FlyBase Allele Report: Dmel\yki[S111A.S168A.S250A.UAS.Tag:V5]

General Information

Symbol

Dmel\yki^{S111A.S168A.S250A.UAS.Tag:V5}

Species

D. melanogaster

Name

FlyBase ID

FBal0239743

Feature type

allele

Associated gene

Dmel\yki

Associated Insertion(s)

Carried in Construct

P{UAS-yki.S111A.S168A.S250A.V5}

Also Known As

UAS-Yki^{S111A,S168A,S250A}, UAS-yki.S111A.S168A.S250A.V5, UAS-yki^{S111A.S168A.S250A.V5}, UAS-Yki^{S111A.S168A.S250A}, UAS-Yki^3SA

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Oh and Irvine, 2009)

in vitro construct - coding region fusion

(Oh and Irvine, 2009)

in vitro construct - amino acid replacement

(Oh and Irvine, 2009)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-yki.S111A.S168A.S250A.V5}

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: S111A.

(Oh and Irvine, 2009)

UAS regulatory sequences drive expression of a mutated form of yki which is tagged with Tag:V5.

(Oh and Irvine, 2009)

Amino acid replacement: S168A.

(Oh and Irvine, 2009)

Amino acid replacement: S250A.

(Oh and Irvine, 2009)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

yki

Tagged with

Tag:V5

epitope tag

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Napoletano et al., 2011)

model of intestinal cancer

CEA

(Bajpai et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intestinal cancer

is ameliorated by mew^JF02694

(Bajpai et al., 2020)

is ameliorated by rhea^HM05161

(Bajpai et al., 2020)

is ameliorated by vg^TONDU.UAS

(Bajpai et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} in adult intestinal stem cells results in increased cell proliferation, leading to systemic organ wasting that affects the ovary, fat body and muscle.

(Kwon et al., 2015)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^NP5130

(Bajpai et al., 2020, Kwon et al., 2015)

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^GMR.PF

(Napoletano et al., 2011)

abnormal size | larval stage, with Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

atrophy | oogenesis, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

increased cell number, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Kwon et al., 2013)

increased cell number | adult stage, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

increased cell size, with Scer\GAL4^tin.CΔ4

(Yu et al., 2015)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

lethal, with Scer\GAL4^GMR.PF

(Oh and Irvine, 2009)

lethal, with Scer\GAL4^vg.PM

(Oh and Irvine, 2009)

lethal - all die before end of larval stage, with Scer\GAL4^sd-SG29.1

(Oh and Irvine, 2009)

neoplasia | adult stage, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

neoplasia | larval stage, with Scer\GAL4^GMR.PU

(Oh et al., 2009)

neoplasia | larval stage | somatic clone, with Scer\GAL4^Act5C.PI

(Oh et al., 2009)

partially lethal - majority die, with Scer\GAL4^GMR.PF

(Tyra et al., 2020)

short lived, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

some die during larval stage, with Scer\GAL4^sd-SG29.1

(Oh and Irvine, 2009)

visible, with Scer\GAL4^GMR.PF

(Oh and Irvine, 2009)

visible, with Scer\GAL4^GMR.PF, Scer\GAL80^αTub84B.PL

(Oh and Irvine, 2009)

visible, with Scer\GAL4^NP5130

(Kwon et al., 2015)

visible | adult stage, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

Phenotype Manifest In

adult abdomen, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

adult heart, with Scer\GAL4^tin.CΔ4

(Yu et al., 2015)

adult hemolymph, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

adult midgut, with Scer\GAL4^NP5130

(Kwon et al., 2015)

adult midgut epithelium, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

eye, with Scer\GAL4^ey.PH

(Wan et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Oh and Irvine, 2009)

eye, with Scer\GAL4^GMR.PF, Scer\GAL80^αTub84B.PL

(Oh and Irvine, 2009)

eye disc | larval stage, with Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

eye disc | larval stage, with Scer\GAL4^GMR.PU

(Oh et al., 2009)

eye photoreceptor cell | progressive, with Scer\GAL4^GMR.PF

(Napoletano et al., 2011)

fat body, with Scer\GAL4^NP5130

(Kwon et al., 2015)

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Kwon et al., 2013)

mitochondrion, with Scer\GAL4^NP5130

(Kwon et al., 2015)

ovary, with Scer\GAL4^NP5130

(Kwon et al., 2015)

ovary | adult stage, with Scer\GAL4^NP5130

(Bajpai et al., 2020)

wing, with Scer\GAL4^NP5130

(Kwon et al., 2015)

wing pouch | larval stage | somatic clone, with Scer\GAL4^Act5C.PI

(Oh et al., 2009)

Detailed Description

Statement

Reference

Adulthood-only expression of yki^{S111A.S168A.S250A.UAS.Tag:V5} under the control of Scer\GAL4^NP5130 leads to gut stem cell-derived tumors, associated with abdominal bloating and wasting phenotypes: ovarian atrophy, increased hemolymph volume and decreased climbing activity.

