Allele Dmel\NFAT^Δab

General Information
Symbol	Dmel\NFAT^Δab	Species	D. melanogaster
Name		FlyBase ID	FBal0239771
Feature type	allele	Associated gene	Dmel\NFAT

Map ( GBrowse )
Allele class
Mutagen	Delta2-3
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Keyser et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References deletion X:13,534,968..13,545,066 comment=Reported as a deletion from 1078bp upstream to 9020bp downstream of the 30 end of the EP1541 insertion sequence. Position of insertion on reference sequence inferred by FlyBase curator. (Keyser et al., 2007)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}NFAT^EP1541 (Keyser et al., 2007)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion, resulting in a deletion from 1078bp upstream to 9020bp downstream of the original insertion site. This deletion spans the first two exons of the b transcript as well as the two following exons that are common to both the a and b transcripts. No transcription can be detected from the NFAT b promoter. An aberrant transcript can be detected from the a promoter, which is spliced out of frame directly from the first to the fourth exon and which produces a truncated open reading frame of 64 amino acids. (Keyser et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Keyser et al., 2007) neuroanatomy defective (Freeman et al., 2011) neurophysiology defective (Freeman et al., 2011) osmotic stress response defective \| larval stage (Keyser et al., 2007) viable (Keyser et al., 2007)
Phenotype Manifest In
	embryonic/larval anal pad (Keyser et al., 2007) synapse (Freeman et al., 2011)
Detailed Description
	Statement Reference NFAT[Δab] mutants (either homozygous or heterozygous) exhibit expanded synapses compared to controls while average muscle surface area remains unaffected. A NFAT[Δab] heterozygous background increases transmitter release (mean EJC amplitude) while spontaneous mini EJCs are unaffected. The number of active zones are not affected in these mutants. (Freeman et al., 2011) Mutants have no obvious morphological defects. Mutant larvae show reduced survival at high salt concentrations compared to control larvae. Survival of the mutant larvae is significantly reduced at 0.2M NaCl and at 0.4M NaCl only 5% of the mutant first instar larvae survive to adulthood. Mutant larvae show a remarkable swelling of the anal pads in hypotonic conditions. Mutant larvae have melanised posterior spiracles after treatment at 60[o]C for 10 minutes. Occasionally the anal pads or the anterior spiracle openings are also coloured. (Keyser et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	32652 w¹¹¹⁸ NFAT^Δab
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	NFAT^Δab (Keyser et al., 2007) NFAT^ΔAB (Freeman et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Freeman et al., 2011, Mol. Cell. Neurosci. 46(2): 535--547 NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila. [FBrf0212894] Keyser et al., 2007, Insect Biochem. Mol. Biol. 37(4): 356--362 The Drosophila NFAT homolog is involved in salt stress tolerance. [FBrf0200725]

Allele Dmel\NFATΔab

Allele Dmel\NFAT^Δab