FB2019_05, released Oct 15, 2019

Allele: Dmel\ykiS168A.S250A.UAS.Tag:V5

Open Close
General Information
Symbol
Dmel\ykiS168A.S250A.UAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0239742
Feature type
allele
Associated gene
Dmel\yki
Associated Insertion(s)
Carried in Construct
P{UAS-yki.S168A.S250A.V5}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Oh and Irvine, 2009)
in vitro construct - coding region fusion
(Oh and Irvine, 2009)
in vitro construct - amino acid replacement
(Oh and Irvine, 2009)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-yki.S168A.S250A.V5}
      Cytology
      Nature of the lesion
      Statement
      Reference
      Amino acid replacement: S168A.
      (Oh and Irvine, 2009)
      UAS regulatory sequences drive expression of a mutated form of yki which is tagged with Tag:V5.
      (Oh and Irvine, 2009)
      Amino acid replacement: S250A.
      (Oh and Irvine, 2009)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      yki
      Tagged with
      Tag:V5
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      Expression of ykiS168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF results in a greatly enlarged and folded eye, and viability is reduced. Expression of ykiS168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4sd-SG29.1 results in an extended third instar larval stage and eventual late larval lethality.
      (Oh and Irvine, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Co-expression of ykiS168A.S250A.Scer\UAS.T:SV5\V5 and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of hpoScer\UAS.T:Zzzz\FLAG). Co-expression of ykiS168A.S250A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG).
      (Oh and Irvine, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ykiS168A.S250A.Scer\UAS.T:SV5\V5
      ykiS168A.S250A.UAS.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (1)