FB2019_05, released Oct 15, 2019

Allele: Dmel\ykiS111A.S168A.S250A.UAS.Tag:V5

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General Information
Symbol
Dmel\ykiS111A.S168A.S250A.UAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0239743
Feature type
allele
Associated gene
Dmel\yki
Associated Insertion(s)
Carried in Construct
P{UAS-yki.S111A.S168A.S250A.V5}
Also Known As
UAS-yki.S111A.S168A.S250A.V5, UAS-ykiS111A.S168A.S250A.V5
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Oh and Irvine, 2009)
in vitro construct - coding region fusion
(Oh and Irvine, 2009)
in vitro construct - amino acid replacement
(Oh and Irvine, 2009)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-yki.S111A.S168A.S250A.V5}
      Cytology
      Nature of the lesion
      Statement
      Reference
      Amino acid replacement: S111A.
      (Oh and Irvine, 2009)
      UAS regulatory sequences drive expression of a mutated form of yki which is tagged with Tag:V5.
      (Oh and Irvine, 2009)
      Amino acid replacement: S168A.
      (Oh and Irvine, 2009)
      Amino acid replacement: S250A.
      (Oh and Irvine, 2009)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      yki
      Tagged with
      Tag:V5
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  neurodegenerative disease
      CEA
      (Napoletano et al., 2011)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
       
      Expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 in adult intestinal stem cells results in increased cell proliferation, leading to systemic organ wasting that affects the ovary, fat body and muscle.
      (Kwon et al., 2015)
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      Third instar larvae expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4RapGAP1-OK6 show normal number of satellite boutons on neuromuscular junctions relative to controls.
      (Sakuma et al., 2016)
      Expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 in adult midgut intestinal stem cells (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) results in massive cell proliferation resulting in a bloating phenotype. This progressive phenotype is seen from 5 days after ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 induction onwards. The ovaries of these flies degenerate progressively, and in contrast to the continuous mass seen in wild type flies the fat bodies of flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 appear as discontinuous patches that later degenerate completely. Progressive climbing defects and downturned wings are also seen. The mitochondria of muscles expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 are swollen and often associated with empty space. Mitochondrial degeneration is seen. The volume of extractable hemolymph from flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 in adult midgut intestinal stem cells (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) is greatly increased compared to controls. The concentration of trehalose in the hemolymph is significantly increased. Whole-body trehalose levels are increased. Whole-body triglycerides and glycogen are reduced compared to controls. Food intake and excretion are unaffected. Increased glucose incorporation is seen in the midgut of flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 in adult midgut intestinal stem cells (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) compared with controls. In other parts of these flies, glucose incorporation is reduced.
      (Kwon et al., 2015)
      Scer\GAL4ey.PH-mediated expression results in tumourous eyes.
      (Wan et al., 2015)
      The heart walls of flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4tin.CΔ4 are thicker than controls but cardiomyocyte number is the same as in controls.
      (Yu et al., 2015)
      When ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is expressed under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B, midgut stem cells over-proliferate.
      (Kwon et al., 2013)
      Expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PFa produces enlarged larval eye discs with folds of excess tissue.
      (Gilbert et al., 2011)
      Expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF during the adult stage (using the a temperature-sensitive Scer\GAL80 line to control the timing of expression) results in unequivocal, albeit weak, progressive degeneration of photoreceptor cells.
      (Napoletano et al., 2011)
      Expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF results in greatly reduced viability, with the eyes being enlarged and highly folded in surviving adults. Expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4sd-SG29.1 results in early larval lethality. Flies carrying ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 in the presence of Scer\GAL4GMR.PF and Scer\GAL80αTub84B.PL show obvious eye overgrowth.
      (Oh and Irvine, 2009)
      Clones of cells in the wing pouch in which ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is over-expressed overgrow compared to control clones. Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 results in overgrowth of the eye disc, which is mostly taken up in extensive folds of tissue.
