer\GAL4^Appl.G1a is a non-suppressor of mitochondrion | larval stage phenotype of Hsap\SNCA^1-120.UAS, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a non-suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Scer\GAL4^ple.PF, Tom40^JF02030

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^Mhc.PU is a non-suppressor of wing | adult stage | progressive phenotype of Scer\GAL4^Mhc.PU, pum^UAS.cYa

(Gehrke et al., 2015)

Pink1^UAS.cYa, Scer\GAL4^ple.PF is a non-suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Scer\GAL4^ple.PF, Tim8^JF01533

(Gehrke et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of dopaminergic (DA) PPL1 neurons along with the mitochondrial aggregations in DA neurons observed in the brains of adult flies expressing Tom20^KK107626 RNAi under the control of Scer\GAL4^ple.PF are partially suppressed by co-expression of Pink1^Scer\UAS.cYa but this does not affect the loss of PPL1 neurons induced by expression of either Tim8^JF01533 or Tom40^JF02030. The wing posture and jumping ability defects characteristic for adult flies expressing pum^Scer\UAS.cYa controlled by the Scer\GAL4^Mhc.PU driver are also not rescued by co-expression of Pink1^Scer\UAS.cYa.

(Gehrke et al., 2015)

Co-expression of Pink1^Scer\UAS.cYa enhances the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^pnt-14-94.

(Lee et al., 2013)

The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1^Scer\UAS.cYa under the control of Scer\GAL4^ple.PF is not modified by Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} or by opa1-like^EY09863/+.

The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1^Scer\UAS.cYa under the control of Scer\GAL4^ple.PF is not suppressed by co-expression of park^{Scer\UAS.T:Ivir\HA1}.

(Yang et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Mhc.PW/Pink1^UAS.cYa rescues Pink1^RNAi.UAS.cYa

(Yang et al., 2006)

Comments

The expression of Pink1^Scer\UAS.cYa, alongside Pink1^{dsRNA.Scer\UAS}, both under the control of Scer\GAL4^Mhc.PW, rescues the tissue-specific RNAi phenotype.

(Yang et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pink1^Scer\UAS.cYa

Pink1^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Yang

Secondary FlyBase IDs

References (12)

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