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Allele Dmel\yki^{S111A.S250A.Scer\UAS.T:SV5\V5}

General Information
Symbol	Dmel\ykiS111A.S250A.Scer\UAS.T:SV5\V5	Species	D. melanogaster
Name		FlyBase ID	FBal0239739
Feature type	allele	Associated gene	Dmel\yki
Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Oh and Irvine, 2009) in vitro construct - coding region fusion (Oh and Irvine, 2009) in vitro construct - regulatory fusion (Oh and Irvine, 2009)
Mutations Mapped to the Genome
Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a mutated form of yki which is tagged with T:SV5\V5. (Oh and Irvine, 2009) Amino acid replacement: S111A. (Oh and Irvine, 2009) Amino acid replacement: S250A. (Oh and Irvine, 2009)
Carried in construct	P{UAS-yki.S111A.S250A.V5} (Oh and Irvine, 2009, Oh and Irvine, 2011)
Tagged with	T:SV5\V5 (Oh and Irvine, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell number | somatic clone, with Scer\GAL4Scer\FRT.Act5C (Oh and Irvine, 2011) lethal | partially, with Scer\GAL4sd-SG29.1 (Oh and Irvine, 2009) size defective | adult stage, with Scer\GAL4GMR.PF (Oh and Irvine, 2011) visible, with Scer\GAL4GMR.PF (Oh and Irvine, 2009) visible, with Scer\GAL4sd-SG29.1 (Oh and Irvine, 2009) visible | adult stage, with Scer\GAL4GMR.PF (Oh and Irvine, 2011)
Phenotype Manifest In
	eye, with Scer\GAL4GMR.PF (Oh and Irvine, 2009, Oh and Irvine, 2011) wing, with Scer\GAL4sd-SG29.1 (Oh and Irvine, 2009) wing disc | somatic clone, with Scer\GAL4Act5C.PI (Oh and Irvine, 2009) wing disc | somatic clone, with Scer\GAL4Scer\FRT.Act5C (Oh and Irvine, 2011)
Detailed Description
	Statement Reference Expression of yki[S111A.S250A.Scer\UAS.T:SV5\V5] under the control of Scer\GAL4[GMR.PF] produces enlarged and irregular eyes. Wing disc clones expressing yki[S111A.S250A.Scer\UAS.T:SV5\V5] under the control of Scer\GAL4[Scer\FRT.Act5C] induce mild growth enhancement. (Oh and Irvine, 2011) Expression of yki[S111A.S250A.Scer\UAS.T:SV5\V5] under the control of Scer\GAL4[GMR.PF] results in an increase in the size of the eye and the eye is mostly flat. Expression of yki[S111A.S250A.Scer\UAS.T:SV5\V5] under the control of Scer\GAL4[sd-SG29.1] results in low viability and distorted wings. Clones in the wing disc expressing yki[S111A.S250A.Scer\UAS.T:SV5\V5] under the control of Scer\GAL4[Act5C.PI] are rounder than normal. (Oh and Irvine, 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has size defective | adult stage phenotype, enhanceable by MadScer\UAS.T:Zzzz\FLAG, Scer\GAL4GMR.PF (Oh and Irvine, 2011) Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has size defective | adult stage phenotype, enhanceable by tkvQ253D.Scer\UAS.cNc/Scer\GAL4GMR.PF (Oh and Irvine, 2011) Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has visible | adult stage phenotype, enhanceable by MadScer\UAS.T:Zzzz\FLAG, Scer\GAL4GMR.PF (Oh and Irvine, 2011) Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has visible | adult stage phenotype, enhanceable by tkvQ253D.Scer\UAS.cNc/Scer\GAL4GMR.PF (Oh and Irvine, 2011) Scer\GAL4Scer\FRT.Act5C, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has increased cell number | somatic clone phenotype, enhanceable by tkvQ253D.Scer\UAS.cNc/MadScer\UAS.T:Zzzz\FLAG, Scer\GAL4Scer\FRT.Act5C (Oh and Irvine, 2011)
Enhancer of
Statement Reference ykiS111A.S250A.Scer\UAS.T:SV5\V5, Scer\GAL4Scer\FRT.Act5C is an enhancer of increased cell number | somatic clone phenotype of MadScer\UAS.T:Zzzz\FLAG, Scer\GAL4Scer\FRT.Act5C, tkvQ253D.Scer\UAS.cNc (Oh and Irvine, 2011)