      (Oh et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference
      Scer\GAL4NP5130, ykiS111A.S168A.S250A.UAS.Tag:V5 has visible phenotype, suppressible by ImpL2NIG.15009R, Scer\GAL4NP5130
      (Kwon et al., 2015)
      Scer\GAL4NP5130, ykiS111A.S168A.S250A.UAS.Tag:V5 has locomotor behavior defective | adult stage phenotype, suppressible by ImpL2NIG.15009R, Scer\GAL4NP5130
      (Kwon et al., 2015)
      Scer\GAL4ey.PH, ykiS111A.S168A.S250A.UAS.Tag:V5 has tumorigenic phenotype, suppressible by BuGZGD9485, Scer\GAL4ey.PH
      (Wan et al., 2015)
      Scer\GAL4NP5130, Scer\GAL80ts.αTub84B, ykiS111A.S168A.S250A.UAS.Tag:V5 has increased cell number phenotype, suppressible by LeashUAS.cKa, Scer\GAL4NP5130, Scer\GAL80ts.αTub84B
      (Kwon et al., 2013)
      Scer\GAL4NP5130, Scer\GAL80ts.αTub84B, ykiS111A.S168A.S250A.UAS.Tag:V5 has increased cell number phenotype, suppressible by Hsap\ARRDC3UAS.cKa, Scer\GAL4NP5130, Scer\GAL80ts.αTub84B
      (Kwon et al., 2013)
      Scer\GAL4GMR.PFa, ykiS111A.S168A.S250A.UAS.Tag:V5 has size defective | larval stage phenotype, suppressible by mopUAS.cMa/Scer\GAL4GMR.PFa
      (Gilbert et al., 2011)
      Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S168A.S250A.UAS.Tag:V5 has lethal phenotype, suppressible by hpoUAS.Tag:FLAG/Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
      (Oh and Irvine, 2009)
      Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, ykiS111A.S168A.S250A.UAS.Tag:V5 has lethal phenotype, suppressible by hpoUAS.Tag:FLAG/Scer\GAL4GMR.PF/Scer\GAL4GMR.PF/wtsUAS.Tag:MYC
      (Oh and Irvine, 2009)
      Scer\GAL4GMR.PU, ykiS111A.S168A.S250A.UAS.Tag:V5 has neoplasia | larval stage phenotype, suppressible | partially by hpoUAS.Tag:FLAG/wtsUAS.Tag:MYC/Scer\GAL4GMR.PU
      (Oh et al., 2009)
      Scer\GAL4GMR.PU, ykiS111A.S168A.S250A.UAS.Tag:V5 has neoplasia | larval stage phenotype, suppressible | partially by hpoUAS.Tag:FLAG/exUAS.cBa/Scer\GAL4GMR.PU
      (Oh et al., 2009)
      Scer\GAL4Act5C.PI, ykiS111A.S168A.S250A.UAS.Tag:V5 has neoplasia | somatic clone | larval stage phenotype, suppressible by hpoUAS.Tag:FLAG/exUAS.cBa/Scer\GAL4Act5C.PI/wtsUAS.Tag:MYC
      (Oh et al., 2009)
      Scer\GAL4GMR.PU, ykiS111A.S168A.S250A.UAS.Tag:V5 has neoplasia | larval stage phenotype, suppressible by hpoUAS.Tag:FLAG/exUAS.cBa/wtsUAS.Tag:MYC/Scer\GAL4GMR.PU
      (Oh et al., 2009)
      NOT suppressed by
      Other
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference
      Scer\GAL4NP5130, ykiS111A.S168A.S250A.UAS.Tag:V5 has ovary phenotype, suppressible by ImpL2NIG.15009R, Scer\GAL4NP5130
      (Kwon et al., 2015)
      Scer\GAL4NP5130, ykiS111A.S168A.S250A.UAS.Tag:V5 has wing phenotype, suppressible by ImpL2NIG.15009R, Scer\GAL4NP5130
      (Kwon et al., 2015)
      Scer\GAL4NP5130, ykiS111A.S168A.S250A.UAS.Tag:V5 has mitochondrion phenotype, suppressible by ImpL2NIG.15009R, Scer\GAL4NP5130
      (Kwon et al., 2015)
      Scer\GAL4NP5130, ykiS111A.S168A.S250A.UAS.Tag:V5 has ovary phenotype, suppressible by ImpL2Def20
      (Kwon et al., 2015)
      Scer\GAL4ey.PH, ykiS111A.S168A.S250A.UAS.Tag:V5 has eye phenotype, suppressible by BuGZGD9485, Scer\GAL4ey.PH
      (Wan et al., 2015)
      Scer\GAL4NP5130, Scer\GAL80ts.αTub84B, ykiS111A.S168A.S250A.UAS.Tag:V5 has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by LeashUAS.cKa, Scer\GAL4NP5130, Scer\GAL80ts.αTub84B
      (Kwon et al., 2013)
      Scer\GAL4NP5130, Scer\GAL80ts.αTub84B, ykiS111A.S168A.S250A.UAS.Tag:V5 has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Hsap\ARRDC3UAS.cKa, Scer\GAL4NP5130, Scer\GAL80ts.αTub84B
      (Kwon et al., 2013)
      Scer\GAL4GMR.PFa, ykiS111A.S168A.S250A.UAS.Tag:V5 has eye disc | larval stage phenotype, suppressible by mopUAS.cMa/Scer\GAL4GMR.PFa
      (Gilbert et al., 2011)
      Scer\GAL4GMR.PU, ykiS111A.S168A.S250A.UAS.Tag:V5 has eye disc | larval stage phenotype, suppressible | partially by hpoUAS.Tag:FLAG/wtsUAS.Tag:MYC/Scer\GAL4GMR.PU
      (Oh et al., 2009)
