Allele Dmel\yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}

General Information
Symbol	Dmel\yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0239730
Feature type	allele	Associated gene	Dmel\yki

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Oh and Irvine, 2008) in vitro construct - regulatory fusion (Oh and Irvine, 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of yki which is tagged with T:Avic\GFP[EGFP] and is tagged at the C-terminal end with T:Ivir\HA1. (Oh and Irvine, 2008)
Carried in construct	P{UAS-yki.GFP} (Oh and Irvine, 2008, Fernández et al., 2011, Chen and Verheyen, 2012)
Tagged with	T:Avic\GFP^EGFP (Oh and Irvine, 2008) T:Ivir\HA1 (Oh and Irvine, 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of yki[Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1] under the control of Scer\GAL4[GMR.PF] does not result in any obvious eye overgrowth. Clones in the wing disc expressing yki[Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1] under the control of Scer\GAL4[Act5C.PI] show irregular shapes and are normal in size. (Oh and Irvine, 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^hh-Gal4, yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} has size defective phenotype, enhanceable by Scer\GAL4^hh-Gal4/hipk^{Scer\UAS.T:Ivir\HA1} (Chen and Verheyen, 2012)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^hh-Gal4, yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} has wing disc phenotype, enhanceable by Scer\GAL4^hh-Gal4/hipk^{Scer\UAS.T:Ivir\HA1} (Chen and Verheyen, 2012)
NOT Suppressor of
Statement Reference Scer\GAL4^Act5C.PI, yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}, Scer\GAL4^Act5C.PI is a non-suppressor of wing disc \| somatic clone phenotype of Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hpo^{Scer\UAS.T:Zzzz\FLAG} (Oh and Irvine, 2008)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of hipk[Scer\UAS.T:Ivir\HA1] and yki[Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1] under the control of Scer\GAL4[hh-Gal4] enhances the wing disc overgrowth seen when either construct is expressed alone. (Chen and Verheyen, 2012) Clones co-expressing hpo[Scer\UAS.T:Zzzz\FLAG] and yki[Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1] under the control of Scer\GAL4[Act5C.PI] in the wing disc are tiny and rarely detected. (Oh and Irvine, 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^ci.PC/yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} rescues yki^B5 (Oh and Irvine, 2008) yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}/Scer\GAL4^arm.PS rescues yki^B5 (Oh and Irvine, 2008)
Comments	Expression of yki[Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1] under the control of Scer\GAL4[arm.PS] rescues the lethality of yki[B5]. Expression of yki[Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1] under the control of Scer\GAL4[ci.PC] rescues the growth and viability of yki[B5] clones in the wing disc. (Oh and Irvine, 2008)
Stocks ( 1 )
Bloomington	28815 y¹ w^*; P{UAS-yki.GFP}4-12-1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Chen and Verheyen, 2012, Curr. Biol. 22(17): 1582--1586 Homeodomain-interacting protein kinase regulates yorkie activity to promote tissue growth. [FBrf0219448] Fernández et al., 2011, Development 138(11): 2337--2346 Actin-Capping Protein and the Hippo pathway regulate F-actin and tissue growth in Drosophila. [FBrf0213674] Oh and Irvine, 2008, Development 135(6): 1081--1088 In vivo regulation of Yorkie phosphorylation and localization. [FBrf0204358]

Allele Dmel\ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1

Allele Dmel\yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}