Other
Statement Reference Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hpoScer\UAS.T:Zzzz\FLAG, wtsScer\UAS.T:Hsap\MYC, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has visible phenotype (Oh and Irvine, 2009) Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hpoScer\UAS.T:Zzzz\FLAG, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has visible phenotype (Oh and Irvine, 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has eye phenotype, enhanceable by MadScer\UAS.T:Zzzz\FLAG, Scer\GAL4GMR.PF (Oh and Irvine, 2011) Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has eye phenotype, enhanceable by tkvQ253D.Scer\UAS.cNc/Scer\GAL4GMR.PF (Oh and Irvine, 2011) Scer\GAL4Scer\FRT.Act5C, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has wing disc | somatic clone phenotype, enhanceable by tkvQ253D.Scer\UAS.cNc/MadScer\UAS.T:Zzzz\FLAG, Scer\GAL4Scer\FRT.Act5C (Oh and Irvine, 2011)
Enhancer of
Statement Reference ykiS111A.S250A.Scer\UAS.T:SV5\V5, Scer\GAL4Scer\FRT.Act5C is an enhancer of wing disc | somatic clone phenotype of MadScer\UAS.T:Zzzz\FLAG, Scer\GAL4Scer\FRT.Act5C, tkvQ253D.Scer\UAS.cNc (Oh and Irvine, 2011)
Other
Statement Reference Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI, hpoScer\UAS.T:Zzzz\FLAG, wtsScer\UAS.T:Hsap\MYC, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has wing disc | somatic clone phenotype (Oh and Irvine, 2009) Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hpoScer\UAS.T:Zzzz\FLAG, wtsScer\UAS.T:Hsap\MYC, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has eye phenotype (Oh and Irvine, 2009) Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hpoScer\UAS.T:Zzzz\FLAG, ykiS111A.S250A.Scer\UAS.T:SV5\V5 has eye phenotype (Oh and Irvine, 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of Mad[Scer\UAS.T:Zzzz\FLAG] enhances the eye overgrowth seen when yki[S111A.S250A.Scer\UAS.T:SV5\V5] is expressed under the control of Scer\GAL4[GMR.PF]. Expression of tkv[Q253D.Scer\UAS.cNc] enhances the eye overgrowth seen when yki[S111A.S250A.Scer\UAS.T:SV5\V5] is expressed under the control of Scer\GAL4[GMR.PF]. Expression of yki[S111A.S250A.Scer\UAS.T:SV5\V5] enhances the overgrowth seen in wing disc clones expressing Mad[Scer\UAS.T:Zzzz\FLAG] and tkv[Q253D.Scer\UAS.cNc] under the control of Scer\GAL4[Scer\FRT.Act5C]. The phenotype seen in stronger than is seen when yki[S111A.S250A.Scer\UAS.T:SV5\V5] is expressed alone. (Oh and Irvine, 2011) Co-expression of yki[S111A.S250A.Scer\UAS.T:SV5\V5] and hpo[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] results in flies with small eyes. Co-expression of yki[S111A.S250A.Scer\UAS.T:SV5\V5], wts[Scer\UAS.T:Hsap\MYC] and hpo[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] results in flies with very small eyes. Clones in the wing disc co-expressing yki[S111A.S250A.Scer\UAS.T:SV5\V5], wts[Scer\UAS.T:Hsap\MYC] and hpo[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[Act5C.PI] do not survive well. (Oh and Irvine, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )
Notes on Origin
Discoverer
External Crossreferences & Linkouts
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ykiS111A.S250A.Scer\UAS.T:SV5\V5
Name Synonym
Secondary FlyBase IDs
References ( 2 )
Research paper	Oh and Irvine, 2011, Dev. Cell 20(1): 109--122 Cooperative Regulation of Growth by Yorkie and Mad through bantam. [FBrf0212763] Oh and Irvine, 2009, Oncogene 28(17): 1916--1927 In vivo analysis of Yorkie phosphorylation sites. [FBrf0207827]