FB2025_01 , released February 20, 2025
Allele: Dmel\AmunUAS.mRFP1
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General Information
Symbol
Dmel\AmunUAS.mRFP1
Species
D. melanogaster
Name
FlyBase ID
FBal0239806
Feature type
allele
Associated gene
Dmel\Amun
Associated Insertion(s)
Carried in Construct
P{UAS-Amun.mRFP}
Key Links
Transgenic product class
cDNA
(Shalaby et al., 2009)
wild_type
(Shalaby et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Shalaby et al., 2009)
wild_type
(Shalaby et al., 2009)
Mutagen
in vitro construct
(Shalaby et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Amun.mRFP}
Cytology
Description

UAS regulatory sequences drive expression of full length Amun (a SalI-NotI fragment derived from cDNA clone GH02702), containing a C-terminal mRFP1 tag.

(Shalaby et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Amun
Tagged with
mRFP1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      macrochaeta & mesothoracic tergum, with Scer\GAL4ptc-559.1

      (Shalaby et al., 2009)

      macrochaeta & mesothoracic tergum, with Scer\GAL4sca-537.4

      (Shalaby et al., 2009)

      macrochaeta & mesothoracic tergum | ectopic, with Scer\GAL4sca-537.4

      (Shalaby et al., 2009)

      macrochaeta & mesothoracic tergum | supernumerary, with Scer\GAL4sca-537.4

      (Shalaby et al., 2009)

      microchaeta & mesothoracic tergum, with Scer\GAL4pnr-MD237

      (Shalaby et al., 2009)

      microchaeta & mesothoracic tergum, with Scer\GAL4sr-md710

      (Shalaby et al., 2009)

      microchaeta & mesothoracic tergum | somatic clone, with Scer\GAL4Act5C.PI

      (Shalaby et al., 2009)

      neuron & mesothoracic tergum primordium | pupal stage, with Scer\GAL4pnr-MD237

      (Shalaby et al., 2009)

      sensory mother cell & mesothoracic tergum primordium | pupal stage, with Scer\GAL4pnr-MD237

      (Shalaby et al., 2009)

      trichogen cell & mesothoracic tergum primordium | pupal stage, with Scer\GAL4pnr-MD237

      (Shalaby et al., 2009)
      Detailed Description
      Statement
      Reference

      Expression of AmunScer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4sca-537.4 results in a combination of missing, extra, and misplaced macrochaetae.

      Expression of AmunScer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4ptc-559.1 results in missing macrochaetae.

      Expression of AmunScer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4sr-md710 or Scer\GAL4pnr-MD237 results in complete loss of microchaetae.

      After expression of AmunScer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4pnr-MD237, few/no neurons or shaft cells are discernible in the pnr expression domain of 31 hr APF pupal nota, and SOPs are absent in 15 hr APF pupal nota.

      Adults developing from larvae with AmunScer\UAS.T:Disc\RFP-mRFP-expressing clones induced with Scer\GAL4Act5C.PI exhibit numerous small patches of notal microchaeta loss.

      (Shalaby et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      AmunUAS.mRFP1, Scer\GAL4pnr-MD237 has microchaeta & mesothoracic tergum phenotype, suppressible | partially by acUAS.cPa, Scer\GAL4pnr-MD237

      (Shalaby et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of acScer\UAS.cPa with AmunScer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4pnr-MD237 significantly supresses the microchatae loss phenotype seen in AmunScer\UAS.T:Disc\RFP-mRFP, Scer\GAL4pnr-MD237 flies.

      (Shalaby et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      AmunUAS.mRFP1 is rescued by Scer\GAL4pnr-MD237/AmunGD9779

      (Shalaby et al., 2009)
      Rescues

      AmunUAS.mRFP1/Scer\GAL4pnr-MD237 rescues AmunGD9779

      (Shalaby et al., 2009)
      Comments

      Co-expression of AmunScer\UAS.T:Disc\RFP-mRFP with AmunGD9779 under the control of Scer\GAL4pnr-MD237 rescues the small microchaetae phenotype seen in AmunGD9779, Scer\GAL4pnr-MD237 flies, and also rescues the microchatae loss phenotype seen in AmunScer\UAS.T:Disc\RFP-mRFP, Scer\GAL4pnr-MD237 flies.

      Co-expression of acScer\UAS.cPa with AmunScer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4pnr-MD237 significantly rescues the microchatae loss phenotype seen in AmunScer\UAS.T:Disc\RFP-mRFP, Scer\GAL4pnr-MD237 flies.

      (Shalaby et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AmunScer\UAS.T:Disc\RFP-mRFP
      AmunUAS.mRFP1
      Name Synonyms
      Secondary FlyBase IDs
        References (1)