(Bajpai et al., 2020)

Third instar larvae expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^RapGAP1-OK6 show normal number of satellite boutons on neuromuscular junctions relative to controls.

(Sakuma et al., 2016)

Expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} in adult midgut intestinal stem cells (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) results in massive cell proliferation resulting in a bloating phenotype. This progressive phenotype is seen from 5 days after yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} induction onwards. The ovaries of these flies degenerate progressively, and in contrast to the continuous mass seen in wild type flies the fat bodies of flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} appear as discontinuous patches that later degenerate completely. Progressive climbing defects and downturned wings are also seen. The mitochondria of muscles expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} are swollen and often associated with empty space. Mitochondrial degeneration is seen.

The volume of extractable hemolymph from flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} in adult midgut intestinal stem cells (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is greatly increased compared to controls. The concentration of trehalose in the hemolymph is significantly increased. Whole-body trehalose levels are increased. Whole-body triglycerides and glycogen are reduced compared to controls. Food intake and excretion are unaffected.

Increased glucose incorporation is seen in the midgut of flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} in adult midgut intestinal stem cells (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) compared with controls. In other parts of these flies, glucose incorporation is reduced.

(Kwon et al., 2015)

Scer\GAL4^ey.PH-mediated expression results in tumourous eyes.

(Wan et al., 2015)

The heart walls of flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^tin.CΔ4 are thicker than controls but cardiomyocyte number is the same as in controls.

(Yu et al., 2015)

When yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is expressed under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B, midgut stem cells over-proliferate.

(Kwon et al., 2013)

Expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GMR.PFa produces enlarged larval eye discs with folds of excess tissue.

(Gilbert et al., 2011)

Expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GMR.PF during the adult stage (using the a temperature-sensitive Scer\GAL80 line to control the timing of expression) results in unequivocal, albeit weak, progressive degeneration of photoreceptor cells.

(Napoletano et al., 2011)

Expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GMR.PF results in greatly reduced viability, with the eyes being enlarged and highly folded in surviving adults.

Expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^sd-SG29.1 results in early larval lethality.

Flies carrying yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} in the presence of Scer\GAL4^GMR.PF and Scer\GAL80^αTub84B.PL show obvious eye overgrowth.

(Oh and Irvine, 2009)

Clones of cells in the wing pouch in which yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is over-expressed overgrow compared to control clones.

Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} results in overgrowth of the eye disc, which is mostly taken up in extensive folds of tissue.

(Oh et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | adult stage phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has short lived phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased occurrence of cell division | adult stage phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has atrophy | oogenesis phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | adult stage phenotype, suppressible | partially by mew^JF02694/Scer\GAL4^NP5130

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased cell number | adult stage phenotype, suppressible | partially by mew^JF02694/Scer\GAL4^NP5130

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | adult stage phenotype, suppressible | partially by rhea^HM05161/Scer\GAL4^NP5130

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased cell number | adult stage phenotype, suppressible | partially by rhea^HM05161/Scer\GAL4^NP5130

(Bajpai et al., 2020)

Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased mortality during development phenotype, suppressible by Acn^UAS.cNa, Scer\GAL4^GMR.PF

(Tyra et al., 2020)

Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased mortality during development phenotype, suppressible by Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Tyra et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has visible phenotype, suppressible by ImpL2^NIG.15009R, Scer\GAL4^NP5130

(Kwon et al., 2015)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has abnormal locomotor behavior | adult stage phenotype, suppressible by ImpL2^NIG.15009R, Scer\GAL4^NP5130

(Kwon et al., 2015)

Scer\GAL4^ey.PH, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia phenotype, suppressible by BuGZ^GD9485, Scer\GAL4^ey.PH

(Wan et al., 2015)

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased cell number phenotype, suppressible by Leash^UAS.cKa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Kwon et al., 2013)

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased cell number phenotype, suppressible by Hsap\ARRDC3^UAS.cKa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Kwon et al., 2013)

Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has abnormal size | larval stage phenotype, suppressible by mop^UAS.cMa/Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5} has lethal phenotype, suppressible by hpo^UAS.Tag:FLAG/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5} has lethal phenotype, suppressible by hpo^UAS.Tag:FLAG/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF/wts^UAS.Tag:MYC

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | larval stage phenotype, suppressible | partially by hpo^UAS.Tag:FLAG/wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | larval stage phenotype, suppressible | partially by hpo^UAS.Tag:FLAG/ex^UAS.cBa/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^Act5C.PI, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | somatic clone | larval stage phenotype, suppressible by hpo^UAS.Tag:FLAG/ex^UAS.cBa/Scer\GAL4^Act5C.PI/wts^UAS.Tag:MYC