      Scer\GAL4GMR.PU, ykiS111A.S168A.S250A.UAS.Tag:V5 has eye disc | larval stage phenotype, suppressible | partially by hpoUAS.Tag:FLAG/exUAS.cBa/Scer\GAL4GMR.PU
      (Oh et al., 2009)
      Scer\GAL4Act5C.PI, ykiS111A.S168A.S250A.UAS.Tag:V5 has wing pouch | somatic clone | larval stage phenotype, suppressible by hpoUAS.Tag:FLAG/exUAS.cBa/Scer\GAL4Act5C.PI/wtsUAS.Tag:MYC
      (Oh et al., 2009)
      Scer\GAL4GMR.PU, ykiS111A.S168A.S250A.UAS.Tag:V5 has eye disc | larval stage phenotype, suppressible by hpoUAS.Tag:FLAG/exUAS.cBa/wtsUAS.Tag:MYC/Scer\GAL4GMR.PU
      (Oh et al., 2009)
      NOT suppressed by
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      ImpL2Def20 suppresses the increase in circulating trehalose concentration and whole-body trehalose levels seen in adult intestinal stem cells expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B). Expression of ImpL2NIG.15009R suppresses the increase in trehalose levels seen in flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B). The overall shape of the midguts is unchanged. The wasting of ovaries seen in flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) is suppressed by ImpL2NIG.15009R. The wasting of ovaries seen in flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) is suppressed by ImpL2Def20. The climbing defects and downturned wings seen in flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) are suppressed by ImpL2NIG.15009R. The mitochondrial defects seen in flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) are suppressed by ImpL2NIG.15009R. Muscle ATP levels are also reduced. The bloating phenotype seen in flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) is suppressed by ImpL2NIG.15009R. The reduction in glucose levels seen in the body void (excluding the midgut and hemolymph) of flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B) is suppressed by ImpL2Def20. Expression of ImpL2GD6004 partially suppresses the increase in circulating and whole-body trehalose levels seen in flies expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 (under the control of Scer\GAL4esg-NP5130 and limited to the adult stage using Scer\GAL80ts.αTub84B). The overall shape of the midguts is unchanged.
      (Kwon et al., 2015)
      Co-expression of mopScer\UAS.cMa suppresses the larval eye disc overgrowth seen when ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is expressed under the control of Scer\GAL4GMR.PFa.
      (Gilbert et al., 2011)
      Co-expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with enlarged and highly folded eyes. Co-expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with enlarged and highly folded eyes. Clones co-expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 exScer\UAS.cBa, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI in the wing disc are large and round.
      (Oh and Irvine, 2009)
      Clones of cells in the wing or eye disc in which wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa are over-expressed together with ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 generally overgrow compared to control clones, but can be less overgrown than ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5-expressing clones alone. This is most evident in the wing pouch. The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is largely suppressed by co-expression of wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa. The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is not suppressed by co-expression of wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG or exScer\UAS.cBa alone, nor by co-expression of wtsScer\UAS.T:Hsap\MYC and exScer\UAS.cBa together. The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is partly suppressed by co-expression of wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG together, or hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa together.
      (Oh et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5
      ykiS111A.S168A.S250A.UAS.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (16)