FlyBase Allele Report: Dmel\msd1[ex51]

General Information

Symbol

Dmel\msd1^ex51

Species

D. melanogaster

Name

FlyBase ID

FBal0239240

Feature type

allele

Associated gene

Dmel\msd1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Wainman et al., 2009)

Progenitor genotype

msd1^EY11673

(Wainman et al., 2009)

Cytology

Description

Remobilization of P{EPgy2}msd1^EY11673 results in a deletion internal to the P-element.

(Wainman et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Wainman et al., 2009)

abnormal mitotic cell cycle

(Wainman et al., 2009)

female sterile

(Wainman et al., 2009)

female sterile (with Df(3L)bab-PG)

(Wainman et al., 2009)

viable

(Wainman et al., 2009)

viable (with Df(3L)bab-PG)

(Wainman et al., 2009)

Phenotype Manifest In

spindle

(Wainman et al., 2009)

Detailed Description

Statement

Reference

Female meiotic spindles in msd1^ex51 mutant embryos appear to show qualitative differences to wild-type, both in terms of apparent spindle density and/or length, and in chromosome alignment. However, bipolar spindle organization is not affected, and many msd1^ex51 mutant embryos undergo a variable number of mitotic divisions, before accumulating defects and arresting prior to cellularization.

The primary defect observed in msd1^ex51 mutant embryos is an increase in mitotic spindle length during metaphase, and a reduction in mitotic spindle density, when compared with wild-type spindles.

msd1^ex51 mutant third-instar larvae exhibit an increased mitotic index and a decrease in the proportion of cells in anaphase, suggesting that reduction of msd1 levels results in a prolonged prometaphase/metaphase.

msd1^ex51/Df(3L)bab-PG hemizygous female flies are viable but sterile. Mutant embryos laid by hemizygous mothers arrest development at an earlier stage than those laid by homozygous msd1^ex51 mothers.

(Wainman et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

msd1^ex51 has abnormal mitotic cell cycle phenotype, enhanceable by cnn^HK21

(Wainman et al., 2009)

Enhancer of

Statement

Reference

msd1^ex51 is an enhancer of abnormal mitotic cell cycle phenotype of cnn^HK21

(Wainman et al., 2009)

Other

Statement

Reference

cnn^HK21, msd1^ex51 has lethal phenotype

(Wainman et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

cnn^HK21;msd1^ex51 double mutants are homozygous lethal, dying at an early pupal stage. cnn^HK21;msd1^ex51 mutants show "metaphase-like" cells with weak, unorganized microtubule arrays; very few robust spindles are observed. An increased mitotic index is observed in these double mutants, greater than the increase seen in either cnn^HK21 or msd1^ex51 single mutants, and a dramatic increase in metaphase-to-anaphase ratio. Microtubule nucleation around chromatin in cnn^HK21;msd1^ex51 mutants is still observed, similar to that seen during prometaphase in cnn^HK21 mutants, suggesting that nucleation of microtubules from around mitotic chromatin is insufficient for viability.

(Wainman et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

msd1^ex51 is rescued by msd1^UAS.GFP/Scer\GAL4^{VP16.mat.αTub67C}

(Wainman et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

msd1^ex51

(Hayward et al., 2014, Wainman et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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