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | larval stage phenotype, suppressible by hpo^UAS.Tag:FLAG/ex^UAS.cBa/wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | larval stage phenotype, non-suppressible by hpo^UAS.Tag:FLAG/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | larval stage phenotype, non-suppressible by ex^UAS.cBa/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | larval stage phenotype, non-suppressible by ex^UAS.cBa/wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has neoplasia | larval stage phenotype, non-suppressible by wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Other

Statement

Reference

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S168A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S168A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has adult midgut epithelium phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has adult abdomen phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has adult hemolymph phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has ovary | adult stage phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has intestinal stem cell | adult stage phenotype, suppressible | partially by Scer\GAL4^NP5130/vg^TONDU.UAS

(Bajpai et al., 2020)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has ovary phenotype, suppressible by ImpL2^NIG.15009R, Scer\GAL4^NP5130

(Kwon et al., 2015)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has wing phenotype, suppressible by ImpL2^NIG.15009R, Scer\GAL4^NP5130

(Kwon et al., 2015)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has mitochondrion phenotype, suppressible by ImpL2^NIG.15009R, Scer\GAL4^NP5130

(Kwon et al., 2015)

Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5} has ovary phenotype, suppressible by ImpL2^Def20

(Kwon et al., 2015)

Scer\GAL4^ey.PH, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye phenotype, suppressible by BuGZ^GD9485, Scer\GAL4^ey.PH

(Wan et al., 2015)

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, yki^{S111A.S168A.S250A.UAS.Tag:V5} has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Leash^UAS.cKa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Kwon et al., 2013)

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, yki^{S111A.S168A.S250A.UAS.Tag:V5} has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Hsap\ARRDC3^UAS.cKa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Kwon et al., 2013)

Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, suppressible by mop^UAS.cMa/Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, suppressible | partially by hpo^UAS.Tag:FLAG/wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, suppressible | partially by hpo^UAS.Tag:FLAG/ex^UAS.cBa/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^Act5C.PI, yki^{S111A.S168A.S250A.UAS.Tag:V5} has wing pouch | somatic clone | larval stage phenotype, suppressible by hpo^UAS.Tag:FLAG/ex^UAS.cBa/Scer\GAL4^Act5C.PI/wts^UAS.Tag:MYC

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, suppressible by hpo^UAS.Tag:FLAG/ex^UAS.cBa/wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, non-suppressible by hpo^UAS.Tag:FLAG/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, non-suppressible by ex^UAS.cBa/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, non-suppressible by ex^UAS.cBa/wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, non-suppressible by wts^UAS.Tag:MYC/Scer\GAL4^GMR.PU

(Oh et al., 2009)

Other

Statement

Reference

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

ImpL2^Def20 suppresses the increase in circulating trehalose concentration and whole-body trehalose levels seen in adult intestinal stem cells expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B).

Expression of ImpL2^NIG.15009R suppresses the increase in trehalose levels seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B). The overall shape of the midguts is unchanged.

The wasting of ovaries seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is suppressed by ImpL2^NIG.15009R.

The climbing defects and downturned wings seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) are suppressed by ImpL2^NIG.15009R.

The mitochondrial defects seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) are suppressed by ImpL2^NIG.15009R. Muscle ATP levels are also reduced.

The bloating phenotype seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is suppressed by ImpL2^NIG.15009R.

The reduction in glucose levels seen in the body void (excluding the midgut and hemolymph) of flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is suppressed by ImpL2^Def20.

Expression of ImpL2^GD6004 partially suppresses the increase in circulating and whole-body trehalose levels seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B). The overall shape of the midguts is unchanged.

(Kwon et al., 2015)

Co-expression of mop^Scer\UAS.cMa suppresses the larval eye disc overgrowth seen when yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is expressed under the control of Scer\GAL4^GMR.PFa.

(Gilbert et al., 2011)

Co-expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with enlarged and highly folded eyes.

Co-expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with enlarged and highly folded eyes.

Clones co-expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} ex^Scer\UAS.cBa, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI in the wing disc are large and round.

(Oh and Irvine, 2009)

Clones of cells in the wing or eye disc in which wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa are over-expressed together with yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} generally overgrow compared to control clones, but can be less overgrown than yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5}-expressing clones alone. This is most evident in the wing pouch.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is largely suppressed by co-expression of wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is not suppressed by co-expression of wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} or ex^Scer\UAS.cBa alone, nor by co-expression of wts^{Scer\UAS.T:Hsap\MYC} and ex^Scer\UAS.cBa together.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is partly suppressed by co-expression of wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} together, or hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa together.

(Oh et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

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Mutant

Wild-type

Stocks (1)

Bloomington

28817

w^*; P{UAS-yki.S111A.S168A.S250A.V5}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Yki^3SA

(Bajpai et al., 2020)

yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5}

yki^{S111A.S168A.S250A.UAS.Tag:V5}

Name Synonyms

Secondary FlyBase IDs

References (26)

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